Abstract: Production of polyhydroxyalkanoates (PHAs) is performed by delivering substrates such as methane in gas phase during a high solids fermentation. Microorganisms are grown under balanced conditions, then gas phase substrates are delivered under unbalanced conditions to produce PHA granules inside the cells. The cells containing these granules are lysed and the bioplastic powder recovered. The balanced phase growth may occur in submerged liquid cultures or attached as biofilms to a surface.

Abstract: The present invention is directed to a method for purifying an underground water supply containing halogenated aliphatic compounds by in-situ biodegradation of said compounds, comprising injecting water into an aquifer zone, the injected water containing an electron donor capable of being utilized by an indigenous population of microorganisms contained in the groundwater supply as a primary growth substrate, and an electron acceptor for oxidation of the primary substrate, by separate addition of electron donor and electron acceptor temporally or spatially to stimulate growth of the microorganisms and to distribute them throughout the aquifer zone to cause in-situ degradation of the halogenated aliphatic compounds by metabolic activity of the microorganisms.