Abstract: A microfluidic device for use in a serial crystallography apparatus includes a modular 3D-printed nozzle having an inlet, an outlet, and a first snap engagement feature. The microfluidic device further includes a modular 3D-printed fiber holder having an outlet and a second snap engagement feature. The first snap engagement feature is configured to engage the second snap engagement feature to removably couple the nozzle to the fiber holder. The outlet of the fiber holder is aligned with the inlet of the nozzle when the first snap engagement feature is coupled to the second snap engagement feature.

Abstract: A microfluidic device for use in a serial crystallography apparatus includes a nozzle having an inlet, an outlet, and a first snap engagement feature. The microfluidic device further includes a fiber holder having an outlet and a second snap engagement feature. The first snap engagement feature is configured to engage the second snap engagement feature to removably couple the nozzle to the fiber holder. The outlet of the fiber holder is aligned with the inlet of the nozzle when the first snap engagement feature is coupled to the second snap engagement feature.

Abstract: A 3D printed hybrid nozzle device combining a microfluidic mixer with a liquid jet injector that addresses the bottleneck of investigating substrate-initiated biological reaction paths employing serial crystallography with XFELs. The hybrid nozzle provides for injecting aqueous protein crystal jets after fast mixing (<5 ms), reaching reaction time points (e.g., about 10 ms to about 150 ms) suitable to resolve enzyme kinetics.

Abstract: Nozzle apparatus for producing a liquid jet are disclosed. An example nozzle apparatus includes: (a) an outer nozzle housing defining a cavity and having an outlet defined at an apex of the cavity, (b) a cylindrical sidewall coupled to a base of the nozzle housing, where the cylindrical sidewall defines a plurality of arch-shaped voids and (c) an inner housing coupled to the outer nozzle housing, where the inner housing has a first end and a second end, where at least the first end of the inner housing is arranged within the cavity of the outer nozzle housing, where the inner housing defines a through-hole that is arranged concentric with the outlet of the outer nozzle housing.

Abstract: A 3D printed hybrid nozzle device combining a microfluidic mixer with a liquid jet injector that addresses the bottleneck of investigating substrate-initiated biological reaction paths employing serial crystallography with XFELs. The hybrid nozzle provides for injecting aqueous protein crystal jets after fast mixing (<5 ms), reaching reaction time points (e.g., about 10 ms to about 150 ms) suitable to resolve enzyme kinetics.

Abstract: Nozzle apparatus for producing a liquid jet are disclosed. An example nozzle apparatus includes: (a) an outer nozzle housing defining a cavity and having an outlet defined at an apex of the cavity, (b) a cylindrical sidewall coupled to a base of the nozzle housing, where the cylindrical sidewall defines a plurality of arch-shaped voids and (c) an inner housing coupled to the outer nozzle housing, where the inner housing has a first end and a second end, where at least the first end of the inner housing is arranged within the cavity of the outer nozzle housing, where the inner housing defines a through-hole that is arranged concentric with the outlet of the outer nozzle housing.