Patents by Inventor Gary Withey

Gary Withey has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11098304
    Abstract: Provided herein are methods of identifying the origin of a nucleic acid sample. The methods include forming a reaction mixture comprising a nucleic acid sample comprising nucleic acid molecules from a single cell and a set of barcodes, incorporating the set of barcodes into the nucleic acid molecules of the sample, and identifying the set of barcodes incorporated into the nucleic acid molecules of the single cell thereby identifying the origin of the nucleic acid sample.
    Type: Grant
    Filed: November 4, 2016
    Date of Patent: August 24, 2021
    Assignee: ATRECA, INC.
    Inventor: Gary Withey
  • Patent number: 10316345
    Abstract: Provided herein are methods and compositions for analyzing nucleic acids associated with single cells using nucleic acid barcodes. According to some embodiments, a method for producing one or more polynucleotides of interest comprises: obtaining a plurality of RNAs associated with one or more samples, wherein the samples are obtained from one or more subjects, each RNA is associated with a single sample, and the RNAs associated with each sample are present in a separate reaction volume; adding an adapter molecule to the RNAs associated with each sample, wherein the adapter molecule is generated using an enzymatic reaction and comprises a universal priming sequence, a barcode sequence, and a binding site; and incorporating the barcode sequence into one or more polynucleotides associated with each sample, thereby producing the one or more polynucleotides of interest.
    Type: Grant
    Filed: February 8, 2017
    Date of Patent: June 11, 2019
    Assignee: ATRECA, INC.
    Inventors: Yann Chong Tan, Gary Withey
  • Publication number: 20170369921
    Abstract: Provided herein are methods and compositions for analyzing nucleic acids associated with single cells using nucleic acid barcodes. According to some embodiments, a method for producing one or more polynucleotides of interest comprises: obtaining a plurality of RNAs associated with one or more samples, wherein the samples are obtained from one or more subjects, each RNA is associated with a single sample, and the RNAs associated with each sample are present in a separate reaction volume; adding an adapter molecule to the RNAs associated with each sample, wherein the adapter molecule is generated using an enzymatic reaction and comprises a universal priming sequence, a barcode sequence, and a binding site; and incorporating the barcode sequence into one or more polynucleotides associated with each sample, thereby producing the one or more polynucleotides of interest.
    Type: Application
    Filed: February 8, 2017
    Publication date: December 28, 2017
    Inventors: Yann Chong Tan, Gary Withey
  • Patent number: 9580736
    Abstract: Provided herein are methods and compositions for analyzing nucleic acids associated with single cells using nucleic acid barcodes. According to some embodiments, a method for producing one or more polynucleotides of interest comprises: obtaining a plurality of RNAs associated with one or more samples, wherein the samples are obtained from one or more subjects, each RNA is associated with a single sample, and the RNAs associated with each sample are present in a separate reaction volume; adding an adapter molecule to the RNAs associated with each sample, wherein the adapter molecule is generated using an enzymatic reaction and comprises a universal priming sequence, a barcode sequence, and a binding site; and incorporating the barcode sequence into one or more polynucleotides associated with each sample, thereby producing the one or more polynucleotides of interest.
    Type: Grant
    Filed: December 30, 2014
    Date of Patent: February 28, 2017
    Assignee: Atreca, Inc.
    Inventors: Yann Chong Tan, Gary Withey
  • Publication number: 20160264924
    Abstract: Methods and systems are provided for merging a droplet with a volume of fluid in a microfluidic system. In particular, the methods of the invention use a microfluidic structure designed to merge a fluid with a droplet in order to dilute, add volume, or add selected reagents, biological materials, or synthetic materials to a droplet. Also provided are related systems and methods for cell lysis.
    Type: Application
    Filed: May 20, 2016
    Publication date: September 15, 2016
    Inventors: David Scott Johnson, Gary Withey, Philip Homewood, Timothy Atkins
  • Publication number: 20150154352
    Abstract: Methods and systems are provided for massively parallel genetic analysis of single cells in emulsions or droplets. A biological sample is divided into subsamples of single cells or cell supbopulations, and a fusion complex is formed by molecular linkage and amplification techniques. Methods, apparatuses, and systems are provided for high-throughput, massively parallel analysis of the subsamples. These methods integrate molecular, algorithmic, and engineering approaches. They have broad and useful application in a number of biological and medical fields, including immunology, noninvasive prenatal diagnosis, and noninvasive cancer diagnosis.
    Type: Application
    Filed: June 21, 2013
    Publication date: June 4, 2015
    Applicant: GigaGen, Inc.
    Inventors: David Scott Johnson, Andrea Loehr, Thomas Hunt, Everett Hurteau Meyer, Walter Mathias Howell, Gary Withey
  • Publication number: 20150125865
    Abstract: Methods and systems are provided for merging a droplet with a volume of fluid in a microfluidic system. In particular, the methods of the invention use a microfluidic structure designed to merge a fluid with a droplet in order to dilute, add volume, or add selected reagents, biological materials, or synthetic materials to a droplet. Also provided are related systems and methods for cell lysis.
    Type: Application
    Filed: December 20, 2012
    Publication date: May 7, 2015
    Inventors: David Scott Johnson, Gary Withey, Philip Homewood, Timothy Atkins