Patents by Inventor George J. Christ
George J. Christ has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230357696Abstract: Disclosed herein are details of a hermetically or aseptically sealed bioreactor. The bioreactor comprises a bioreactor chamber, a membrane wall, a scaffold structure, a linear actuator, a linear transfer means, and a control system. Use of the bioreactor permits the inner scaffold structure to be moved and manipulated while still preserving a hermetic or aseptic seal inside the bioreactor chamber during operation.Type: ApplicationFiled: May 28, 2001Publication date: November 9, 2023Applicant: University of Virginia Patent FoundationInventors: George J. Christ, Gavin T. Garner
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Patent number: 11324857Abstract: Provided herein are methods of culturing organized skeletal muscle tissue from precursor muscle cells by cyclically stretching and relaxing said muscle cells on a support in vitro for a time sufficient to produce said organized skeletal muscle tissue, including reseeding said organized skeletal muscle tissue by contacting additional precursor muscle cells to said organized skeletal muscle tissue on said solid support, and then repeating said step of cyclically stretching and relaxing said muscle cells in said support in vitro for time sufficient to enhance the density (i.e., increased number of nuclei and/or number of multinucleated cells) of said organized skeletal muscle tissue on said support.Type: GrantFiled: December 14, 2016Date of Patent: May 10, 2022Assignee: Wake Forest University Health SciencesInventors: George J. Christ, Benjamin T. Corona
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Patent number: 11090410Abstract: Provided herein are scaffolds and methods useful to promote the formation of functional clusters on a tissue, for example, motor endplates (MEPs) or a component thereof on skeletal muscle cells or tissue, as well as the use of scaffolds so produced for repairing a tissue injury or defect.Type: GrantFiled: April 18, 2018Date of Patent: August 17, 2021Assignee: WAKE FOREST UNIVERSITY HEALTH SCIENCESInventors: George J. Christ, Justin M. Saul, John B. Scott, Benjamin T. Corona, Benjamin S. Harrison, Catherine Ward
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Publication number: 20180236131Abstract: Provided herein are scaffolds and methods useful to promote the formation of functional clusters on a tissue, for example, motor endplates (MEPs) or a component thereof on skeletal muscle cells or tissue, as well as the use of scaffolds so produced for repairing a tissue injury or defect.Type: ApplicationFiled: April 18, 2018Publication date: August 23, 2018Inventors: George J. Christ, Justin M. Saul, John B. Scott, Benjamin T. Corona, Benjamin S. Harrison, Catherine Ward
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Patent number: 9968705Abstract: Provided herein are scaffolds and methods useful to promote the formation of functional clusters on a tissue, for example, motor endplates (MEPs) or a component thereof on skeletal muscle cells or tissue, as well as the use of scaffolds so produced for repairing a tissue injury or defect.Type: GrantFiled: September 28, 2012Date of Patent: May 15, 2018Assignee: Wake Forest University Health SciencesInventors: George J. Christ, Justin M. Saul, John B Scott, Benjamin T. Corona, Benjamin S. Harrison, Catherine Ward
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Publication number: 20170087278Abstract: Provided herein are methods of culturing organized skeletal muscle tissue from precursor muscle cells by cyclically stretching and relaxing said muscle cells on a support in vitro for a time sufficient to produce said organized skeletal muscle tissue, including reseeding said organized skeletal muscle tissue by contacting additional precursor muscle cells to said organized skeletal muscle tissue on said solid support, and then repeating said step of cyclically stretching and relaxing said muscle cells in said support in vitro for time sufficient to enhance the density (i.e., increased number of nuclei and/or number of multinucleated cells) of said organized skeletal muscle tissue on said support.Type: ApplicationFiled: December 14, 2016Publication date: March 30, 2017Inventors: George J. Christ, Benjamin T. Corona
