Abstract: The present invention relates functional ligands to target molecules, particularly to functional nucleic acids and modifications thereof, and to methods for simultaneously generating, for example, numerous different functional biomolecules, particularly to methods for generating numerous different functional nucleic acids against multiple target molecules simultaneously. The present invention further relates to functional ligands which bind with affinity to targets, such as viruses (e.g. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)), and/or antigens thereof (e.g. Spike protein and/or its subunits).

Abstract: This invention relates to methods for molecular detection, particularly to methods utilizing target-specific molecular probes. In exemplary embodiments, target-specific molecular probes include single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) aptamers. In general, the molecular probe may bind with relatively high specificity to a given target. In one aspect, a method for molecular detection comprises a molecular probe paired to a reporter molecule wherein the molecular probe impairs the amplification of the reporter molecule in the absence of the target molecule.

Abstract: This invention relates to methods for molecular detection, particularly to methods utilizing target-specific molecular probes. In exemplary embodiments, target-specific molecular probes include single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) aptamers. In general, the molecular probe may bind with relatively high specificity to a given target. In one aspect, a method for molecular detection comprises a molecular probe paired to a reporter molecule wherein the molecular probe impairs the amplification of the reporter molecule in the absence of the target molecule.

Abstract: The present invention relates to methods for generating functional biomolecules. In one exemplary aspect of the invention, generation of functional biomolecules may be performed against multiple targets simultaneously within a single system. In general, a plurality of targets may be disposed within in a single reaction volume and a library of biomolecules, such as a nucleic acid library, may be applied to the reaction volume. The members of the library that do not bind to any of the plurality of targets under given conditions may then be partitioned. The remaining members of the library may then be marked and/or tagged, such as to identify the particular target or targets to which the member of the library binds. The binding members of the library may then be isolated and, by virtue of the marking or tagging, be matched to a particular target or targets.

Abstract: The present invention is directed to devices and methods for molecular association, particularly to devices and methods for hybridization of nucleic acids utilizing temperature gradients and imaging thereof. In one aspect, a molecular hybridization system generally includes a substrate having a plurality of molecular probes attached thereto, the plurality of probes being generally present in multiple copies arranged in localized formations on the surface of the substrate. The molecular hybridization system further generally includes a chamber that encloses the plurality of molecular probes such that a fluid containing sample may be applied and kept in contact with the substrate having the probes thereon. The molecular hybridization system also includes a temperature affecting system that generally produces at least one desired temperature on the surface of the substrate and in the adjacent fluid within the chamber.

Abstract: This invention relates to methods for molecular detection, particularly to methods utilizing target-specific molecular probes. In exemplary embodiments, target-specific molecular probes include single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) aptamers. In general, the molecular probe may bind with relatively high specificity to a given target. In one aspect, a method for molecular detection comprises a molecular probe paired to a reporter molecule wherein the molecular probe impairs the amplification of the reporter molecule in the absence of the target molecule.

Abstract: This invention relates to methods for molecular detection, particularly to methods utilizing target-specific molecular probes. In exemplary embodiments, target-specific molecular probes include single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) aptamers. In general, the molecular probe may bind with relatively high specificity to a given target. In one aspect, a method for molecular detection comprises a molecular probe paired to a reporter molecule wherein the molecular probe impairs the amplification of the reporter molecule in the absence of the target molecule.

Abstract: This invention relates to methods for molecular detection, particularly to methods utilizing target-specific molecular probes. In exemplary embodiments, target-specific molecular probes include single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) aptamers. In general, the molecular probe may bind with relatively high specificity to a given target. In one aspect, a method for molecular detection comprises a molecular probe paired to a reporter molecule wherein the molecular probe impairs the amplification of the reporter molecule in the absence of the target molecule.

