Abstract: A highly efficient method of making a primary cell derived biologic by purifying mononuclear cells (MNCs) in a automated cell processor to remove contaminating cells by loading leukocytes onto lymphocyte separation medium (LSM) and centrifuging the medium to obtain purified MNCs, storing the MNCs overnight in a closed sterile bag system, stimulating an induction mixture of the MNCs with phytohemagglutinin (PHA) or other mitogen and ciprofloxacin in a scalable cell culture device and producing a primary cell derived biologic from the MNCs, removing the mitogen from the induction mixture by filtering, incubating the induction mixture, clarifying the induction mixture by filtering to obtain a primary cell derived biologic supernatant, and clearing the primary cell derived biologic supernatant from adventitious agents by anion exchange chromatography, filtration. A closed system prevents contamination of the resulting primary cell derived biologic. An automated method of purifying cells.

Abstract: A highly efficient method of making a primary cell derived biologic by purifying mononuclear cells (MNCs) in a automated cell processor to remove contaminating cells by loading leukocytes onto lymphocyte separation medium (LSM) and centrifuging the medium to obtain purified MNCs, storing the MNCs overnight in a closed sterile bag system, stimulating an induction mixture of the MNCs with phytohemagglutinin (PHA) or other mitogen and ciprofloxacin in a scalable cell culture device and producing a primary cell derived biologic from the MNCs, removing the mitogen from the induction mixture by filtering, incubating the induction mixture, clarifying the induction mixture by filtering to obtain a primary cell derived biologic supernatant, and clearing the primary cell derived biologic supernatant from adventitious agents by anion exchange chromatography, filtration. A closed system prevents contamination of the resulting primary cell derived biologic. An automated method of purifying cells.

Abstract: A disposable sanitary gauge tee with no dead zone for use in pressure measurement of fluid in disposable tubing including a body having a main fluid passageway in fluid connection with a proximal end of a pressure fluid passageway, the main fluid passageway including a fluid port and a second fluid port, wherein a hose barb is operatively attached to the body at the fluid port and the second fluid port, wherein the disposable tubing can be removably attached to the hose barbs, and the pressure fluid passageway including a distal end for removable attachment of a sanitary pressure gauge and a biocompatible gauge protector for isolation of the fluid from the sanitary pressure gauge. No dead zone is achieved within the gauge tee as the proximal end of the pressure fluid passageway is substantially the same diameter as the distal end of the pressure fluid passageway, therein providing a total transfer of fluid within the pressure fluid passageway during fluid flow.