Abstract: A polypyrrole-graphene/polyurethane antifouling coating as well as a preparation method and an application thereof are provided. The polypyrrole-graphene/polyurethane antifouling coating includes component A and component B, wherein the component A mainly includes the following raw materials in parts by weight: 500 parts of group-hydrolyzable polyurethane prepolymer, 25-50 parts of bentonite, 25-50 parts of titanium dioxide, 60-120 parts of zinc oxide, 15-30 parts of talcum powder, and 15-30 parts of polypyrrole-graphene nanofiller; and the component B mainly includes the following raw materials in parts by weight: 5-7 parts of leveling agent, 5-7 parts of defoamer, 6-15 parts of chain extender, 10-25 parts of silane coupling agent, and 1-2 parts of catalyst. The two-component polyurethane coating of the present invention has relatively good adhesion, collision resistance and cracking resistance, and the antifouling property and the antifouling durability can be improved.