Abstract: The present invention relates to methods for generating functional biomolecules, particularly to methods for generating multiple functional nucleic acids against multiple target molecules simultaneously. The present invention further relates to methods for generating functional biomolecules, particularly to functional nucleic acids, that bind with functional activity to another biomolecule, such as a receptor molecule. In one exemplary aspect of the invention, generation of functional biomolecules may be performed against multiple targets simultaneously within a single system, such as the generation of functional nucleic acid ligands within a single reaction volume. In general, a plurality of targets may be disposed within in a single reaction volume and a library of biomolecules, such as a nucleic acid library, may be applied to the reaction volume. The members of the library that do not bind to any of the plurality of targets under given conditions may then be partitioned, such as by washing.

Abstract: The present invention relates to methods of generating amounts of selective nucleic acids. The present invention further relates to selective nucleic acids incorporated within non-coding nucleic acids, capable of binding to or altering a target molecule. Selective nucleic acids may generally refer to, but are not limited to, deoxyribonucleic acids (DNAs), ribonucleic acids (RNAs), artificially modified nucleic acids, combinations or modifications thereof. Selective nucleic acids may also generally refer to, but are not limited to, nucleic acid aptamers, aptazymes, ribozymes, deoxyribozymes, nucleic acid probes, small interfering RNAs (siRNAs), micro RNAs (miRNAs), short hairpin RNAs (shRNAs), antisense nucleic acids, diagnostic probes or probe libraries, aptamer inhibitors, precursors of any of the above and/or combinations or modifications thereof. In one aspect, a method for generating amounts of selective nucleic acids includes incorporating a selective nucleic acid sequence into a carrier nucleic acid.

Abstract: This invention relates to methods for molecular detection, particularly to methods utilizing target-specific molecular probes. In exemplary embodiments, target-specific molecular probes include single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) aptamers. In general, the molecular probe may bind with relatively high specificity to a given target. In one aspect, a method for molecular detection comprises a molecular probe paired to a reporter molecule wherein the molecular probe impairs the amplification of the reporter molecule in the absence of the target molecule.

Abstract: The present invention is directed to nucleic acids with biomimetic properties and methods for producing said nucleic acids. In particular, this invention relates to nucleic acids exhibiting biomimetic properties in relation to proteins such as growth factors, hormones and/or other cell signaling proteins. Biomimetic properties may generally be defined as interactive ability in the same and/or similar manner as another biological molecule. This may, for example, include interacting with a ligand-binding biomolecule, such as a cell signaling receptor, in a manner similar to a native ligand. In the case of a signaling receptor, such biomimetic nucleic acids may in general act as an agonist or an antagonist to the given receptor. They may further act in competition to a native ligand.

Abstract: The present invention is directed to devices with enhanced biocompatibility and methods for generating and utilizing such devices. The present invention is further directed to enhanced biocompatibility utilizing oligonucleotide functionalization. In one aspect, a device for implantation and/or prolonged exposure to the body tissues includes a functionalized surface. The functionalized surface generally enhances the biocompatibility of the device with body tissues. In some embodiments, the functionalized surface includes substances for controlling interaction between the device and the body tissues. Substances for controlling interactions may include, but are not limited to, polymeric materials, biomolecules, ions and/or ion-releasing substances, and/or any other appropriate substance or combination thereof. In exemplary embodiments, the functionalized surface includes oligonucleotides for controlling interaction between the device and the body tissues.

Abstract: The present invention is directed to devices and methods for molecular association, particularly to devices and methods for hybridization of nucleic acids utilizing temperature gradients and imaging thereof. In one aspect, a molecular hybridization system generally includes a substrate having a plurality of molecular probes attached thereto, the plurality of probes being generally present in multiple copies arranged in localized formations on the surface of the substrate. The molecular hybridization system further generally includes a chamber that encloses the plurality of molecular probes such that a fluid containing sample may be applied and kept in contact with the substrate having the probes thereon. The molecular hybridization system also includes a temperature affecting system that generally produces at least one desired temperature on the surface of the substrate and in the adjacent fluid within the chamber.

