Abstract: An interference ribonucleic acid containing specific sequence for inhibiting MMP1, which is composed of 19 to 25 nucleotides, wherein the interference ribonucleic acid has a sequence selected from the group consisted of the sequences as set forth in, or essentially identical to, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, and SEQ ID NO: 10. By means of the interference ribonucleic acid and MMP1 with high specificity binding, gene and protein expression of endogenous MMP1 of cancer cell can be inhibited, and then the interference ribonucleic acid can be used for pharmaceutical composition to prevent or treat cancer metastasis or to improve wrinkle resistance.

Abstract: An E. coli expression system for producing mature human tyrosinase is provided and includes an E. coli host, which has a trait for expressing endogenous methionyl aminopeptidase in cytoplasm, and an expression vector transformed into the E. coli host. The expression vector has a replication origin sequence of an E. coli, and the expression vector includes an inducible promoter and a DNA fragment of human tyrosinase having a sequence referenced as SEQ ID NO:3, wherein a 5? end of the DNA fragment is constructed at a restriction enzyme NdeI recognition site located at the downstream of the inducible promoter.