Abstract: A primer set and a kit is used for simultaneous identification of a classical strain and a variant strain of a porcine epidemic diarrhea virus (PEDV). The primer set includes primer set I and primer set II; where primer set I comprises the nucleotide sequences set forth in SEQ ID NO: 1 to SEQ ID NO: 4; and primer set II comprises the nucleotide sequences set forth in SEQ ID NO: 5 to SEQ ID NO: 8. This approach not only helps avoid false negatives or false positives in the detection of PEDV variant strain infections, but also enables the identification between classical strain and variant strain in PEDV. This is of significant importance for understanding the prevalence of PEDV and for the prevention and control of PEDV infections.