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Patent number: 9556418Abstract: Provided herein are methods of culturing organized skeletal muscle tissue from precursor muscle cells by cyclically stretching and relaxing said muscle cells on a support in vitro for a time sufficient to produce said organized skeletal muscle tissue, including reseeding said organized skeletal muscle tissue by contacting additional precursor muscle cells to said organized skeletal muscle tissue on said solid support, and then repeating said step of cyclically stretching and relaxing said muscle cells in said support in vitro for time sufficient to enhance the density (i.e., increased number of nuclei and/or number of multinucleated cells) of said organized skeletal muscle tissue on said support.Type: GrantFiled: February 12, 2013Date of Patent: January 31, 2017Assignee: Wake Forest University Health SciencesInventors: George J. Christ, Benjamin T. Corona
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Publication number: 20150366942Abstract: The invention provides methods of regulating smooth muscle tone in a subject, comprising the introduction, into smooth muscle cells of the subject, of a DNA sequence encoding a potassium channel protein involved in the regulation of smooth muscle tone, and expression of the DNA sequence in a sufficient number of smooth muscle cells of the subject to regulate smooth muscle tone in the subject. The invention provides methods of gene transfer for treating erectile dysfunction, bladder dysfunction, and other smooth muscle disorders.Type: ApplicationFiled: September 2, 2015Publication date: December 24, 2015Applicant: Albert Einstein College of Medicine of Yeshiva UniversityInventors: George J. Christ, Kelvin Davies, Arnold Melman
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Patent number: 9114196Abstract: Methods are provided for producing a bioscaffold from natural tissues by oxidizing a decellularized tissue to produce a bioscaffold having pores therein. The pore size and porosity is increased to better accommodate intact cells so that live cells can better infiltrate and inhabit the bioscaffold. The bioscaffold may be freeze-dried or lyophilized, sterilized and (optionally) aseptically packaged for subsequent use. A further aspect of the present invention is a bioscaffold produced by the processes described herein. Methods of treatment using the bioscaffold as a graft or as a biomedical implant for implantation are also provided. Also provided are methods of seeding a bioscaffold with mammalian cells, wherein the seeding carried out either in vitro or in vivo, and wherein a bioscaffold produced as described herein is utilized for said seeding.Type: GrantFiled: June 18, 2012Date of Patent: August 25, 2015Assignee: Wake Forest University Health SciencesInventors: Mark E. Van Dyke, George J. Christ, Patrick W. Whitlock
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Publication number: 20140234388Abstract: Provided herein are scaffolds and methods useful to promote the formation of functional clusters on a tissue, for example, motor endplates (MEPs) or a component thereof on skeletal muscle cells or tissue, as well as the use of scaffolds so produced for repairing a tissue injury or defect.Type: ApplicationFiled: September 28, 2012Publication date: August 21, 2014Applicant: Wake Forest University Health SciencesInventors: George J. Christ, Justin M. Saul, John B Scott, Benjamin T. Corona, Benjamin S. Harrison, Catherine Ward
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Publication number: 20140088176Abstract: The invention provides methods of regulating smooth muscle tone in a subject, comprising the introduction, into smooth muscle cells of the subject, of a DNA sequence encoding a potassium channel protein involved in the regulation of smooth muscle tone, and expression of the DNA sequence in a sufficient number of smooth muscle cells of the subject to regulate smooth muscle tone in the subject. The invention provides methods of gene transfer for treating erectile dysfunction, bladder dysfunction, and other smooth muscle disorders.Type: ApplicationFiled: March 18, 2013Publication date: March 27, 2014Applicant: Albert Einstein College of Medicine of Yeshiva UniversityInventors: George J. Christ, Kelvin Davies, Arnold Melman
