Abstract: Examples of molded substrates are described herein. In some examples, a molded substrate may support integrated circuitry. In some examples, the molded substrate and the integrated circuitry are included in a circuitry package for a replaceable print component. In some examples, the molded substrate is relatively flat. In some examples, molding remnants may be on the molded substrate.

Abstract: Examples of molded substrates are described herein. In some examples, a molded substrate may support integrated circuitry. In some examples, the molded substrate and the integrated circuitry are included in a circuitry package for a replaceable print component. In some examples, the molded substrate is relatively flat. In some examples, molding remnants may be on the molded substrate.

Abstract: A roof mounted ventilation assembly having an integrated supply and relief fan assembly. The assembly is drum shaped with a central portion circumferentially defined by centrifugal blades that extend between top and bottom plates. Both top and bottom plates have a central opening, respectively, and axial supply fan blades are disposed within the central opening of the top plate. A supply duct extends through the central portion, fluidly coupling the central openings. The supply duct fluidly communicates a roof-mounted air intake to a supply discharge plenum operatively associated with an interior space. An inlet collar is fluidly couples the remainder of the central portion not closed off by the supply duct to the interior space, wherein relief air in this remainder of the central portion is urged by the centrifugal blades out exhaust ducts to the external environment in a direction transverse relative to axial supply air.

Abstract: A roof mounted ventilation assembly having fluidly coupled supply and relief fans is provided. One drive unit assembly may be operatively associated with both fans. A rotatably and vertically movable duct fluidly couples a supply discharge plenum to a roof-mounted air intake hood so that the former may move vertically and rotatably relative to the latter, thereby the present invention can selectively pressurize the spaced inhabited by the supply discharge plenum through a positive supply to relief air volume ratio.

Abstract: A roof mounted ventilation assembly having fluidly coupled supply and relief fans is provided. One drive unit assembly may be operatively associated with both fans. A rotatably and vertically movable duct fluidly couples a supply discharge plenum to a roof-mounted air intake hood so that the former may move vertically and rotatably relative to the latter, thereby the present invention can selectively pressurize the spaced inhabited by the supply discharge plenum through a positive supply to relief air volume ratio.

Abstract: The present invention provides polypeptides having a nucleotide polymerase activity and method of enhancing polymerase activity. The polypeptides of the present invention may possess both a DNA-dependent DNA polymerase activity and an RNA-dependent DNA polymerase activity, i.e., a reverse transcriptase activity. The polypeptides of the present invention may be used in any application including, but not limited to, DNA sequencing reactions, amplification reactions, cDNA synthesis reactions, and combined cDNA synthesis and amplification reactions, e.g., RT-PCR.

Abstract: The present invention relates to nucleic acid inhibitors, compositions and method for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses nucleic acids having affinity for polypeptides with polymerase activity for use in such synthesis, amplification or sequencing reactions. The nucleic acid inhibitors are capable of inhibiting nonspecific nucleic acid synthesis under certain conditions (e.g., at ambient temperatures). Thus, in a preferred aspect, the invention relates to “hot start” synthesis of nucleic acid molecules. Accordingly, the invention prevents, reduces or substantially reduces nonspecific nucleic acid synthesis. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the nucleic acid inhibitors or compositions of the invention.

Abstract: The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.

Abstract: An oral care implement includes a head and a plurality of cleaning elements for enhanced cleaning of the teeth and soft tissue of the oral cavity. One tooth cleaning element has a plurality of bristles adapted to clean teeth. Another cleaning element is connected to the head and has structure defining a channel. The channel is configured to direct fluid in contact with the head toward an edge of the head when the implement is moved. The head also defines a reservoir that is configured to receive a dentifrice therein.

Abstract: The present invention provides polypeptides having a nucleotide polymerase activity and method of enhancing polymerase activity. The polypeptides of the present invention may possess both a DNA-dependent DNA polymerase activity and an RNA-dependent DNA polymerase activity, i.e., a reverse transcriptase activity. The polypeptides of the present invention may be used in any application including, but not limited to, DNA sequencing reactions, amplification reactions, cDNA synthesis reactions, and combined cDNA synthesis and amplification reactions, e.g., RT-PCR.

