Abstract: The present disclosure provides mammalian cell lines for expression of viral vectors, and methods of making and using the same. Provided methods employ use of identifiers that are capable of being packaged into a viral vector to select and/or identify mammalian cell lines with engineered sequences associated with beneficial characteristics for viral vectors production. Exemplary viral vectors include AAV vectors.

Abstract: The present disclosure provides mammalian cell lines for expression of viral vectors, and methods of making and using the same. Provided methods employ use of identifiers that are capable of being packaged into a viral vector to select and/or identify mammalian cell lines with engineered sequences associated with beneficial characteristics for viral vectors production. Exemplary viral vectors include AAV vectors.

Abstract: A method for removal of sulfide from a stream, the method comprising preparing a metal-chelant complex, designated M-Yn, where M is a divalent or trivalent cation, Y comprises a gluconic acid, glucaric acid or derivative thereof, and n is from about 1 to about 3; contacting a mixture comprising M-Yn and a base with a sulfide stream under conditions suitable for the formation of a metal-sulfide species; and contacting the metal-sulfide species with an oxidizing agent to form a soluble metal sulfide species. A method for removal of sulfide from a stream, the method comprising preparing a sulfide scavenging mixture comprising a base and a metal-complex characterized by Structure (I); contacting the sulfide scavenging mixture with a sulfide stream under conditions suitable for the formation of a metal-sulfide species; and contacting the metal-sulfide species with an oxidizing agent to form a soluble metal sulfide species.

Abstract: A method for removal of sulfide from a stream, the method comprising preparing a metal-chelant complex, designated M-Yn, where M is a divalent or trivalent cation, Y comprises a gluconic acid, glucaric acid or derivative thereof, and n is from about 1 to about 3; contacting a mixture comprising M-Yn and a base with a sulfide stream under conditions suitable for the formation of a metal-sulfide species; and contacting the metal-sulfide species with an oxidizing agent to form a soluble metal sulfide species. A method for removal of sulfide from a stream, the method comprising preparing a sulfide scavenging mixture comprising a base and a metal-complex characterized by Structure I; contacting the sulfide scavenging mixture with a sulfide stream under conditions suitable for the formation of a metal-sulfide species; and contacting the metal-sulfide species with an oxidizing agent to form a soluble metal sulfide species.

Abstract: A composition for efficient metal chelation includes (i) a primary chelant; (ii) a coordination enhancing additive; and (iii) a solvent. A wellbore servicing fluid includes (i) a primary chelant; (ii) a coordination enhancing additive; and (iii) a solvent.

Abstract: A chemoenzymatic process for the preparation of an oxidized glucose product comprising contacting D-glucose with an enzyme selected from the group consisting essentially of galactose oxidase (GAO), glucose oxidase (GOX), polysaccharide monooxygenase, catalase, animal peroxidase, periplasmic aldehyde oxidase (Pao), unspecific peroxygenase (UPO), lactoperoxidase (LPO), myeloperoxidase (MPO), eosinophil peroxidase (EPO), thyroid peroxidase (TPO), ovoperoxidase, salivary peroxidase, vanadium haloperoxidase, non-mammalian vertebrate peroxidase (POX), peroxidasin (Pxd), bacterial peroxicin (Pxc), invertebrate peroxinectin (Pxt), short peroxidockerin (PxDo), alpha-dioxygenase (aDox), dual oxidase (DuOx), prostaglandin H synthase (PGHS), cyclooxygenase (CyOx), linoleate diol synthase (LDS), variants thereof, and combinations thereof under conditions suitable for the formation of an oxidized intermediate; and contacting the oxidized intermediate with a metal catalyst to form an oxidized glucose product.

Abstract: A system for the production of high value chemicals includes (a) an input selected from the group consisting of ethylene glycol, glycerol, ethanol methanol or a combination thereof. In addition, the system includes (b) an oxidation biocatalyst including an alcohol oxidase, a copper radical oxidase, a glycerol oxidase, an alditol oxidase or a combination thereof. Further, the system includes (c) an oxidized intermediate. The system also includes (d) a finishing catalyst including a supported metal catalyst, a carboligating catalyst, an amine oxidase, a glyoxalase, an acid catalyst, a base catalyst, an isomerization catalyst or a combination thereof. Still further, the system includes (e) an output.

Abstract: A chemoenzymatic process for the preparation of 2,5-furan dicarboxylic acid includes contacting D-glucose with (i) at least two enzymes selected from the group consisting essentially of galactose oxidase, pyranose 2-oxidase, glucarate dehydratase, catalase and a combination thereof to produce an intermediate; and (ii) a heterogeneous metal catalyst to form 2,5-furan dicarboxylic acid.

Abstract: A method of purifying a produced water comprising contacting a produced water stream with a composition comprising a (i) a chelant; (ii) an oxidizing agent; and (iii) a surfactant under conditions suitable for the formation of a purified produced water. A composition for purifying produced water comprising (i) a biochelant in an amount of from about 1 wt. % to about 10 wt. %: (ii) an oxidizing agent in an amount of from about 3 wt. % to about 50 wt. %; (iii) a surfactant in an amount of from about 0.1 wt. % to about 70 wt. % wherein the weight percentage is based on the total weight of the composition; and (iv) a solvent.

Abstract: A hydrogen peroxide and gluconic acid production method and system is disclosed that can include receiving an aqueous solution having glucose, water, and glucose oxidase at a reaction chamber. Here, the reaction chamber facilitates an enzymatic reaction between a gas phase and a liquid phase of the aqueous solution, thereby yielding a first solution comprising hydrogen peroxide, gluconic acid, and the glucose oxidase. The method can further include receiving the first solution at a separation chamber, wherein the separation chamber is comprised of a semi-permeable membrane having a pre-defined molecular weight barrier for separating the glucose oxidase, thereby resulting in a combined hydrogen peroxide and gluconic acid solution. The method can further include at least partially converting the gluconic acid into a gluconate salt, and separating and concentrating the hydrogen peroxide from the gluconic acid or gluconate salt via vacuum flash evaporation and vacuum distillation.

Abstract: A hydrogen peroxide production method, system, and apparatus is provided for producing large volumes of hydrogen peroxide having concentrations up to and excess of 80% in one continuous cycle. In one aspect, the hydrogen peroxide production system can include an aqueous solution comprised of an NQO1 enzyme, an NQO1 activated compound or molecule, and an NADH or NADPH cofactor for producing hydrogen peroxide, a production chamber having a semi-permeable membrane for receiving the aqueous solution. Here, the membrane can further include one or more molecular weight barriers configured to diffuse the produced hydrogen peroxide there through. The system can also include a collection chamber for receiving the produced hydrogen peroxide, and one or more pumps for circulating the aqueous solution having the hydrogen peroxide to the collection chamber and back to the production chamber.

Abstract: A hydrogen peroxide production method, system, and apparatus is provided for producing large volumes of hydrogen peroxide having concentrations up to and excess of 80% in one continuous cycle. In one aspect, the method can include mixing NQO1 enzyme, an NQO1 activated compound or molecule, and an NADH or NADPH cofactor with an aqueous solution, dispensing the aqueous solution within or onto a semi-permeable membrane, wherein the semi-permeable membrane further includes a pre-defined molecular weight barrier. In addition, an oxidation-reduction reaction of the NQO1 enzyme, the NQO1 activated compound or molecule, and the NADH or NADPH cofactor within the aqueous solution produce hydrogen peroxide at a concentration level. Here, the produced hydrogen peroxide is above the pre-defined molecular weight barrier of the semi-permeable membrane and diffuses through the semi-permeable membrane to be extracted for use.