Abstract: A lattice and distribution network for a stackable chromatography cassette comprising: a peripheral seal; at least one screen forming the lattice surrounded by the peripheral seal, each at least one screen comprising a plurality of struts in a latticed arrangement; a first internal distribution network fluidly coupled to the lattice and surrounded by the peripheral seal; a second internal distribution network disposed opposite the first internal distribution network, fluidly coupled to the lattice and surrounded by the peripheral seal; wherein a direction of fluid flow is established from the first internal distribution network through the lattice to the second internal distribution network; and wherein preferential streamlines are minimized.

Abstract: A system, method and device are disclosed for bio-processing a feed stream and providing a constant output by operating a continuous single-pass tangential-flow process. The single-pass process provides high conversion concentration while operating at relatively low feed flow rates, and the process can also be used to provide constant output diafiltration.

Abstract: A cycling ultra-thin channel filtration (cUTF) method, system and module are disclosed for concentration and purification of biomolecules. In one embodiment, a cUTF system includes a cUTF module and a cycle controller to load a feed aliquot of a feed stream into the cUTF module inducing permeation of the feed stream to build a boundary layer by pressurizing the feed stream, to stop permeation and to recover a retentate aliquot in a repeating cycle.

Abstract: Adsorptive bed devices include a scaffold in housing having a stress absorbing rigid structure and open cells filled with adsorptive beads. The scaffold restricts movement of the plurality of adsorptive beads, absorbs stress induced by a hydraulic pressure gradient along a direction of liquid flow and transfers a portion of the induced compressive stress to the housing. In one embodiment the adsorptive bed is packed into a chromatography column, and in another embodiment the adsorptive bed is sealed in a monolithic block. In another embodiment, the adsorptive bed device includes an adsorptive block, first and second planar distributors and peripheral seal. The adsorptive media includes multiple layers of planarly cohesive media and when operated in a shallow bed configuration possesses significant tensile strength along its planar dimensions enabling it to support the hydraulic pressures that will be generated by the fluids being processed through the adsorptive devices.

Abstract: A cycling ultra-thin channel filtration (cUTF) method, system and module are disclosed for concentration and purification of biomolecules. In one embodiment, a cUTF system includes a cUTF module and a cycle controller to load a feed aliquot of a feed stream into the cUTF module inducing permeation of the feed stream to build a boundary layer by pressurizing the feed stream, to stop permeation and to recover a retentate aliquot in a repeating cycle.

Abstract: A cycling ultra-thin channel filtration (cUTF) method, system and module are disclosed for concentration and purification of biomolecules. In one embodiment, a cUTF system includes a cUTF module and a cycle controller to load a feed aliquot of a feed stream into the cUTF module inducing permeation of the feed stream to build a boundary layer by pressurizing the feed stream, to stop permeation and to recover a retentate aliquot in a repeating cycle.

Abstract: Adsorptive bed devices include a scaffold in housing having a stress absorbing rigid structure and open cells filled with adsorptive beads. The scaffold restricts movement of the plurality of adsorptive beads, absorbs stress induced by a hydraulic pressure gradient along a direction of liquid flow and transfers a portion of the induced compressive stress to the housing. In one embodiment the adsorptive bed is packed into a chromatography column, and in another embodiment the adsorptive bed is sealed in a monolithic block. In another embodiment, the adsorptive bed device includes an adsorptive block, first and second planar distributors and peripheral seal. The adsorptive media includes multiple layers of planarly cohesive media and when operated in a shallow bed configuration possesses significant tensile strength along its planar dimensions enabling it to support the hydraulic pressures that will be generated by the fluids being processed through the adsorptive devices.

Abstract: A method and apparatus for operating an electro-deionization (EDI) module produces purified water using a power supply controller to provide more efficient and accurate operation.

Abstract: Adsorptive bed devices include a scaffold in housing having a stress absorbing rigid structure and open cells filled with adsorptive beads. The scaffold restricts movement of the plurality of adsorptive beads, absorbs compressive stress induced by a hydraulic pressure gradient along a direction of liquid flow and transfers a portion of the induced compressive stress to the housing. In one embodiment the adsorptive bed in packed into a chromatography column, and in another embodiment the adsorptive bed is sealed in a monolithic block.

