Abstract: Disclosed are methods and systems for analyzing exhaled breath aerosol particles present in the ambient environment using high flowrate aerosol collector systems and sensitive and specific nucleic acid analysis systems to determine if active spreaders of a respiratory disease are present in an indoor space. The disclosed systems and methods provide for a diagnostic test result in less than about 30 minutes.

Abstract: A novel inexpensive post-dip formulation for the use of chlorine-based post-dip disinfectant. The present invention provides a new pre-dip or post-dip formulation with the following properties: Inexpensive; Easy to produce and mix at the site of application from readily available components; Components which can be delivered in dry form for reduced bulk; Harmless, non-irritating for the teat skin and for the skin of the milkers; Highly effective bactericide in pre and post-dip solutions for mastitis prevention; Yields only environmentally friendly byproducts.

Abstract: A method for increasing the production of desired hemoproteins is described in which the rate-limiting step in a heme biosynthetic pathway is deregulated to increase the quantity of the rate-limiting enzyme. In yeast, transforming cells with the HEM2 gene increases the quantity of the rate-limiting enzyme ALA dehydratase. Free heme concentrations are reduced in cells by providing heme binding agents that complexes with overproduced heme, thereby reducing or eliminating heme feedback inhibition in the cell. The present invention permits investigation of other factors effecting the heme biosynthetic pathway by reducing interference from heme feedback inhibition within that pathway.