Abstract: CGTases, which, when converting starch or starch-like substrates to CD, produce &ggr;-CD to an increased extent and still exhibit at least 60% of the specific total CGTase activity of the starting CGTase which was used for preparing the enzyme concerned. The amino acid sequences differ from the amino acid sequences of known CGTases by the deletion of from 3 to 8 amino acids from the region from amino acid position 155 up to and including amino acid position 195, where position 1 of the protein sequence is the beginning of the signal peptide of the CGTase and the deletion brings about the increase in the &ggr;-CGTase activity of the protein.

Abstract: A cyclodextrin glycosyltransferase (CGTase) which, in the conversion of starch or starch-like substrates to CD, produces .gamma.-CD in an increased amount, and whose protein sequence, in the region between amino acid position 180 and amino acid position 240, contains the amino acid sequence (SEQ ID NO: 1), where position 1 of the protein sequence is the beginning of the signal peptide of the CGTase and Xxx denotes a natural amino acid.