Abstract: Genetic regulatory elements which effect anoxic induction of a DNA molecule in mammalian cells exposed to anoxia are identified. The genetic regulatory elements, designated mammalian anoxia-responsive elements, when operably linked to a DNA molecule and basal promoter regulate the transcription of the DNA molecule in response to anoxia. The invention relates to recombinant vectors useful for introduction into mammalian cells, and the selective expression in mammalian cells exposed to anoxic conditions. Also provided are methods of using such vectors.

Abstract: An improved assay procedure for quantitatively detecting LDH.sub.k isozyme in human serum is provided which utilizes previously known electrophoretic gel separation procedures combined with an improved staining method wherein the staining solution has a constant temperature of about 37.degree. C. and a pH of about 8.0, and is contacted with the gel matrix for at least 3 hours in the absence of oxygen and light. This improved assay procedure may be used as an adjunct to other procedures for diagnosing the presence of primary cancer, as a post-operative indicator of metastatic cancer and in monitoring the success of cancer therapy.

Abstract: An improved assay procedure for quantitatively detecting LDH.sub.k isozyme in human serum is provided which utilizes previously known electrophoretic gel separation procedures combined with an improved staining method wherein the staining solution has a constant temperature of about 37.degree. C. and a pH of about 8.0, and is contacted with the gel matrix for at least 3 hours in the absence of oxygen and light. This improved assay procedure may be used as an adjunct to other procedures for diagnosing the presence of primary cancer, as a post-operative indicator of metastatic cancer and in monitoring the success of cancer therapy.