Patents by Inventor Gaozhong Shen

Gaozhong Shen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Nucleic Acids, Cells, and Methods for Producing Secreted Proteins
    Publication number: 20150093495
    Abstract: A method for producing a secreted recombinant polypeptide sequence is provided. In some embodiments it comprises providing a recombinant microorganism comprising a recombinant nucleic acid comprising a first nucleic acid sequence encoding the recombinant polypeptide sequence operatively linked to a second nucleic acid sequence encoding a signal peptide; and culturing the recombinant microorganism in a culture medium under conditions sufficient for production and secretion of the recombinant protein by the recombinant microorganism. In some embodiments the coding sequence for the signal peptide is not native to the recombinant microorganism. In some embodiments the recombinant microorganism is photo synthetic. Also provided are recombinant photosynthetic microorganisms, isolated polypeptides comprising a signal peptide comprising an amino acid sequence disclosed herein, and isolated nucleic acids comprising a coding sequence for one of the signal peptides, among other things.
    Type: Application
    Filed: April 29, 2013
    Publication date: April 2, 2015
    Applicant: Pronutria, Inc.
    Inventors: Gaozhong Shen, David M. Young, Subhayu Basu, Katherine G. Gora, Carine Robichon-Iyer, Nathaniel W. Silver, David Arthur Berry
  • Microorganisms, Compositions, and Methods for Producing Fatty Acids
    Publication number: 20150057352
    Abstract: Methods of producing an unsaturated free fatty acid comprising at least 18 carbon atoms are provided. In some embodiments, the methods comprise culturing an engineered microorganism in a culture medium, wherein the engineered microorganism comprises at least one recombinant enzyme selected from acyl-lipid desaturase delta-9 (EC:1.14.19.1), acyl-lipid desaturase delta-12 (EC:1.14.19.6), acyl-lipid desaturase delta-15 (EC:1.14.19.-), and thioesterase (EC:3.1.2.14). Engineered microorganisms comprising at least one of those recombinant enzymes are also provided. The methods and organisms can be used to produce at least one free fatty acid selected from oleic acid, linoleic acid and ?-linolenic acid.
    Type: Application
    Filed: July 18, 2014
    Publication date: February 26, 2015
    Inventors: Subhayu Basu, Gaozhong Shen, Brett Adam Boghigian, David M. Young, David Arthur Berry, Geoffrey von Maltzahn
  • Microorganisms and methods for producing unsaturated fatty acids
    Patent number: 8790901
    Abstract: Methods of producing an unsaturated free fatty acid comprising at least 18 carbon atoms are provided. In some embodiments, the methods comprise culturing an engineered microorganism in a culture medium, wherein the engineered microorganism comprises at least one recombinant enzyme selected from acyl-lipid desaturase delta-9 (EC:1.14.19.1), acyl-lipid desaturase delta-12 (EC:1.14.19.6), acyl-lipid desaturase delta-15 (EC:1.14.19.-), and thioesterase (EC:3.1.2.14). Engineered microorganisms comprising at least one of those recombinant enzymes are also provided. The methods and organisms can be used to produce at least one free fatty acid selected from oleic acid, linoleic acid and ?-linolenic acid.
    Type: Grant
    Filed: December 14, 2011
    Date of Patent: July 29, 2014
    Assignee: Pronutria, Inc.
    Inventors: Subhayu Basu, Gaozhong Shen, Brett Boghigian, David Young, David Berry, Geoffrey von Maltzahn
  • MICROORGANISMS AND METHODS FOR PRODUCING UNSATURATED FATTY ACIDS
    Publication number: 20120116108
    Abstract: Methods of producing an unsaturated free fatty acid comprising at least 18 carbon atoms are provided. In some embodiments, the methods comprise culturing an engineered microorganism in a culture medium, wherein the engineered microorganism comprises at least one recombinant enzyme selected from acyl-lipid desaturase delta-9 (EC:1.14.19.1), acyl-lipid desaturase delta-12 (EC:1.14.19.6), acyl-lipid desaturase delta-15 (EC:1.14.19.-), and thioesterase (EC:3.1.2.14). Engineered microorganisms comprising at least one of those recombinant enzymes are also provided. The methods and organisms can be used to produce at least one free fatty acid selected from oleic acid, linoleic acid and ?-linolenic acid.
    Type: Application
    Filed: December 14, 2011
    Publication date: May 10, 2012
    Applicant: ESSENTIENT, INC.
    Inventors: Subhayu Basu, Gaozhong Shen, Brett Boghigian