Abstract: A method for isolating cytisine from plant material includes dissolving the plant material in an alcohol to form a liquid mixture and acidifying the liquid mixture by addition of an acid. The method also includes concentrating the liquid mixture to form a concentrated aqueous solution and extracting the concentrated aqueous solution with a first extractant to form a purified aqueous concentrate. The method includes alkalizing the purified aqueous concentrate with an alkalizing agent to form an alkaline aqueous concentrate. The method further includes extracting the alkaline aqueous concentrate with a second extractant and removing the second extractant to obtain cytisine.

Abstract: A method for isolating cytisine from plant material includes dissolving the plant material in an alcohol to form a liquid mixture, acidifying the liquid mixture by addition of a mineral acid, and concentrating the liquid mixture to form a concentrated aqueous solution. The method also includes extracting the concentrated aqueous solution with a first extractant to form a purified aqueous concentrate, alkalizing the purified aqueous concentrate with an alkaloid to form an alkaline aqueous concentrate, and extracting the alkaline aqueous concentrate with a second extractant. The method further includes removing the second extractant to obtain cytisine.

Abstract: The invention concerns a method for isolation of cytisine from plant material. The method according to the invention, consists in extraction of the plant raw material by lower alcohol, acidified with mineral acid to pH within a range 1.5-3.5. The combined alcohol-aqueous extracts are concentrated under vacuum distillation till the alcohol is eliminated and after filtration they are extracted with chloroform, methylene chloride, butyl acetate or normal butanol in a ratio of 1:1. The purified from ballast substances acid aqueous concentrate is alkalized to pH 9-12 with alkaline hydroxide or ammonium hydroxide and the resulting alkaline aqueous concentrate is extracted at least twice with chloroform, methylene chloride, butyl acetate or normal butanol in a ratio of concentrate:extractant of 1:5 to 1:10. The combined organic extracts are evaporated to dryness, then acetone or ethyl acetate are added to obtain a suspension which is allowed to crystallize completely at 5-10° C., then it is filtered and dried.

Abstract: The invention concerns a method for isolation of cytisine from plant material. The method according to the invention, consists in extraction of the plant raw material by lower alcohol, acidified with mineral acid to pH within a range 1.5-3.5. The combined alcohol-aqueous extracts are concentrated under vacuum distillation till the alcohol is eliminated and after filtration they are extracted with chloroform, methylene chloride, butyl acetate or normal butanol in a ratio of 1:1. The purified from ballast substances acid aqueous concentrate is alkalized to pH 9-12 with alkaline hydroxide or ammonium hydroxide and the resulting alkaline aqueous concentrate is extracted at least twice with chloroform, methylene chloride, butyl acetate or normal butanol in a ratio of concentrate:extractant of 1:5 to 1:10. The combined organic extracts are evaporated to dryness, then acetone or ethyl acetate are added to obtain a suspension which is allowed to crystallize completely at 5-10° C., then it is filtered and dried.

Abstract: The invention discloses a new method for purifying cytisine from raw plant extracts that yields high purity. The method comprises the steps of dissolving raw plant material with a lower alcohol, such as methanol or ethanol, then acidifying with mineral acid to a pH between 1.5 and 3.5. The combined alcohol-aqueous extracts are concentrated under vacuum distillation until the alcohol is eliminated and after filtration they are extracted with chloroform, methylene chloride, butyl acetate or normal butanol in a ratio of 1:1. The purified from ballast substances acid aqueous concentrate is alkalized to a pH of between 9 and 12 with alkaline hydroxide or ammonium hydroxide and the resulting alkaline aqueous concentrate is extracted at least twice with chloroform, methylene chloride, butyl acetate or normal butanol in a ratio of concentrate to extractant of from 1:5 to 1:10.