Abstract: Adsorptive bed devices include a scaffold in housing having a stress absorbing rigid structure and open cells filled with adsorptive beads. The scaffold restricts movement of the plurality of adsorptive beads, absorbs compressive stress induced by a hydraulic pressure gradient along a direction of liquid flow and transfers a portion of the induced compressive stress to the housing. In one embodiment the adsorptive bed in packed into a chromatography column, and in another embodiment the adsorptive bed is sealed in a monolithic block.

Abstract: A system, method and device are disclosed for bio-processing a feed stream and providing a constant output by operating a continuous single-pass tangential-flow process. The single-pass process provides high conversion concentration while operating at relatively low feed flow rates, and the process can also be used to provide constant output diafiltration.

Abstract: A separation module and method are disclosed for processing a liquid sample and providing high conversion by operating a single-pass tangential-flow process without a recirculation loop. In one embodiment, the separation module includes three reservoirs and has at least one long, thin channel with a large ratio of channel membrane area to: channel void volume; volume of a sample feed reservoir; and volume of the feed sample. In another embodiment, the separation module includes an external feed reservoir, an external retentate reservoir fluidly and a first vacuum source adapted to apply a first vacuum to the separation element disposed within the housing. The single-pass TFF separation element and single-pass process provides high conversion while operating with relatively low pressure sources. Certain embodiments disclosed herein can be used for the separation of blood.

Abstract: A system, method and device are disclosed for bio-processing a feed stream and providing a constant output by operating a continuous single-pass tangential-flow process. The single-pass process provides high conversion concentration while operating at relatively low feed flow rates, and the process can also be used to provide constant output diafiltration.

Abstract: Stackable planar adsorption devices include a plurality of layers of adsorptive media provided in a web format. The layers are stacked in contiguous fashion, sealed and include fluid passageways to provide a range of scalable chromatography devices suitable for large scale manufacturing applications.

Abstract: A functional, porous, interpenetrating polymer network (IPN) includes a first polymer network in the form of a porogenic support fabric (PSF) composed of linear polymers in the form of a pre-formed network comprising a fibrous composite and a second polymer network synthesized, gelated, and/or cross-linked in the presence of the first polymer network to form a system of polymers which have their respective chains held in place by means of permanent physical entanglements produced by the interweaving of the component polymer networks. The IPN is modified by dissolving and dispersing a portion of the PSF fibers, the dispersible fiber network (DFN) to form a pre-designed interconnected pore structure. The resultant porous, supported, second polymer network has convective flow, diffusive flow, and high capacity, and may include functional capture chemistries to provide an adsorptive media for chromatography and filtration of various compounds including biomolecules.

Abstract: A separation module and method are disclosed for processing a liquid sample and providing high conversion by operating a single-pass tangential-flow process without a recirculation loop. In one embodiment, the separation module includes three reservoirs and has at least one long, thin channel with a large ratio of channel membrane area to: channel void volume; volume of a sample feed reservoir; and volume of the feed sample. In another embodiment, the separation module includes two reservoirs and a hydrophobic vent. The single-pass process provides high conversion while operating with relatively low pressure sources.

Abstract: A system, method and device are disclosed for bio-processing a feed stream providing a constant output by operating a continuous single-pass tangential-flow process. Embodiments are operated without large circulation pumps. Other embodiments use components which can be disposed after a single use. The system, method and device can also be used to provide constant output diafiltration.

Abstract: A system, method and device are disclosed for bio-processing a feed stream and providing a constant output by operating a continuous single-pass tangential-flow process. The single-pass process provides high conversion concentration while operating at relatively low feed flow rates, and the process can also be used to provide constant output diafiltration.

Abstract: A system, method and device are disclosed for bio-processing a feed stream and providing a constant output by operating a continuous single-pass tangential-flow process. The single-pass process provides high conversion concentration while operating at relatively low feed flow rates, and the process can also be used to provide constant output diafiltration.

Abstract: A functional, porous, interpenetrating polymer network (IPN) includes a first polymer network in the form of a porogenic support fabric (PSF) composed of linear polymers in the form of a pre-formed network comprising a fibrous composite and a second polymer network synthesized, gelated, and/or cross-linked in the presence of the first polymer network to form a system of polymers which have their respective chains held in place by means of permanent physical entanglements produced by the interweaving of the component polymer networks. The IPN is modified by dissolving and dispersing a portion of the PSF fibers, the dispersible fiber network (DFN) to form a pre-designed interconnected pore structure. The resultant porous, supported, second polymer network has convective flow, diffusive flow, and high capacity, and may include functional capture chemistries to provide an adsorptive media for chromatography and filtration of various compounds including biomolecules.