Abstract: The invention relates to the use of mutants of L. casei having at least a mutation impairing the regulation of a carbon catabolite repression (CCR) mechanism involving the PTS protein HPr, for the preparation of a food product. The use of said mutants allows for instance to impart to said food products an improved texture and flavor, and/or a higher content in aroma compounds.

Abstract: The invention relates to the use of mutants of L. casei having at least a mutation impairing the regulation of a carbon catabolite repression (CCR) mechanism involving the PTS protein HPr, for the preparation of a food product. The use of said mutants allows for instance to impart to said food products an improved texture and flavor, and/or a higher content in aroma compounds.

Abstract: The invention relates to products which are fermented with probiotic lactic bacteria from milk or a shake comprising almonds (Prunus Amygdalus, var. dulcis), other tree nuts or orgeat obtained from chufa tubers (Cyperus esculentus). The inventive products deliver the nutritional benefits of the tree nuts, the chufa orgeat and the added probiotic bacteria, with a unique flavour and a consistency, acidity and aroma similar to yoghurt. Optionally, if the sugar content is kept low, the products have astringent and intestinal flora re-establishment properties. In addition, the products can be combined with additives and flavours and enriched with minerals, vitamins, fibre or oligosaccharides having a bifidogenic effect, etc., or pasteurised following fermentation. In almost all cases, the products constitute an excellent nutritional supplement for adolescents, athletes or elderly persons.

Abstract: This invention describes the use of a method for the expression of proteins in bacteria, which is based on the inducer effect, for example, of a carbohydrate on the anti-terminating activity of a protein of the family of anti-terminators, B1gG/SacY. The procedure has been tested with the system derived from the operon lacTEGF, inducible by lactose in Lactobacillus casei, for the expression of proteins of different kinds. In the invention are included examples of expression in multicopy plasmids or of integration in the chromosomal operon lacTEGF, thus achieving the coordinated expression of the desired genes together with those that code the enzymes of the metabolism of the lactose.