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Publication number: 20120259415Abstract: Methods are provided for producing a bioscaffold from natural tissues by oxidizing a decellularized tissue to produce a bioscaffold having pores therein. The pore size and porosity is increased to better accommodate intact cells so that live cells can better infiltrate and inhabit the bioscaffold. The bioscaffold may be freeze-dried or lyophilized, sterilized and (optionally) aseptically packaged for subsequent use. A further aspect of the present invention is a bioscaffold produced by the processes described herein. Methods of treatment using the bioscaffold as a graft or as a biomedical implant for implantation are also provided. Also provided are methods of seeding a bioscaffold with mammalian cells, wherein the seeding carried out either in vitro or in vivo, and wherein a bioscaffold produced as described herein is utilized for said seeding.Type: ApplicationFiled: June 18, 2012Publication date: October 11, 2012Inventors: Mark E. Van Dyke, George J. Christ, Patrick W. Whitlock
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Patent number: 8221777Abstract: Methods are provided for producing a bioscaffold from natural tissues by oxidizing a decellularized tissue to produce a bioscaffold having pores therein. The pore size and porosity is increased to better accommodate intact cells so that live cells can better infiltrate and inhabit the bioscaffold. The bioscaffold may be freeze-dried or lyophilized, sterilized and (optionally) aseptically packaged for subsequent use. A further aspect of the present invention is a bioscaffold produced by the processes described herein. Methods of treatment using the bioscaffold as a graft or as a biomedical implant for implantation are also provided. Also provided are methods of seeding a bioscaffold with mammalian cells, wherein the seeding carried out either in vitro or in vivo, and wherein a bioscaffold produced as described herein is utilized for said seeding.Type: GrantFiled: October 1, 2010Date of Patent: July 17, 2012Assignee: Wake Forest University Health SciencesInventors: Mark E. Van Dyke, George J. Christ, Patrick W. Whitlock
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Publication number: 20110046732Abstract: Methods are provided for producing a bioscaffold from natural tissues by oxidizing a decellularized tissue to produce a bioscaffold having pores therein. The pore size and porosity is increased to better accommodate intact cells so that live cells can better infiltrate and inhabit the bioscaffold. The bioscaffold may be freeze-dried or lyophilized, sterilized and (optionally) aseptically packaged for subsequent use. A further aspect of the present invention is a bioscaffold produced by the processes described herein. Methods of treatment using the bioscaffold as a graft or as a biomedical implant for implantation are also provided. Also provided are methods of seeding a bioscaffold with mammalian cells, wherein the seeding carried out either in vitro or in vivo, and wherein a bioscaffold produced as described herein is utilized for said seeding.Type: ApplicationFiled: October 1, 2010Publication date: February 24, 2011Inventors: Mark E. Van Dyke, George J. Christ, Patrick W. Whitlock
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Patent number: 7829108Abstract: Methods are provided for producing a bioscaffold from natural tissues by oxidizing a decellularized tissue to produce a bioscaffold having pores therein. The pore size and porosity is increased to better accommodate intact cells so that live cells can better infiltrate and inhabit the bioscaffold. The bioscaffold may be freeze-dried or lyophilized, sterilized and (optionally) aseptically packaged for subsequent use. A further aspect of the present invention is a bioscaffold produced by the processes described herein. Methods of treatment using the bioscaffold as a graft or as a biomedical implant for implantation are also provided. Also provided are methods of seeding a bioscaffold with mammalian cells, wherein the seeding carried out either in vitro or in vivo, and wherein a bioscaffold produced as described herein is utilized for said seeding.Type: GrantFiled: April 20, 2007Date of Patent: November 9, 2010Assignee: Wake Forest University Health SciencesInventors: Mark E. Van Dyke, George J. Christ, Patrick W. Whitlock
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Publication number: 20090068152Abstract: The invention provides methods of regulating smooth muscle tone in a subject, comprising the introduction, into smooth muscle cells of the subject, of a DNA sequence encoding a potassium channel protein involved in the regulation of smooth muscle tone, and expression of the DNA sequence in a sufficient number of smooth muscle cells of the subject to regulate smooth muscle tone in the subject. The invention provides methods of gene transfer for treating erectile dysfunction, bladder dysfunction, and other smooth muscle disorders.Type: ApplicationFiled: November 23, 2004Publication date: March 12, 2009Applicant: ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIVERSITYInventors: George J. Christ, Kelvin Davies, Arnold Melman
