Patents by Inventor Gary Lesnicki

Gary Lesnicki has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Fermentation process for antibody production
    Patent number: 11597758
    Abstract: A feedback control mechanism for a fermentation of yeast cells to make recombinant proteins uses a respiratory quotient measurement which adjusts the levels of oxygenation and/or fermentable sugar feed. The feedback control mechanism permits well controlled cultures that produce good amounts of product while avoiding toxic accumulation of ethanol. Additionally, recombinant proteins so produced have excellent qualitative properties, such as excellent homogeneity and proper inter-subunit assembly.
    Type: Grant
    Filed: January 25, 2019
    Date of Patent: March 7, 2023
    Assignee: H. LUNDBECK A/S
    Inventors: Gary Lesnicki, Patricia Dianne McNeill, Franz Hartner, Mark Young
  • yeast cultures comprising reduced antibody-associated variants
    Patent number: 11447540
    Abstract: Methods for producing heterologous proteins are disclosed. In particular, the present disclosure provides improved methods of producing desired proteins, including multi-subunit proteins such as antibodies, with a higher yield and improved purity. In exemplary embodiments, the transformed cells are a yeast, e.g., methylotrophic yeast such as Pichia pastoris.
    Type: Grant
    Filed: October 24, 2018
    Date of Patent: September 20, 2022
    Assignee: H. LUNDBECK A/S
    Inventors: Gary Lesnicki, Patricia Dianne McNeill, Franz Hartner, Mark Young
  • High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as
    Patent number: 11214610
    Abstract: Methods for producing heterologous multi-subunit proteins in transformed cells are disclosed. In particular, the present disclosure provides improved methods of producing multi-subunit proteins, including antibodies and other multi-subunit proteins, which may or may not be secreted, with a higher yield and decreased production of undesired side-products. In exemplary embodiments, the transformed cells are a yeast, e.g., methylotrophic yeast such as Pichia pastoris.
    Type: Grant
    Filed: May 8, 2012
    Date of Patent: January 4, 2022
    Assignee: H. LUNDBECK A/S
    Inventors: Patricia Dianne McNeill, Leon F. Garcia-Martinez, Nicole Janson, Gary Lesnicki, Pei Qi, John A. Latham
  • TEMPERATURE SHIFT FOR HIGH YIELD EXPRESSION OF POLYPEPTIDES IN YEAST AND OTHER TRANSFORMED CELLS
    Publication number: 20190390239
    Abstract: Methods for producing heterologous proteins are disclosed. In particular, the present disclosure provides improved methods of producing desired proteins, including multi-subunit proteins such as antibodies, with a higher yield and improved purity. In exemplary embodiments, the transformed cells are a yeast, e.g., methylotrophic yeast such as Pichia pastoris.
    Type: Application
    Filed: February 11, 2019
    Publication date: December 26, 2019
    Inventors: Gary Lesnicki, Mark Young
  • FERMENTATION PROCESS FOR ANTIBODY PRODUCTION
    Publication number: 20190211080
    Abstract: A feedback control mechanism for a fermentation of yeast cells to make recombinant proteins uses a respiratory quotient measurement which adjusts the levels of oxygenation and/or fermentable sugar feed. The feedback control mechanism permits well controlled cultures that produce good amounts of product while avoiding toxic accumulation of ethanol. Additionally, recombinant proteins so produced have excellent qualitative properties, such as excellent homogeneity and proper inter-subunit assembly.
    Type: Application
    Filed: January 25, 2019
    Publication date: July 11, 2019
    Inventors: Gary Lesnicki, Patricia Dianne McNeill, Franz Hartner, Mark Young
  • TEMPERATURE SHIFT FOR HIGH YIELD EXPRESSION OF POLYPEPTIDES IN YEAST AND OTHER TRANSFORMED CELLS
    Publication number: 20190119360
    Abstract: Methods for producing heterologous proteins arc disclosed. In particular, the present disclosure provides improved methods of producing desired proteins, including multi-subunit proteins such as antibodies, with a higher yield and improved purity. In exemplary embodiments, the transformed cells are a yeast, e.g., methylotrophic yeast such as Pichia pastoris.
