Abstract: The present disclosure encompasses engineered meganucleases that bind and cleave recognition sequences within a dystrophin gene. The present disclosure also encompasses methods of using such engineered meganucleases to make genetically modified cells. Further, the disclosure encompasses pharmaceutical compositions comprising engineered meganuclease proteins, or polynucleotides encoding engineered meganucleases of the disclosure, and the use of such compositions for the modification of a dystrophin gene in a subject, or for treatment of Duchenne Muscular Dystrophy.

Abstract: The present disclosure encompasses engineered meganucleases that bind and cleave recognition sequences within a dystrophin gene. The present disclosure also encompasses methods of using such engineered meganucleases to make genetically modified cells. Further, the disclosure encompasses pharmaceutical compositions comprising engineered meganuclease proteins, or polynucleotides encoding engineered meganucleases of the disclosure, and the use of such compositions for the modification of a dystrophin gene in a subject, or for treatment of Duchenne Muscular Dystrophy.

Abstract: The present disclosure encompasses engineered meganucleases that bind and cleave recognition sequences within a dystrophin gene. The present disclosure also encompasses methods of using such engineered meganucleases to make genetically modified cells. Further, the disclosure encompasses pharmaceutical compositions comprising engineered meganuclease proteins, or polynucleotides encoding engineered meganucleases of the disclosure, and the use of such compositions for the modification of a dystrophin gene in a subject, or for treatment of Duchenne Muscular Dystrophy.

Abstract: The present disclosure encompasses engineered meganucleases that bind and cleave recognition sequences within a dystrophin gene. The present disclosure also encompasses methods of using such engineered meganucleases to make genetically modified cells. Further, the disclosure encompasses pharmaceutical compositions comprising engineered meganuclease proteins, or polynucleotides encoding engineered meganucleases of the disclosure, and the use of such compositions for the modification of a dystrophin gene in a subject, or for treatment of Duchenne Muscular Dystrophy.

Abstract: Disclosed are engineered nucleases that bind and cleave a recognition sequence within a hydroxyacid oxidase 1 (HAO1) gene. The present invention also encompasses methods of using such engineered nucleases to make genetically-modified cells. Further, the invention encompasses pharmaceutical compositions comprising engineered nuclease proteins or nucleic acids encoding engineered nucleases of the invention, and the use of such compositions for treatment of primary hyperoxaluria type I.

Abstract: A thin flexible conformable electrochemical cell for powering a wearable electrical device comprising an inner electrode having an active electrode face of one charge and an outer electrode having an active electrode face of the opposite charge separated by a separator, wherein said separator comprises an electrolyte layer as a single continuous layer folded around the inner electrode, and wherein the cell has a single continuous flexible coating material folded around the separator and the inner electrode so as to offer protection for the cell, and wherein the coating material is sealable so as define access to the cell for electrode contacts for connection with the electrical device, and so as to offer avoidance of the cell short circuiting in use. Also provided are methods for cell preparation.

Abstract: A laser ablation system includes a laser configured to irradiate or sublimate a portion of a thin-film coating located on an electronic device, a controller for controlling the laser, and an optical system comprising one or more optical sensors configured to detect the electronic component and a location of the portion of the thin-film coating to be irradiated or sublimated.

Abstract: A thin flexible conformable electrochemical cell for powering a wearable electrical device comprising an inner electrode having an active electrode face of one charge and an outer electrode having an active electrode face of the opposite charge separated by a separator, wherein said separator comprises an electrolyte layer as a single continuous layer folded around the inner electrode, and wherein the cell has a single continuous flexible coating material folded around the separator and the inner electrode so as to offer protection for the cell, and wherein the coating material is sealable so as define access to the cell for electrode contacts for connection with the electrical device, and so as to offer avoidance of the cell short circuiting in use. Also provided are methods for cell preparation.

Abstract: The invention relates to devices incorporating thin, lightweight electrochemical cells and their method of manufacture, whereby a thin flexible pouch-type cell (1) comprises at least one pair of overlying electrode layers separated from one another by an intermediate electrolyte layer (13), the cell exterior being defined by first and second laminated sheets (3, 9) sealed together, wherein each laminated sheet (3, 9) has an outermost layer (3a, 9a) forming a respective external face of the cell (1) and a coextensive, innermost, conductive layer (3b, 9b) that acts as a current collector layer (3b, 9b) and which supports an electrode layer (5, 11), although the conductive layer may also itself act as the active electrode layer.

Abstract: A method, apparatus and computer program product that allows for maintaining correct states of all sub-components in a state machine, even as sub-components leave the state machine and later rejoin in some previous state. Preferably, this is achieved without requiring the system to remember the states of all sub-components or a log of every event that was fed into the state machine. Thus, the technique does not require any knowledge of the previous state of the sub-components nor the need to preserve a complete log of events that were fed into the state machine. The state machine may be used to enhance the operation of a technological process, such as a workload management environment.

