Abstract: Embodiments of systems, methods, and compositions provided herein relate to on bead tagmentation and droplet indexing. Some embodiments include performing co-assays on partitioned beads, including nucleic acid sequencing, indexed PCR, preparing nucleic acid libraries, determining methylation status, identifying genomic variants, or protein analysis.

Abstract: This application describes methods of preparing an immobilized library of tagged RNA fragments. Also described herein are a number of methods of preparing DNA and RNA sequencing libraries from a single sample. These methods can include library preparation from single cells.

Abstract: The present invention comprises systems and devices for sequencing of nucleic acid, such as short DNA sequences from clonally amplified single-molecule arrays.

Abstract: The present invention is directed to a method for isothermal amplification of a plurality of different target nucleic acids, wherein the different target nucleic acids are amplified using universal primers and colonies produced thereby can be distinguished from each other. The method, therefore, generates distinct colonies of amplified nucleic acid sequences that can be analyzed by various means to yield information particular to each distinct colony.

Abstract: The present invention relates to universal-tagged oligonucleotide primers, and to methods of using the primers for amplifying the genome.

Abstract: The present invention relates to universal-tagged oligonucleotide primers, and to methods of using the primers for amplifying the genome.

Abstract: The present invention relates to the detection of target sequences. Detection can be achieved through the use of ID-tag complexes. These ID-tag complexes are relatively stable in the absence of a target sequence. In the presence of a target sequence, the complexes dissociate and form new complexes or duplexes, which can be purified or eliminated and detected on an ID-tag system.

Abstract: The teachings relate to microarray controls.

Abstract: The present invention features “molecular torches” and the use of molecular torches for detecting the presence of a target nucleic acid sequence. Molecular torches contain a target binding domain, a target closing domain, and a joining region. The target binding domain is biased towards the target sequence such that the target binding domain forms a more stable hybrid with the target sequence than with the target closing domain under the same hybridization conditions. The joining region facilitates the formation or maintenance of a closed torch.

Abstract: The present invention features “molecular torches” and the use of molecular torches for detecting the presence of a target nucleic acid sequence. Molecular torches contain a target binding domain, a target closing domain, and a joining region. The target binding domain is biased towards the target sequence such that the target binding domain forms a more stable hybrid with the target sequence than with the target closing domain under the same hybridization conditions. The joining region facilitates the formation or maintenance of a closed torch.

Abstract: A chemiluminescent assay to determine the presence and/or amount of one or more labeled target molecules in a sample is described in which the surface layer of a solid support is contacted with a composition comprising first and second chemiluminescent substrates capable of being activated by first and second enzymes, respectively. A plurality of probes are disposed on the surface layer in discrete areas. At least some of the probes are bound to a first enzyme conjugate comprising the first enzyme and at least some of the probes are bound to a second enzyme conjugate comprising the second enzyme. The resulting chemiluminescent signals are then detected. The method can be used to compare two biological samples (e.g., mRNA populations from different cells) on the same support surface or to provide a chemiluminescent control signal for normalizing chemiluminescent assay data from a biological sample.

Abstract: Chemiluminescent assays to determine the presence and/or amount of one or more labeled analytes in a sample are described wherein a solid support is contacted sequentially with first and second chemiluminescent substrates each of which are capable of being activated by an enzyme and the resulting chemiluminescent signals are detected. A plurality of probes are disposed on a surface layer of the solid support in a plurality of discrete areas. Some of the probes are bound to a conjugate of the first enzyme and some of the probes are bound to a conjugate of the second enzyme. The assay can be used to compare biological samples (e.g., mRNA populations from different cells) on the same support surface. Alternatively, one of the chemiluminescent signals generated can be used as a control signal for normalizing the chemiluminescent assay data.

Abstract: The invention provides methods and kits for cDNA synthesis and for RNA amplification.

Abstract: The present invention relates to universal-tagged oligonucleotide primers, and to methods of using the primers for amplifying the genome.