Abstract: A sperm sorting device suitable for selectively isolating motile sperms from a semen specimen includes a base and a sorting unit. The base comprises a central protruding block including a truncated cone-shaped surface, a bottom wall that surrounds the central protruding block, and an intermediate ring. The intermediate ring connects to the bottom wall via an inner wall surface. The sorting unit includes a sorting ring and a protruding ring. The sorting ring includes a central hole for a sorting filter that filters the semen specimen and is beveled at an angle that increases a permeable surface of the sorting filter. The protruding ring connects to the sorting ring via an inner sleeve surface. The sperm sorting device enhances sperm sorting quality by allowing the motile sperms to swim upward through the first sorting filter naturally.