Abstract: A method, apparatus and computer program product for improved load balancing provides for the grouping under a same workload of both application instances in an application tier, and data sharing members in a data tier. This grouping enables a workload manager to make recommendations (to load balancer appliances) about how to distribute workload connections, e.g., based on metrics gathered from both the application and data tiers. In this approach, both applications and data sources are grouped into a workload grouping, and health, status and capacity information about both of these tiers (application and data) is then used to determine an overall distribution policy for the workload. These different tiers can reside on the same or different operating system environments.

Abstract: A method, apparatus and computer program product for improved load balancing provides for the grouping under a same workload of both application instances in an application tier, and data sharing members in a data tier. This grouping enables a workload manager to make recommendations (to load balancer appliances) about how to distribute workload connections, e.g., based on metrics gathered from both the application and data tiers. In this approach, both applications and data sources are grouped into a workload grouping, and health, status and capacity information about both of these tiers (application and data) is then used to determine an overall distribution policy for the workload. These different tiers can reside on the same or different operating system environments.

Abstract: A periscope includes an object window 4, a first reflector 5, a second reflector 6; and an intermediate port between the first reflector 5 and the second reflector 6. The first reflector 5 is arranged to reflect light from the object window 4 towards the second reflector 6 through the intermediate port. The second reflector 6 is selectively movable between a first position of use in which the second reflector 6 reflects light from the first reflector 5 towards a viewing position and a second position of use in which the second reflector 6 closes the intermediate port.

Abstract: A method that provides for the grouping under a same workload of both application instances in an application tier, and data sharing members in a data tier. This grouping enables a workload manager to make recommendations (to load balancer appliances) about how to distribute workload connections, e.g., based on metrics gathered from both the application and data tiers. In this approach, both applications and data sources are grouped into a workload grouping, and health, status and capacity information about both of these tiers (application and data) is then used to determine an overall distribution policy for the workload. These different tiers can reside on the same or different operating system environments.

Abstract: A method that provides for the grouping under a same workload of both application instances in an application tier, and data sharing members in a data tier. This grouping enables a workload manager to make recommendations (to load balancer appliances) about how to distribute workload connections, e.g., based on metrics gathered from both the application and data tiers. In this approach, both applications and data sources are grouped into a workload grouping, and health, status and capacity information about both of these tiers (application and data) is then used to determine an overall distribution policy for the workload. These different tiers can reside on the same or different operating system environments.

Abstract: A periscope comprises an object window (4), a first reflector (5), a second reflector (6), a viewing window (7) and a display port for an electronic display (8). The first reflector (5) is arranged to reflect light from the object window (4) towards the second reflector (6). The second reflector (6) is selectively movable between a first position of use in which the second reflector (6) reflects light from the first reflector (5) towards the viewing window (7) and a second position of use in which the second reflector (6) reflects light from the display port towards the viewing window (7). The periscope provides a simple design that allows the user's view to be switched between an electronically generated image, such as night vision, and a purely optical image.

Abstract: The present invention relates generally to medical devices containing nanoporous surfaces and methods for making same. More specifically, the invention relates to implantable vascular stents or other biomedical devices having at least one dealloyed nanoporous layer that promotes improved cellular adhesion properties that promote healing and long term biocompatibility. In the case of stents, the nanoporous layer promotes re-endothelialization at sites of stent implantation vasculature, improves overall healing, and reduces inflammation and intimal disease progression. The nanoporous layer may be optionally loaded with one or more therapeutic agent to further improve the function of the implanted stent and further augment clinical efficacy.

Abstract: A method, apparatus and computer program product that allows for maintaining correct states of all sub-components in a state machine, even as sub-components leave the state machine and later rejoin in some previous state. Preferably, this is achieved without requiring the system to remember the states of all sub-components or a log of every event that was fed into the state machine. Thus, the technique does not require any knowledge of the previous state of the sub-components nor the need to preserve a complete log of events that were fed into the state machine. The state machine may be used to enhance the operation of a technological process, such as a workload management environment.

Abstract: A method, apparatus and computer program product that allows for maintaining correct states of all sub-components in a state machine, even as sub-components leave the state machine and later rejoin in some previous state. Preferably, this is achieved without requiring the system to remember the states of all sub-components or a log of every event that was fed into the state machine. Thus, the technique does not require any knowledge of the previous state of the sub-components nor the need to preserve a complete log of events that were fed into the state machine. The state machine may be used to enhance the operation of a technological process, such as a workload management environment.

Abstract: A method, apparatus and computer program product that allows for maintaining correct states of all sub-components in a state machine, even as sub-components leave the state machine and later rejoin in some previous state. Preferably, this is achieved without requiring the system to remember the states of all sub-components or a log of every event that was fed into the state machine. Thus, the technique does not require any knowledge of the previous state of the sub-components nor the need to preserve a complete log of events that were fed into the state machine. The state machine may be used to enhance the operation of a technological process, such as a workload management environment.