Abstract: The present invention provides for a method or methods of targeted genetic recombination or mutagenesis in a host cell or organism, and compositions useful for carrying out the method. The targeting method of the present invention exploits endogenous cellular mechanisms for homologous recombination and repair of double stranded breaks in genetic material. The present invention provides numerous improvements over previous mutagenesis methods, such advantages include that the method is generally applicable to a wide variety of organisms, the method is targeted so that the disadvantages associated with random insertion of DNA into host genetic material are eliminated, and certain embodiments require relatively little manipulation of the host genetic material for success. Additionally, it provides a method that produces organisms with specific gene modifications in a short period of time.

Abstract: The present invention provides for a method or methods of targeted genetic recombination or mutagenesis in a host cell or organism, and compositions useful for carrying out the method. The targeting method of the present invention exploits endogenous cellular mechanisms for homologous recombination and repair of double stranded breaks in genetic material. The present invention provides numerous improvements over previous mutagenesis methods, such advantages include that the method is generally applicable to a wide variety of organisms, the method is targeted so that the disadvantages associated with random insertion of DNA into host genetic material are eliminated, and certain embodiments require relatively little manipulation of the host genetic material for success. Additionally, it provides a method that produces organisms with specific gene modifications in a short period of time.

Abstract: The various embodiments disclosed and pictured herein illustrate a liquid vacuum apparatus and method that is easy to operate, provides increased safety for operators, mitigates the risk of operator exposure to the liquid, and mitigates the risk of liquid spillage during and after the liquid is transported. As described herein, the liquid vacuum apparatus may be employed either with or without the connector, piping, and end piece; and if an end piece is used, it may take many different forms depending on the particular application.

Abstract: The present invention provides for a method or methods of targeted genetic recombination or mutagenesis in a host cell or organism, and compositions useful for carrying out the method. The targeting method of the present invention exploits endogenous cellular mechanisms for homologous recombination and repair of double stranded breaks in genetic material. The present invention provides numerous improvements over previous mutagenesis methods, such advantages include that the method is generally applicable to a wide variety of organisms, the method is targeted so that the disadvantages associated with random insertion of DNA into host genetic material are eliminated, and certain embodiments require relatively little manipulation of the host genetic material for success. Additionally, it provides a method that produces organisms with specific gene modifications in a short period of time.

Abstract: A portable sign holding apparatus for use in single and double-hung windows generally comprising a central housing unit which includes horizontal mounting means for engaging the interior of window jambs, vertical mounting means for engaging the window sill and sash, and sign support means capable of suspending a sign in perpendicular relationship to the face of a building. In use, the horizontal and vertical mounting means engage the surfaces of windows of different sizes via telescopic adjustability to prevent lateral and vertical movement of the apparatus, respectively, within the window frame. Movement of the apparatus and support of the weight of the sign are further provided by a bracket disposed between the sign support means and the exterior wall surface of the building. In combination, the bracket, and vertical and horizontal mounting means provide a highly stable sign suspension device which may be quickly and easily installed without any screws or other invasive means.