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Publication number: 20070248638Abstract: Methods are provided for producing a bioscaffold from natural tissues by oxidizing a decellularized tissue to produce a bioscaffold having pores therein. The pore size and porosity is increased to better accommodate intact cells so that live cells can better infiltrate and inhabit the bioscaffold. The bioscaffold may be freeze-dried or lyophilized, sterilized and (optionally) aseptically packaged for subsequent use. A further aspect of the present invention is a bioscaffold produced by the processes described herein. Methods of treatment using the bioscaffold as a graft or as a biomedical implant for implantation are also provided. Also provided are methods of seeding a bioscaffold with mammalian cells, wherein the seeding carried out either in vitro or in vivo, and wherein a bioscaffold produced as described herein is utilized for said seeding.Type: ApplicationFiled: April 20, 2007Publication date: October 25, 2007Inventors: Mark E. Van Dyke, George J. Christ, Patrick W. Whitlock
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Patent number: 7030096Abstract: The present invention is directed towards a method of regulating smooth muscle tone, comprising the introduction, into smooth muscle cells of a subject, of a DNA sequence encoding a protein involved in the regulation of smooth muscle tone, and expression of the DNA sequence in a sufficient number of smooth muscle cells of the subject to regulate smooth muscle tone in the subject. Specifically, the invention provides methods of gene therapy for treating erectile dysfunction, bladder dysfunction, and other smooth muscle disorders. The present invention also provides recombinant viral and non-viral vectors comprising DNA encoding a protein involved in the regulation of smooth muscle tone. Further provided by the present invention is a smooth muscle cell which expresses a gene encoding a protein involved in the regulation of smooth muscle tone.Type: GrantFiled: March 21, 2000Date of Patent: April 18, 2006Assignee: Albert Einstein College of Medicine of Yeshiva UniversityInventors: Jan Geliebter, George J. Christ, Arnold Melman, Jamil Rehman
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Patent number: 6271211Abstract: The present invention is directed towards a method of regulating smooth muscle tone, comprising the introduction, into smooth muscle cells of a subject, of a DNA sequence encoding a protein involved in the regulation of smooth muscle tone, and expression of the DNA sequence in a sufficient number of smooth muscle cells of the subject to regulate smooth muscle tone in the subject. Specifically, the invention provides methods of gene therapy for treating erectile dysfunction, bladder dysfunction, and other smooth muscle disorders. The present invention also provides recombinant viral and non-viral vectors comprising DNA encoding a protein involved in the regulation of smooth muscle tone. Further provided by the present invention is a smooth muscle cell which expresses a gene encoding a protein involved in the regulation of smooth muscle tone.Type: GrantFiled: March 21, 2000Date of Patent: August 7, 2001Assignee: Albert Einstein College of Medicine of Yeshiva UniversityInventors: George J. Christ, Arnold Melman
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Patent number: 6239117Abstract: The present invention is directed towards a method of regulating smooth muscle tone, comprising the introduction, into smooth muscle cells of a subject, of a DNA sequence encoding a protein involved in the regulation of smooth muscle tone, and expression of the DNA sequence in a sufficient number of smooth muscle cells of the subject to regulate smooth muscle tone in the subject. Specifically, the invention provides methods of gene therapy for treating erectile dysfunction, bladder dysfunction, and other smooth muscle disorders. The present invention also provides recombinant viral and non-viral vectors comprising DNA encoding a protein involved in the regulation of smooth muscle tone. Further provided by the present invention is a smooth muscle cell which expresses a gene encoding a protein involved in the regulation of smooth muscle tone.Type: GrantFiled: March 21, 2000Date of Patent: May 29, 2001Assignee: Albert Einstein College of Medicine of Yeshiva UniversityInventors: George J. Christ, Arnold Melman