Patents by Inventor Garry Nolan
Garry Nolan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220364160Abstract: Methods include contacting a biological sample with a first probe, where the first probe includes a capture moiety having an oligonucleotide sequence that selectively binds to a RNA in the sample, and a secondary oligonucleotide region that does not bind to the RNA, contacting the sample with a second probe, where the second probe includes a probe binding region that is complementary to, and hybridizes to, a portion of the secondary oligonucleotide region, and includes a reporter moiety, and extending the secondary oligonucleotide region using the second probe as a template to generate an extended secondary oligonucleotide region featuring multiple copies of the reporter moiety, where the reporter moiety includes a plurality of label regions each featuring an oligonucleotide sequence, and one or more of the label regions of the reporter moiety are different from the other label regions of the reporter moiety.Type: ApplicationFiled: May 13, 2022Publication date: November 17, 2022Inventors: Garry Nolan, Julia Kennedy-Darling, Peter J. Miller
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Patent number: 10289802Abstract: Methods and systems for determining progression and other characteristics of microarray expression levels and similar information, alternatively using a network or communications medium or tangible storage medium or logic processor.Type: GrantFiled: March 2, 2012Date of Patent: May 14, 2019Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Peng Qiu, Andrew J. Gentles, Sylvia K. Plevritis, Garry Nolan, Karen Sachs
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Publication number: 20170248604Abstract: The present invention provides an approach for the determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, expression markers and other criteria, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of modulators of cellular activation allows for characterization of pathways and cell populations. Several exemplary diseases that can be analyzed using the invention include AML, MDS, and MPN.Type: ApplicationFiled: October 13, 2016Publication date: August 31, 2017Inventors: Wendy J. Fantl, David B. Rosen, Alessandra Cesano, Santosh K. Putta, Garry Nolan, Aileen Cohen, Erik Evensen
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Patent number: 9500655Abstract: The present invention provides an approach for the determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, expression markers and other criteria, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of modulators of cellular activation allows for characterization of pathways and cell populations. Several exemplary diseases that can be analyzed using the invention include AML, MDS, and MPN.Type: GrantFiled: May 16, 2014Date of Patent: November 22, 2016Assignee: NODALITY, INC.Inventors: Wendy J. Fantl, David B. Rosen, Alessandra Cesano, Santosh K. Putta, Garry Nolan, Aileen Cohen, Erik Evensen
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Publication number: 20150017119Abstract: The present invention provides an approach for the determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, expression markers and other criteria, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of modulators of cellular activation allows for characterization of pathways and cell populations. Several exemplary diseases that can be analyzed using the invention include AML, MDS, and MPN.Type: ApplicationFiled: May 16, 2014Publication date: January 15, 2015Applicant: Nodality, Inc.Inventors: Wendy J. FANTL, David B. ROSEN, Alessandra CESANO, Santosh K. PUTTA, Garry NOLAN, Aileen COHEN, Erik EVENSEN
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Patent number: 8778620Abstract: The present invention provides an approach for the determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, expression markers and other criteria, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of modulators of cellular activation allows for characterization of pathways and cell populations. Several exemplary diseases that can be analyzed using the invention include AML, MDS, and MPN.Type: GrantFiled: May 17, 2012Date of Patent: July 15, 2014Assignee: Nodality, Inc.Inventors: Wendy J. Fantl, David B. Rosen, Alessandra Cesano, Santosh K. Putta, Garry Nolan, Aileen Cohen, Erik Evensen
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Publication number: 20130287790Abstract: Methods, compositions, and kits are provided for the use of JAM-A in diagnosing and treating leukemia. These methods, compositions, and kits find many uses, for example in diagnosing an individual with a leukemia, classifying a leukemia, providing a prognosis to an individual with a leukemia, treating an individual with a leukemia, screening candidate agents for the ability to treat a leukemia, and in basic research to better understand the molecular and cellular basis of leukemia.Type: ApplicationFiled: March 14, 2013Publication date: October 31, 2013Inventors: Faye Yuan-yi Hsu, Garry Nolan, Yury Goltsev
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Publication number: 20130130279Abstract: The present invention provides an approach for the determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, expression markers and other criteria, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of modulators of cellular activation allows for characterization of pathways and cell populations. Several exemplary diseases that can be analyzed using the invention include AML, MDS, and MPN.Type: ApplicationFiled: May 17, 2012Publication date: May 23, 2013Applicant: Nodality, Inc.Inventors: Wendy J. Fantl, David B. Rosen, Alessandra Cesano, Santosh K. Putta, Garry Nolan, Aileen Cohen, Erik Evensen
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Publication number: 20130060775Abstract: Methods and systems for determining progression and other characteristics of microarray expression levels and similar information, alternatively using a network or communications medium or tangible storage medium or logic processor.Type: ApplicationFiled: March 2, 2012Publication date: March 7, 2013Applicant: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Peng Qiu, Andrew J. Gentles, Sylvia K. Plevritis, Garry Nolan, Karen Sachs
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Publication number: 20130024177Abstract: The present invention provides various methods of generating and using models of biological events. The models can be used to classify individuals according to the biological event.Type: ApplicationFiled: March 24, 2011Publication date: January 24, 2013Applicant: Nodality, Inc.Inventor: Garry Nolan
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Patent number: 8309306Abstract: The invention provides methods, kits, devices and compositions for detecting one or more target analytes. In some embodiments, the invention provides binding elements and labeling elements capable of being joined through a plurality of joining elements.Type: GrantFiled: November 12, 2009Date of Patent: November 13, 2012Assignee: Nodality, Inc.Inventors: Garry Nolan, Philip McGarrigle