Abstract: A column mounted shoring bracket has a support sub-assembly attached to a column or other supporting surface, a jack sub-assembly attached to the support and a head sub-assembly attached to a part of the jack that varies in height relative to the support. The head has a head base that supports one or more rollers for moving a form. The head also has a supporting plate for supporting the form. The supporting plate is connected to the head base so that it may be slid upwards and fixed in a position where the top of the supporting plate is above the top of the rollers, for example to carry the weight of a slab being built, or slid downwards so that the top of the supporting plate is below the top of the rollers which do not have to carry the weight of the slab being built. The angular position of the rollers may be aligned with an external reference such as the side of a column, a wall, or a jig.

Abstract: A method is disclosed which decreases the amount of error correction code data required to detect and correct errors in digital data while still maintaining a specified ability to correct errors in large groups of contiguous data. The present invention accomplishes this by placing distance either in space or in time between symbols grouped mathematically for error correction code calculations. An error affecting contiguous symbols, like scratches or other defects in digital storage media, or certain types of interference in either wired or wireless digital communications, would affect many error correction code groups, but would only affect one symbol from each group. The error can easily be corrected using only a one dimensional error correction code, removing the need for a two dimensional product error correction code and the additional data overhead that the column ECC data necessarily adds to the block.

Abstract: A method of making a composite roofing material and the resulting material by depositing nail tabs made of a thermoplastic, thermosetting, adhesive or elastomer material, in a liquid state, onto the base substrate of the composite roofing material or onto the saturated or coated roofing material, or onto a transfer surface to be pressed or laminated onto the roofing material. A preferred embodiment transfers the nail tabs onto an engraved transfer impression roll and uses a pressurized applicator to inject the viscous tab material into engraved patterns depressed in raised areas of the impression roll., then deposits the material onto the roofing material. A preferred embodiment also includes the thermoplastic or thermosetting material in a liquid or viscous state hardened or cured by either its exposure to the air or by the use of ultra-violet or visible light.

Abstract: Process for space time detection with the past, present and future using factual real life objects, actions and events allowing for desired actions. The process can run on any chosen computer system or software using file or database formats containing the object information or can be processed with human intellect without computers. Information is entered into a file or database system with automated or manual methods storing the date and time of the objects being people, places and things. The objects locations and surroundings are mapped and stored. Information about the objects are obtained and if not on file they are entered. A recognition process is then used to decode variations of the object information and written to storage. As each record is stored an automated monitoring, reporting alarm and analysis process is then available for human or computer analysis allowing for any preprogrammed or chosen action.

Abstract: An overhang bracket has a top member, a side member and a diagonal member. The side and top member may be connected together through a removable connecter. A guardrail post holder allows a guardrail post to be installed at a variety of angles to the top member. Rotating the side member causes the diagonal member to translate up or down the side member. A side member locator extends upwards from the overhang bracket to indicate the location of the side member. A tie rod holder holds a tie rod in an offset position relative to the side member. The side member locator may extend upwards through further parts of the shoring system or bridge overhang structure, for example a floor of a concrete form or rebar. A worker may adjust the overhang bracket in place from a standing position on a supporting structure or the floor of the form.

Abstract: Identification of microorganisms based on the sequences of their 5S, 23S and particularly 16S ribosomal RNAs is growing in utility as the database of known ribosomal RNA sequences expands. Experimental identification is usually based on matching the experimentally-determined sequence of an organisms rRNA to a previously-determined sequence in the databank, or hybridization of the organisms rRNA or encoding rDNA to an oligonucleotide probe specific for an organism anticipated to be present in the sample. Here we propose the identification of microorganisms based on the overall composition (not sequence or hybridization propensity) of characteristic molecules derived from their rRNA or rDNA sequences by enzymatic cleavage or localized amplification. Ribonuclease T1 fragments of rRNA composition determination by mass spectrometry are especially favored.