Abstract: A biological source of proteolytic enzymes such as a culture liquid is brought into contact with a biospecific sorbent prepared by covalent addition of a naturally-occurring polypeptide, viz. bacitracin, to agarose. The sorbed enzyme is eluted with salt buffer solutions.The method of the present invention makes it possible to recover a colorless enzyme; owing to the sorbent selectivity a substantial effect is achieved in a single-stage purification process. The method may be used for recovering proteolytic enzymes of various classes including carboxyl, serine, thiol, metal proteinases and exopeptidases. The novel biospecific sorbent enables purification of various proteolytic enzymes with a yield of from 75 to 95% and with an increased, by 2 to 500 times, activity depending on the purity of the starting material.