Abstract: Condensing devices and methods for providing a concentrated biomolecule condensate to one or more biosensing devices are provided. The concentrated biomolecule condensate is obtained from a fluid sample which potentially contains traces of one or more target biomolecules. The fluid sample is first separated into a filtered liquid and a retentate biomolecule condensate. A novel filtering module is provided for this purpose. The target biomolecules in the retentate biomolecule condensate are further separated from unwanted materials using magnetic beads coated with antibodies of the target biomolecules. The beaded biomolecule condensate obtained thereby is processed to extract constituents of the target biomolecules, thereby obtaining the concentrated biomolecule condensate, which is distributed to a biosensing device.

Abstract: A biosensing device for detecting a presence of target biomolecules is provided, including at least one working electrode having a systematic array of nano-electrode wires projecting vertically from an electrode pad. The nano-electrode wires all have a same shape and size and are distributed non-randomly over the electrode pad. Biosensor probes are attached to the nano-electrode wire, each including a bioreceptor selected to bind with a complementary target biomolecule to create a binding event, and an electrochemical transducer transducing this binding event into an electrical signal conducted by the corresponding nano-electrode wire. A biosensing method using such a device is provided, as well as a fabrication method thereof.

Abstract: A system for rapidly determining the presence or quantity of coliform bacteria in a water sample using the enzymes ?-D-galactosidase and ?-D-glucuronidase. The system includes a first filter means for separating bacteria from the sample and a broth for culturing the bacteria including an inducing agent for inducing enzyme production. A second filter means is used to separate the cultured bacteria from the broth. A lysing agent is exposed to the bacteria on the second filter and incubated with a chemiluminogenic substrate of the enzyme to produce a chemiluminescent product. Light emission is initiated from the second filter means and the emitted light is detected or measured directly from the second filter means using a luminometer adapted to receive the second filter means.

Abstract: A system for rapidly determining the presence or quantity of coliform bacteria in a water sample using the enzymes ?-D-galactosidase and ?-D-glucuronidase. The system includes a first filter means for separating bacteria from the sample and a broth for culturing the bacteria including an inducing agent for inducing enzyme production. A second filter means is used to separate the cultured bacteria from the broth. A lysing agent is exposed to the bacteria on the second filter and incubated with a chemiluminogenic substrate of the enzyme to produce a chemiluminescent product. Light emission is initiated from the second filter means and the emitted light is detected or measured directly from the second filter means using a luminometer adapted to receive the second filter means.