Abstract: The present disclosure provides a method of suppressing oomycete infection, such as a Phytophthora infection, or triggering programmed cell death in Solanaceae plants comprising administering at least one modulator, wherein the at least one modulator modulates at least one of the genes listed in Table 1 or variants or homologs thereof. The present disclosure also provides compositions useful in suppressing oomycete infection, such as Phytophthora infection and triggering programmed cell death and screening assays for identifying substances useful in suppressing oomycete infection, such as Phytophthora infection and triggering programmed cell death.

Abstract: After-cooking darkening is a gray-black discoloration of the potato tuber, formed after cooking by the oxidation of an iron-chlorogenic acid complex. Cinnamic acid 4-hydroxylase (C4H) is a key enzyme involved in the biosynthesis of chlorogenic acid. The full-length c4h gene was cloned and sequenced from both genomic DNA and cDNA of Russet Burbank tuber tissue by PCR and 5? and 3? RACE. The gene expression levels of c4h were examined by Northern hybridization, relative quantitative RT-PCR and real time quantitative RT-PCR in potato cultivars and wide selection of diploid clones varying in susceptibility to after-cooking darkening. Results suggest that there is a relationship between the levels of c4h gene expression and the degree of after-cooking darkening in potato tubers. The inhibition of C4H gene expression and over expression of C4H expression were also examined.

Abstract: Proteins that are associated with increased after-cooking darkening (ACD) are described. The proteins are useful in diagnostic assays for detecting ACD. Inhibiting or activating the proteins can also be useful in controlling and/or reducing ACD.

Abstract: After-cooking darkening is a gray-black discoloration of the potato tuber, formed after cooking by the oxidation of an iron-chlorogenic acid complex. Cinnamic acid 4-hydroxylase (C4H) is a key enzyme involved in the biosynthesis of chlorogenic acid. The full-length c4h gene was cloned and sequenced from both genomic DNA and cDNA of Russet Burbank tuber tissue by PCR and 5? and 3? RACE. The gene expression levels of c4h were examined by Northern hybridization, relative quantitative RT-PCR and real time quantitative RT-PCR in potato cultivars and wide selection of diploid clones varying in susceptibility to after-cooking darkening. Results suggest that there is a relationship between the levels of c4h gene expression and the degree of after-cooking darkening in potato tubers. The inhibition of C4H gene expression and over expression of C4H expression were also examined.