Abstract: A system monitors gas flow and pressure to a gas appliance in a fluid network comprising an analyzer. The analyzer has a housing defining an inlet, an outlet, and an interior in fluid communication with the inlet and the outlet. At least one sensor is coupled to the analyzer and configured to generate at least one signal related to gas being supplied to the gas appliance. A smart device communicates with the analyzer, wherein the smart device has a user interface and is configured to monitor, store and display data. The smart device can present any or all of a plurality of parameters such as the flow of gas, a capacity of the flow of gas, a temperature, a pressure of the gas and the like to a user based on signals from sensors.

Abstract: The present disclosure is generally directed to an agricultural formulation comprising an anucleated minicells and an anucleated cell-based platforms for encapsulation and scalable delivery of biologically active compounds. Disclosed herein are compositions for the stable and targeted delivery of biologically active compounds within achromosomal and/or anucleated cells onto and/or into a target cell. The present disclosure also provides methods of improving encapsulation and retention of biologically active compounds in achromosomal and/or anucleated cells and delivering biologically active compounds into a target cell.

Abstract: This disclosure to the methods for nucleic acid modification, gene targeting, and gene tagging comprising an engineered Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated transposon (CAST) system with a donor DNA comprising at least one engineered transposon end sequence and/or at least one integration co-factor protein. More particularly, the present disclosure provides systems comprising: an engineered CAST system or one or more nucleic acids encoding the engineered CAST system, wherein the CAST system comprises at least one or all of: i) at least one Cas protein, ii) one or more transposon-associated proteins, iii) at least one guide RNA (gRNA) complementary to at least a portion of a target nucleic acid sequence; and a donor nucleic acid comprising a cargo nucleic acid sequence flanked by at least one engineered transposon end sequence and/or at least one integration co-factor protein, or a nucleic acid encoding thereof.

Abstract: The present disclosure is generally directed to an agricultural formulation comprising an anucleated minicells and an anucleated cell-based platforms for encapsulation and scalable delivery of biologically active compounds. Disclosed herein are compositions for the stable and targeted delivery of biologically active compounds within achromosomal and/or anucleated cells onto and/or into a target cell. The present disclosure also provides methods of improving encapsulation and retention of biologically active compounds in achromosomal and/or anucleated cells and delivering biologically active compounds into a target cell.

Abstract: A dual flex footrest having separate elongated upper portions connected to elongated bottom portions via flexing portions, allowing a user to flex each foot individually to increase circulation to the user's body. The footrest aids in reducing leg swelling, leg cramps, restless leg syndrome, complications from arthritis and diabetes, neuropath, nerve pain and knee and foot pain. The footrest also aids in increasing oxygenated blood rich in nutrients, alertness, creativity, and productivity.

Abstract: The present disclosure provides systems, kits, and methods for nucleic acid integration utilizing engineered Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated transposon (CRISPR-Tn) system. More particularly, the present disclosure provides systems comprising: an engineered CRISPR-Tn system or one or more nucleic acids encoding the engineered CRISPR-Tn system, wherein the CRISPR-Tn system comprises at least one or both of: a) at least one Cas protein (e.g., Cas6, Cas7, Cas5, and/or Cas8); and b) one or more transposon-associated proteins (e.g., TnsA, TnsB, TnsC, TnsD, and/or TniQ). The present disclosure also provides systems, kits, and methods for nucleic acid integration in a eukaryotic cell.

Abstract: The present disclosure provides systems, kits, and methods provide systems and methods for recruiting one or more effector domains to a target nucleic acid and or modulating expression of a target gene in a cell utilizing an engineered Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR associated (Cas) system. More particularly, the present disclosure provides systems comprising: an engineered CRISPR-Cas system or one or more nucleic acids encoding the engineered CRISPR-Cas system, wherein the CRISPR-Cas system comprises: at least one Cas protein (e.g., Cas6, Cas7, Cas5, Cas8 and/or Cas12k); a guide RNA (gRNA) complementary to at least a portion of the target nucleic acid sequence; and, optionally, at least one transposon-associated protein (e.g., TniQ, TnsC, TnsA, and/or TnsB).

Abstract: A method for erecting a box includes positioning can unerected box in an automated erecting apparatus. The method further includes bending major and minor flaps on the unerected box relative to sides of the unerected box. The method further includes, while the major and minor flaps are bent relative to the sides of the unerected box, performing an automated opening operation on the unerected box, wherein the unerected box comprises a stacking fold.

Abstract: The present disclosure is generally directed to an anucleated cell-based platforms for encapsulation and delivery of agricultural compounds. Disclosed herein are compositions for the stable and targeted delivery of agricultural compounds within achromosomal and/or anucleated cells. The present disclosure also provides methods of improving encapsulation and retention of agricultural compounds in achromosomal and/or anucleated cells.