Abstract: A reflux trap for use in a plasma generator and delivery system to prevent back flow of fluid and debris from a patient during a surgical procedure including a trap housing having a proximal diffuser chamber and a distal deflection chamber with a collection area separated by a baffle disposed within the trap housing including a diffuser member disposed in the proximal diffuser chamber to diffuse gas through a gas flow aperture to the surgical site on the patient and a deflection member disposed in the distal deflection chamber to deflect fluid and debris away from the gas flow aperture to the collection area to prevent fluid and debris from migrating into the proximal diffuser chamber to isolate the plasma generator against back flow of fluid and debris from the patient.

Abstract: A reflux trap for use in a plasma generator and delivery system to prevent back flow of fluids and debris from a patient to the plasma generator during a surgical procedure comprising a trap housing including a proximal diffuser chamber and a distal deflection chamber having a collection area separated by a baffle disposed within the trap housing having at least one gas flow aperture formed therethrough, a proximal inlet port and a distal outlet port formed on opposite end portions of the trap housing to feed gas to and from the trap housing through the proximal diffuser chamber, gas flow aperture and the distal deflection chamber, the baffle further includes a diffuser member disposed in the proximal diffuser chamber such that gas fed into the proximal diffuser chamber through the proximal inlet port impigns thereon and diffused through the gas flow aperture into the distal deflection chamber and through the distal outlet port to the surgical site on the patient and a deflection member disposed in the distal

Abstract: An oral care implement includes a head and a plurality of cleaning elements for enhanced cleaning of the teeth and soft tissue of the oral cavity. One tooth cleaning element has a plurality of bristles adapted to clean teeth. Another cleaning element is connected to the head and has structure defining a channel. The channel is configured to direct fluid in contact with the head toward an edge of the head when the implement is moved. The head also defines a reservoir that is configured to receive a dentifrice therein.

Abstract: The invention relates to a gene which encodes reverse transcriptase having DNA polymerase activity and substantially no RNase H activity. The invention also relates to vectors containing the gene and hosts transformed with the vectors of the invention. The invention also relates to a method of producing reverse transcriptase having DNA polymerase activity and substantially no RNase H activity by expressing the reverse transcriptase genes of the present invention in a host. The invention also relates to a method of producing cDNA from mRNA using the reverse transcriptase of the invention. The invention also relates to a kit for the preparation of cDNA from mRNA comprising the reverse transcriptase of the invention.

Abstract: The present invention is generally related to compositions and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to compositions comprising mixtures of polypeptides having reverse transcriptase (RT) activity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these compositions or polypeptides, particularly at temperatures above about 55° C. The invention also relates to nucleic acid molecules produced by these methods, to vectors and host cells comprising these nucleic acid molecules, and to the use of such nucleic acid molecules to produce desired polypeptides.

Abstract: The present invention relates to isolated nucleic acid sequences encoding CEL II endonuclease and vectors and host cells for producing a protein encoded thereby.

Abstract: The invention is related generally to methods of amplifying or synthesizing or producing nucleic acid molecules using Translesion DNA polymerases. In particular, the invention relates to methods of introducing a random mutation into a nucleic acid and encoded polypeptide using Translesion DNA polymerases. The invention also relates to methods of introducing a modified nucleotide into a nucleic acid using Translesion DNA polymerases. The invention also relates to mutagenized and modified nucleic acid molecules and proteins produced by these methods, and to fragments or derivatives thereof. The invention also relates to vectors and host cells comprising mutagenized nucleic acid molecules, fragments, or derivatives. The invention also relates to the use of mutagenized nucleic acid molecules to produce desired polypeptides and uses of modified nucleic acid molecules to analyze samples. The invention also relates to kits or compositions or compounds for use in the invention or for carrying out the invention.

Abstract: The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.

Abstract: The present invention provides polypeptides having a nucleotide polymerase activity and method of enhancing polymerase activity. The polypeptides of the present invention may posses both a DNA-dependent DNA polymerase activity and an RNA-dependent DNA polymerase activity, i.e., a reverse transcriptase activity. The polypeptides of the present invention may be used in any application including, but not limited to, DNA sequencing reactions, amplification reactions, cDNA synthesis reactions, and combined cDNA synthesis and amplification reactions, e.g., RT-PCR.