Abstract: A fast and accurate reverse-phase high pressure liquid chromatography (“RP-HPLC”) method for detecting amino acids in small volumes (e.g. less than 50 µL) of a biological sample, such as plasma. An assay for the simultaneous determination of ammonium and primary amino acids using RP-HPLC in samples such as plasma. A method for calculating intercellular volumes from a cell lysate to which a known volume and concentration of a non-naturally occurring amino acid is added.

Abstract: The present invention provides compositions and methods of treating hyperphenylalaninemia (e.g., phenylketonuria) in a subject in need thereof comprising administering to the subject an effective amount of a phenylalanine dehydrogenase (PheDH) polypeptide. The present invention also provides pharmaceutical formulations comprising PheDH for lowering the phenylalanine concentration in the subject (e.g., in the intestines and/or blood).

Abstract: The present invention provides compositions and methods of treating hyperphenylalaninemia (e.g., phenylketonuria) in a subject in need thereof comprising administering to the subject an effective amount of a phenylalanine dehydrogenase (PheDH) polypeptide. The present invention also provides pharmaceutical formulations comprising PheDH for lowering the phenylalanine concentration in the subject (e.g., in the intestines and/or blood).

Abstract: The present disclosure relates to a biosensor capable of measuring the total concentration of one or a plurality of ammonia or ammonium ions with the use of indophenol reagents in the presence of an ionomer. In some embodiments, the biosensor comprises a perfluorinated membrane that comprises an ionomer in contact with an alkali buffer in a vessel configured to receive a sample, such as whole blood. The disclosure also relates to a method of detecting or quantifying the ammonia or ammonium ion concentration in whole blood in a point of care bio sensor without reliance on gas chromatography or any measurement that takes more than about twenty minutes.

Abstract: The present disclosure relates to a biosensor capable of measuring the total concentration of one or a plurality of ammonia or ammonium ions with the use of indophenol reagents in the presence of an ionomer. In some embodiments, the biosensor comprises a perflurinated membrane that comprises an ionomer in contact with an alkali buffer in a vessel configured to receive a sample, such as whole blood. The disclosure also relates to a method of detecting or quantifying the ammonia or ammonium ion concentration in whole blood in a point of care bio sensor without reliance on gas chromatography or any measurement that takes more than about twenty minutes.

Abstract: The present invention relates to a biosensor capable of measuring the total concentration of one or a plurality of amino acids with the use of a reagentless system comprising an electrode modified by hydrogel that comprises at least one enzyme that oxidizes at least one substrate that is at least one amino acid. In some embodiments, the biosensor comprises a hydrogel comprising alginate. In some embodiments, the biosensor comprises use of a thermophilic bacterial metabolic enzyme immobilized or attached to the hydrogel.

Abstract: The present application provides an electromagnetic coupling interface and a method for managing an electromagnetic coupling capability relative to a particular charge location with an electromagnetic coupling interface. The electromagnetic coupling interface includes a carrier substrate including two or more moveably coupled segments, the carrier substrate having at least two use positions, where in each use position the two or more moveably coupled segments have a different spatial arrangement. The electromagnetic coupling further includes two or more sets of conductors, where each set of conductors is associated with a respective one of the two or more moveably coupled segments of the carrier substrate and is associated with one or more coils in each of the at least two use positions.

Abstract: The present disclosure relates to a biosensor capable of measuring the total concentration of one or a plurality of ammonia or ammonium ions with the use of indophenol reagents in the presence of an ionomer. In some embodiments, the biosensor comprises a perflurinated membrane that comprises an ionomer in contact with an alkali buffer in a vessel configured to receive a sample, such as whole blood. The disclosure also relates to a method of detecting or quantifying the ammonia or ammonium ion concentration in whole blood in a point of care bio sensor without reliance on gas chromatography or any measurement that takes more than about twenty minutes.

Abstract: The present disclosure relates to a biosensor capable of measuring the total concentration of one or a plurality of ammonia or ammonium ions with the use of indophenol reagents in the presence of an ionomer. In some embodiments, the biosensor comprises a perflurinated membrane that comprises an ionomer in contact with an alkali buffer in a vessel configured to receive a sample, such as whole blood. The disclosure also relates to a method of detecting or quantifying the ammonia or ammonium ion concentration in whole blood in a point of care bio sensor without reliance on gas chromatography or any measurement that takes more than about twenty minutes.

