Patents by Inventor Garrett RETTIG

Garrett RETTIG has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • USE OF UNIQUE MOLECULAR IDENTIFIERS FOR IMPROVED ACCURACY OF LONG READ SEQUENCING AND CHARACTERIZATION OF CRISPR EDITING
    Publication number: 20230366020
    Abstract: Described herein is a system and process for long read sequencing using PCR primers with incorporated Unique Molecular Identifiers (UMIs) for generating a single molecule consensus for each starting molecule in the sample population. This method reduces the sequencing error rate by generating a consensus from the individual reads in each UMI group, averaging out sequencing errors to give better confidence in the actual sequence, to allow for increased accuracy of quantifying the precise knock-in event, and reporting perfect HDR integration.
    Type: Application
    Filed: May 12, 2023
    Publication date: November 16, 2023
    Inventors: Mollie Schubert, Thomas Osborne, Matthew McNeill, Garrett Rettig
  • DESIGN OF TWO-PART GUIDE RNAS FOR CRISPRa APPLICATIONS
    Publication number: 20230167463
    Abstract: The present invention pertains to a two-component gRNA for use in a CRISPRa SAM system, wherein the two-component gRNA includes a crRNA and a tracrRNA. The crRNA and tracrRNA form a hybridized, functional gRNA in the CRISPRa SAM system. Kits and methods for using the two-component gRNA for use in a CRISPRa SAM system are also provided.
    Type: Application
    Filed: November 16, 2022
    Publication date: June 1, 2023
    Inventors: Javier Alejandro Gomez Vargas, Ashley Jacobi, Garrett Rettig
  • METHODS FOR NOMINATION OF NUCLEASE ON-/OFF-TARGET EDITING LOCATIONS, DESIGNATED "CTL-seq" (CRISPR Tag Linear-seq)
    Publication number: 20220025365
    Abstract: Described herein are methods for identifying and nominating on- and off-target CRISPR editing sites with improved accuracy and sensitivity.
    Type: Application
    Filed: July 22, 2021
    Publication date: January 27, 2022
    Inventors: Matthew MCNEILL, Rolf TURK, Garrett RETTIG, Ellen BLACK, Yongming SUN, Chris SAILOR, Yu WANG, Keith GUNDERSON, Kyle KINNEY
  • METHODS FOR VARIANT DETECTION
    Publication number: 20210285033
    Abstract: The invention can be used to provide a more efficient and less error-prone method of detecting variants in DNA, such as SNPs and indels. The invention also provides a method for performing inexpensive multiplex assays.
    Type: Application
    Filed: October 30, 2020
    Publication date: September 16, 2021
    Inventors: Caifu CHEN, Joseph DOBOSY, Pak Wah TSANG, Mark Aaron BEHLKE, Scott ROSE, Kristin BELTZ, Garrett RETTIG
  • MODIFIED DOUBLE-STRANDED DONOR TEMPLATES
    Publication number: 20210123035
    Abstract: Described herein are compositions and methods for improving homology directed repair (HDR) efficiency and reducing homology-independent integration following introduction of double strand breaks with engineered nucleases. Additionally, modifications to double stranded DNA donors to improve the donor potency and efficiency of homology directed repair following introduction of double stranded breaks with programmable nucleases.
    Type: Application
    Filed: October 23, 2020
    Publication date: April 29, 2021
    Inventors: Jessica Woodley, Bernice Thommandru, Joseph Dobosy, Mark Behlke, Adam Clore, Garrett Rettig, Beimeng Sun
  • METHODS FOR VARIANT DETECTION
    Publication number: 20170145486
    Abstract: The invention can be used to provide a more efficient and less error-prone method of detecting variants in DNA, such as SNPs and indels. The invention also provides a method for performing inexpensive multiplex assays.
    Type: Application
    Filed: November 25, 2016
    Publication date: May 25, 2017
    Inventors: Caifu CHEN, Joseph DOBOSY, Pak Wah TSANG, Mark Aaron BEHLKE, Scott ROSE, Kristin BELTZ, Garrett RETTIG