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Patent number: 8227202Abstract: The present invention provides an approach for the determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, expression markers and other criteria, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of modulators of cellular activation allows for characterization of pathways and cell populations. Several exemplary diseases that can be analyzed using the invention include AML, MDS, and MPN.Type: GrantFiled: July 10, 2009Date of Patent: July 24, 2012Assignee: Nodality, Inc.Inventors: Wendy J. Fantl, David B. Rosen, Alessandra Cesano, Santosh K. Putta, Garry Nolan, Aileen Cohen, Erik Evensen
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Publication number: 20110263457Abstract: We describe herein a cell-based multiplexing technique called detectable cell barcoding (DCB). In DCB, each individual sample is labeled with a different DCB signature that distinguishes each sample by one or both of detected intensity or type of detection characteristic. The samples are then combined and analyzed for a detectable characteristic of interest (e.g., presence of an analyte). By employing multiple distinct DCB labels at varying concentrations, one can perform multiplex analyses on up to hundreds or thousands (or more) of cell samples in a single reaction tube. DCB reduces reagent consumption by factors of 100-fold or more, significantly reduces data acquisition times and allows for stringent control sample analysis.Type: ApplicationFiled: July 1, 2011Publication date: October 27, 2011Inventors: Peter O. Krutzik, Garry Nolan
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Patent number: 8003312Abstract: We describe herein a cell-based multiplexing technique called detectable cell barcoding (DCB). In DCB, each individual sample is labeled with a different DCB signature that distinguishes each sample by one or both of detected intensity or type of detection characteristic. The samples are then combined and analyzed for a detectable characteristic of interest (e.g., presence of an analyte). By employing multiple distinct DCB labels at varying concentrations, one can perform multiplex analyses on up to hundreds or thousands (or more) of cell samples in a single reaction tube. DCB reduces reagent consumption by factors of 100-fold or more, significantly reduces data acquisition times and allows for stringent control sample analysis.Type: GrantFiled: February 14, 2008Date of Patent: August 23, 2011Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Peter O. Krutzik, Garry Nolan
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Publication number: 20100151472Abstract: The invention provides methods, kits, devices and compositions for detecting one or more target analytes. In some embodiments, the invention provides binding elements and labeling elements capable of being joined through a plurality of joining elements.Type: ApplicationFiled: November 12, 2009Publication date: June 17, 2010Applicant: Nodality, Inc.Inventors: Garry Nolan, Phillip McGarrigle
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Publication number: 20100099074Abstract: A collection device and methods for stimulating and stabilizing a biological sample are provided. Aspects of the device include an evacuated sample collection container having stimulating and stabilizing additives contained in separate internal chambers of the container. The internal chambers are arranged to facilitate stimulation upon collection of a sample, followed by stabilization of the sample after a desired period of time in the same sealed container. Also provided are methods for use of the subject device. The device and methods find use in a variety of different applications, including the collection and analysis of clinically relevant samples, such as whole blood and other body fluids, obtained directly from a patient.Type: ApplicationFiled: July 22, 2009Publication date: April 22, 2010Inventors: Garry Nolan, Matthew Hale
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Publication number: 20100009364Abstract: The present invention provides an approach for the determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, expression markers and other criteria, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of modulators of cellular activation allows for characterization of pathways and cell populations. Several exemplary diseases that can be analyzed using the invention include AML, MDS, and MPN.Type: ApplicationFiled: July 10, 2009Publication date: January 14, 2010Applicant: Nodality, Inc.Inventors: Wendy J. Fantl, David B. Rosen, Alessandra Cesano, Santosh K. Putta, Garry Nolan, Aileen Cohen, Erik Evensen
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Publication number: 20080241820Abstract: We describe herein a cell-based multiplexing technique called detectable cell barcoding (DCB). In DCB, each individual sample is labeled with a different DCB signature that distinguishes each sample by one or both of detected intensity or type of detection characteristic. The samples are then combined and analyzed for a detectable characteristic of interest (e.g., presence of an analyte). By employing multiple distinct DCB labels at varying concentrations, one can perform multiplex analyses on up to hundreds or thousands (or more) of cell samples in a single reaction tube. DCB reduces reagent consumption by factors of 100-fold or more, significantly reduces data acquisition times and allows for stringent control sample analysis.Type: ApplicationFiled: February 14, 2008Publication date: October 2, 2008Inventors: Peter O. Krutzik, Garry Nolan
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Publication number: 20070196870Abstract: The invention provides methods and compositions for simultaneously detecting the activation state of a plurality of proteins in single cells using flow cytometry. The invention further provides methods and compositions of screening for bioactive agents capable of coordinately modulating the activity of a plurality of proteins in single cells. The methods and compositions can be used to determine the protein activation profile of a cell for predicting or diagnosing a disease state, and for monitoring treatment of a disease state.Type: ApplicationFiled: January 18, 2007Publication date: August 23, 2007Inventors: Omar Perez, Garry Nolan
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Publication number: 20070196868Abstract: The invention provides methods and compositions for simultaneously detecting the activation state of a plurality of proteins in single cells using flow cytometry. The invention further provides methods and compositions of screening for bioactive agents capable of coordinately modulating the activity of a plurality of proteins in single cells. The methods and compositions can be used to determine the protein activation profile of a cell for predicting or diagnosing a disease state, and for monitoring treatment of a disease state.Type: ApplicationFiled: January 18, 2007Publication date: August 23, 2007Inventors: Omar Perez, Garry Nolan