    Type: Application
    Filed: October 24, 2018
    Publication date: April 25, 2019
    Inventors: GARY LESNICKI, PATRICIA DIANNE MCNEILL, FRANZ HARTNER, MARK YOUNG
  • Temperature shift for high yield expression of polypeptides in yeast and other transformed cells
    Patent number: 10202630
    Abstract: Methods for producing heterologous proteins are disclosed. In particular, the present disclosure provides improved methods of producing desired proteins, including multi-subunit proteins such as antibodies, with a higher yield and improved purity. In exemplary embodiments, the transformed cells are a yeast, e.g., methylotrophic yeast such as Pichia pastoris.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: February 12, 2019
    Assignee: ALDERBIO HOLDINGS LLC
    Inventors: Gary Lesnicki, Mark Young
  • Fermentation process for antibody production
    Patent number: 10189892
    Abstract: A feedback control mechanism for a fermentation of yeast cells to make recombinant proteins uses a respiratory quotient measurement which adjusts the levels of oxygenation and/or fermentable sugar feed. The feedback control mechanism permits well controlled cultures that produce good amounts of product while avoiding toxic accumulation of ethanol. Additionally, recombinant proteins so produced have excellent qualitative properties, such as excellent homogeneity and proper inter-subunit assembly.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: January 29, 2019
    Assignee: ALDERBIO HOLDINGS LLC
    Inventors: Gary Lesnicki, Patricia Dianne McNeill, Franz Hartner, Mark Young
  • Temperature shift for high yield expression of polypeptides in yeast and other transformed cells
    Patent number: 10138294
    Abstract: Methods for producing heterologous proteins are disclosed. In particular, the present disclosure provides improved methods of producing desired proteins, including multi-subunit proteins such as antibodies, with a higher yield and improved purity. In exemplary embodiments, the transformed cells are a yeast, e.g., methylotrophic yeast such as Pichia pastoris.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: November 27, 2018
    Assignee: ALDERBIO HOLDINGS LLC
    Inventors: Gary Lesnicki, Patricia Dianne McNeill, Franz Hartner, Mark Young
  • TEMPERATURE SHIFT FOR HIGH YIELD EXPRESSION OF POLYPEPTIDES IN YEAST AND OTHER TRANSFORMED CELLS
    Publication number: 20160039911
    Abstract: Methods for producing heterologous proteins are disclosed. In particular, the present disclosure provides improved methods of producing desired proteins, including multi-subunit proteins such as antibodies, with a higher yield and improved purity. In exemplary embodiments, the transformed cells are a yeast, e.g., methylotrophic yeast such as Pichia pastoris.
    Type: Application
    Filed: March 17, 2014
    Publication date: February 11, 2016
    Inventors: Gary Lesnicki, Patricia Dianne McNeill, Franz Hartner, Mark Young
  • FERMENTATION PROCESS FOR ANTIBODY PRODUCTION
    Publication number: 20160031970
    Abstract: A feedback control mechanism for a fermentation of yeast cells to make recombinant proteins uses a respiratory quotient measurement which adjusts the levels of oxygenation and/or fermentable sugar feed. The feedback control mechanism permits well controlled cultures that produce good amounts of product while avoiding toxic accumulation of ethanol. Additionally, recombinant proteins so produced have excellent qualitative properties, such as excellent homogeneity and proper inter-subunit assembly.
    Type: Application
    Filed: March 17, 2014
    Publication date: February 4, 2016
    Applicant: ALDERBIO HOLDINGS LLC
    Inventors: Gary Lesnicki, Patricia Dianne McNeill, Franz Hartner, Mark Young
  • TEMPERATURE SHIFT FOR HIGH YIELD EXPRESSION OF POLYPEPTIDES IN YEAST AND OTHER TRANSFORMED CELLS
    Publication number: 20140287459
    Abstract: Methods for producing heterologous proteins are disclosed. In particular, the present disclosure provides improved methods of producing desired proteins, including multi-subunit proteins such as antibodies, with a higher yield and improved purity. In exemplary embodiments, the transformed cells are a yeast, e.g., methylotrophic yeast such as Pichia pastoris.
    Type: Application
    Filed: March 17, 2014
    Publication date: September 25, 2014
    Applicant: ALDERBIO HOLDINGS LLC
    Inventors: Gary Lesnicki, Mark Young
  • HIGH-PURITY PRODUCTION OF MULTI-SUBUNIT PROTEINS SUCH AS ANTIBODIES IN TRANSFORMED MICROBES SUCH AS PICHIA PASTORIS
    Publication number: 20120277408
    Abstract: Methods for producing heterologous multi-subunit proteins in transformed cells are disclosed. In particular, the present disclosure provides improved methods of producing multi-subunit proteins, including antibodies and other multi-subunit proteins, which may or may not be secreted, with a higher yield and decreased production of undesired side-products. In exemplary embodiments, the transformed cells are a yeast, e.g., methylotrophic yeast such as Pichia pastoris.
    Type: Application
    Filed: May 8, 2012
    Publication date: November 1, 2012
    Applicant: Alderbio Holdings LLC
    Inventors: Patricia Dianne McNeill, Leon F. Garcia-Martinez, Nicole Janson, Gary Lesnicki, Pei Qi, John A. Latham