Abstract: Disclosed herein are details of a hermetically or aseptically sealed bioreactor. The bioreactor comprises a bioreactor chamber, a membrane wall, a scaffold structure, a linear actuator, a linear transfer means, and a control system. Use of the bioreactor permits the inner scaffold structure to be moved and manipulated while still preserving a hermetic or aseptic seal inside the bioreactor chamber during operation.

Abstract: The present invention provides a bioreactor controller with a simple user interface comprising a microcontroller and a linear motor. The bioreactor controller is microcontroller-based, has a greater temporal accuracy, and inexpensive. The microcontroller of the bioreactor controller selects a protocol mode and a setup mode by the user, runs the protocol mode if the setup mode is not selected by the user, stops running the protocol mode for a predetermined time if the protocol mode is selected by the user until the user is completed with calculations and record-keeping, detects movement error of the bioreactor controller, and informs the error to the user.

Abstract: The present invention provides a bioreactor controller with a simple user interface comprising a microcontroller and a linear motor. The bioreactor controller is microcontroller-based, has a greater temporal accuracy, and inexpensive. The microcontroller of the bioreactor controller selects a protocol mode and a setup mode by the user, runs the protocol mode if the setup mode is not selected by the user, stops running the protocol mode for a predetermined time if the protocol mode is selected by the user until the user is completed with calculations and record-keeping, detects movement error of the bioreactor controller, and informs the error to the user.

Abstract: This disclosure provides for apparatuses, systems and methods for in vitro sample detection. For example in one embodiment, this disclosure provides an automated Pe-toner microfluidic device (and related method) on a centrifugal platform for DNA sample lysis and DNA extraction. A second embodiment provides a system and method for qualitative detection, quantification, and real-time monitoring of nucleic acid amplification products using magnetic bead aggregation inhibition. A third embodiment provides a platform for simultaneous detection of mRNA markers from blood, cell-free semen, sperm, saliva, and vaginal fluid. The third embodiment comprises a system and method that provide for simple, rapid, and fluorescence-free detection of body fluids using mRNA marker amplification and optical detection for mRNA marker analysis with a smart phone with image analysis.