Abstract: The present disclosure is generally directed to an agricultural formulation comprising an anucleated minicells and an anucleated cell-based platforms for encapsulation and scalable delivery of biologically active compounds. Disclosed herein are compositions for the stable and targeted delivery of biologically active compounds within achromosomal and/or anucleated cells onto and/or into a target cell. The present disclosure also provides methods of improving encapsulation and retention of biologically active compounds in achromosomal and/or anucleated cells and delivering biologically active compounds into a target cell.

Abstract: A platen assembly for printing on face masks which includes a base member having a plurality of jig members mounted thereon. The base member has a top surface and a bottom surface, with its bottom surface operative to attach to a platen holding mechanism of a conventional direct to garment printer. Each of the jig members may be attached to the top surface of the base member and hold a single face mask in such a manner that it can be printed on. Edges of the base member and of each jig member include interface portions which allow portions of the face mask to be selectively threaded through to more effectively keep the face masks in place, being held smooth and taught.

Abstract: According to this disclosure, a method of synthesizing an audio stream sample using a processor is provided. The method comprises: generating a set of unnormalized log probabilities using a neural network, each unnormalized log probability associated with a possible value for the audio stream sample, sampling a Gumbel distribution for each of the unnormalized log probabilities, adding the samples from the Gumbel distribution to each of the respective unnormalized log probabilities to generate a set of modified log probabilities, each modified log probability associated with a possible value for the audio stream sample, and selecting the possible value of the audio stream sample associated with the largest modified log probability from the set of modified log probabilities as the audio stream sample.

Abstract: The present disclosure is generally directed to an agricultural formulation comprising an anucleated minicells and an anucleated cell-based platforms for encapsulation and scalable delivery of biologically active compounds. Disclosed herein are compositions for the stable and targeted delivery of biologically active compounds within achromosomal and/or anucleated cells onto and/or into a target cell. The present disclosure also provides methods of improving encapsulation and retention of biologically active compounds in achromosomal and/or anucleated cells and delivering biologically active compounds into a target cell.

Abstract: A box erecting machine includes a transport mechanism that can move an un-erected box from an entry portion of the box erecting machine to a forward portion of the box erecting machine. The transport mechanism can include pivot arm(s) and clamp(s) for securing and moving the un-erected box. The machine can also include a box erecting assembly that erects the un-erected box. The box erecting assembly can include an opening or unfolding mechanism that opens or unfolds the un-erected box into a generally rectangular tube. The machine can also include a folding mechanism and a closure mechanism for folding and securing the bottom flaps of the box.

Abstract: The present disclosure relates to compositions of immobilized enzymes on the surface of achromosomal and/or anucleate cells and uses thereof. In particular, the present disclosure provides genetically engineered minicells with enzymes self-assembled on their surface. The immobilized enzymes on the surface of achromosomal and/or anucleate minicells, has agricultural, industrial, and environmental applications due to their improved stability durability and, reusability. Also, provided are methods for producing and purifying enzyme-immobilized minicells.

Abstract: The present disclosure is generally directed to an anucleated cell-based platforms for encapsulation and delivery of agricultural compounds. Disclosed herein are compositions for the stable and targeted delivery of agricultural compounds within achromosomal and/or anucleated cells. The present disclosure also provides methods of improving encapsulation and retention of agricultural compounds in achromosomal and/or anucleated cells.

Abstract: The present disclosure relates to compositions of immobilized enzymes on the surface of achromosomal and/or anucleate cells and uses thereof. In particular, the present disclosure provides genetically engineered minicells with enzymes self-assembled on their surface. The immobilized enzymes on the surface of achromosomal and/or anucleate minicells, has agricultural, industrial, and environmental applications due to their improved stability durability and, reusability. Also, provided are methods for producing and purifying enzyme-immobilized minicells.

Abstract: A system monitors gas flow and pressure to a gas appliance in a fluid network comprising an analyzer. The analyzer has a housing defining an inlet, an outlet, and an interior in fluid communication with the inlet and the outlet. At least one sensor is coupled to the analyzer and configured to generate at least one signal related to gas being supplied to the gas appliance. A smart device communicates with the analyzer, wherein the smart device has a user interface and is configured to monitor, store and display data. The smart device can present any or all of a plurality of parameters such as the flow of gas, a capacity of the flow of gas, a temperature, a pressure of the gas and the like to a user based on signals from sensors.

Abstract: There is provided an inductive sensing circuit, comprising a signal generator, configured to generate a drive signal; one or more sensing arrangements, each of the one or more sensing arrangements comprising: two sets of one or more inductive sensing elements, configured in a half bridge arrangement, the two sets of one or more inductive sensing elements driven by the drive signal; a correction signal circuit, configured to generate a correction signal, wherein the correction signal is an adjustably scaled version of the drive signal; and a summing circuit, configured to sum an output signal of the two sets of one or more inductive sensing elements with the correction signal; and a demodulation circuit, configured to demodulate an output of the summing circuit of each of the one or more sensing arrangements.