Abstract: The present disclosure relates to a bio-sensor capable of measuring the total concentration of one or a plurality of ammonia or ammonium ions with the use of indophenol reagents in the presence of an ionomer. In some embodiments, the biosensor comprises a perflurinated membrane that comprises an ionomer in contact with an alkaline buffer in a vessel configured to receive a sample, such as whole blood. The disclosure also relates to a method of detecting or quantifying the ammonia or ammonium ion concentration in whole blood in a point of care biosensor without reliance on gas chromatography or any measurement that takes more than about twenty minutes.

Abstract: The present application provides an electromagnetic coupling interface and a method for managing an electromagnetic coupling capability relative to a particular charge location with an electromagnetic coupling interface. The electromagnetic coupling interface includes a carrier substrate including two or more moveably coupled segments, the carrier substrate having at least two use positions, where in each use position the two or more moveably coupled segments have a different spatial arrangement. The electromagnetic coupling further includes two or more sets of conductors, where each set of conductors is associated with a respective one of the two or more moveably coupled segments of the carrier substrate and is associated with one or more coils in each of the at least two use positions.

Abstract: The present disclosure relates to a biosensor capable of measuring the total concentration of one or a plurality of amino acids with the use of a reaction surface comprising one or a plurality of metabolic enzymes or functional fragments thereof, but wherein the reaction surface does not comprise an electrode or electrically conductive support. In some embodiments, the biosensor comprises use of a thermophilic bacterial metabolic enzyme immobilized or attached to the reaction surface.

Abstract: The present invention provides compositions and methods of treating hyperphenylalaninemia (e.g., phenylketonuria) in a subject in need thereof comprising administering to the subject an effective amount of a phenylalanine dehydrogenase (PheDH) polypeptide. The present invention also provides pharmaceutical formulations comprising PheDH for lowering the phenylalanine concentration in the subject (e.g., in the intestines and/or blood).

Abstract: The present disclosure relates to a bio-sensor capable of measuring the total concentration of one or a plurality of ammonia or ammonium ions with the use of indophenol reagents in the presence of an ionomer. In some embodiments, the biosensor comprises a perflurinated membrane that comprises an ionomer in contact with an alkaline buffer in a vessel configured to receive a sample, such as whole blood. The disclosure also relates to a method of detecting or quantifying the ammonia or ammonium ion concentration in whole blood in a point of care biosensor without reliance on gas chromatography or any measurement that takes more than about twenty minutes.

Abstract: The present invention relates to a biosensor capable of measuring the total concentration of one or a plurality of amino acids with the use of a reagentless system comprising an electrode modified by hydrogel that comprises at least one enzyme that oxidizes at least one substrate that is at least one amino acid. In some embodiments, the biosensor comprises a hydrogel comprising alginate. In some embodiments, the biosensor comprises use of a thermophilic bacterial metabolic enzyme immobilized or attached to the hydrogel.

Abstract: The present invention concerns the ?-glutamylcysteine treatment to reduce oxidative damage caused by ischemia/reperfusion events such as pulmonary hypertension, transplant, cardiac bypass and other surgeries, and trauma. In specific embodiments, preservation methods and apparatuses for preserving tissue for transplantation purposes is provided.

Abstract: A method of making a lid for a casket comprises providing tooling configured to produce a one-piece, unitary casket lid having a crown, a pie, a rim and a header, providing settable material from which to mold the lid, molding the settable material with the tooling, permitting the settable material to set thereby producing a one-piece, unitary casket lid having a crown, a pie, a rim and a header, and adhesively applying a wood veneer sheet to at least a portion of the lid. The veneer sheet can be applied with either a membrane press or a profile wrapping machine.

Abstract: A casket comprises a shell having a pair of opposed side walls, a pair of opposed end walls, and an upwardly facing flange, a lid closeable upon the edge of the shell, and a cover securable to the shell and concealing the flange when the lid is open, the cover being at least semi-rigid, the cover having a one-piece right angle section and a straight section.

Abstract: A funerary article comprises a container having a pair of side wide walls, a pair of end walls and a bottom wall. The container is adapted to contain remains of a deceased. A lid is closeable upon the container. The container has external surfaces defining an exterior of the container. A light source is mounted to the container exterior. The funerary article can be a casket or a cremation urn.

Abstract: A casket comprises a shell having a pair of opposed side walls and a pair of opposed end walls, the walls defining an upper edge of the shell, a lid closeable upon the edge of the shell and a cover securable to the shell and concealing the edge when the lid is open, the cover being at least semi-rigid.