Abstract: Sacchromyces cerevisiae strains modified to express an adhE (alcohol/aldehyde dehydrogenase) enzyme having the amino acid sequence SEQ ID NO:1, or a functional variant thereof capable of catalysing the conversion of acetyl CoA to ethanol, and/or to overexpress an ACS2 (acetyl-CoA synthetase) enzyme having the amino acid sequence SEQ ID NO:2, or a functional variant thereof capable of catalysing the conversion of acetate to acetyl CoA. Such cells have an improved ability to grow on or in acetate-containing media compared to cells not according to the invention.

Abstract: A method for preparing a hydrocarbon comprising contacting a fatty acid substrate with at least one fatty acid reductase and at least one fatty aldehyde synthetase and at least one fatty acyl transferase, wherein the fatty acid substrate is a fatty acid, a fatty acyl-ACP, or a fatty acyl-CoA or a mixture of any of these, to obtain a fatty aldehyde; and contacting the fatty aldehyde with at least one aldehyde decarbonylase enzyme.

Abstract: A diesel fuel formulation containing a triethoxypropane and a palm oil methyl ester (POME), and a diesel fuel supplement containing a premix of a triethoxypropane and a POME is disclosed. The triethoxypropane may be 1,2,3-triethoxypropane or 1,1,3-triethoxypropane.

Abstract: A diesel fuel formulation with improved lubricity is provided containing (i) a fatty alcohol ester, (ii) an acid-based lubricity additive and (iii) an additional diesel fuel component. The ester (i) has the formula R1-C(O)—O—R2, in which R1 is for example either hydrogen or methyl and R2 is for example a C6 to C14 alkyl group.

Abstract: A fuel composition comprising: a diesel base fuel; from 1 to 10% v/v of a fatty acid alkyl ester; and more than 10% v/v of an ether component, the ether component comprising one or more ether compounds having in the range of from 8 to 12 carbon atoms and selected from compounds of formula I R1—O—R2??(I) wherein R1 and R2 are independently primary or secondary alkyl.

Abstract: Method for converting a carbon source such as a plant-derived material into a hydrocarbon or hydrocarbon precursor, via a microorganism-catalysed aerobic reaction. The microorganism is cultivated in the presence of the carbon source and of oxygen, in a reaction vessel having a capacity of about 2000 litres or greater which comprises an aeration system for supplying oxygen to the microorganism. Also provided is an apparatus for use in the method, comprising a reaction vessel of capacity 2000 litres or greater, and an aeration system.

Abstract: Gasoline fuel formulation containing (i) tricyclene, which is suitably biologically derived, and (ii) a gasoline base fuel. The formulation may contain one or more additional biofuel components or oxygenates: it may for example include ethanol, or ethanol together with one or more additional biofuels. The invention also provides a method for preparing the formulation, its use in a spark ignition engine, and the use of tricyclene in a gasoline fuel formulation for various purposes, including to enhance lubricity.

Abstract: A diesel fuel formulation containing a triethoxypropane and a palm oil methyl ester (POME), and a diesel fuel supplement containing a premix of a triethoxypropane and a POME is disclosed. The triethoxypropane may be 1,2,3-triethoxypropane or 1,1,3-triethoxypropane.

Abstract: Gasoline fuel formulation containing (i) tricyclene, which is suitably biologically derived, and (ii) a gasoline base fuel. The formulation may contain one or more additional biofuel components or oxygenates: it may for example include ethanol, or ethanol together with one or more additional biofuels. The invention also provides a method for preparing the formulation, its use in a spark ignition engine, and the use of tricyclene in a gasoline fuel formulation for various purposes, including to enhance lubricity.

Abstract: A diesel fuel formulation with improved lubricity is provided containing (i) a fatty alcohol ester, (ii) an acid-based lubricity additive and (iii) an additional diesel fuel component. The ester (i) has the formula R1-C(O)—O—R2, in which R1 is for example either hydrogen or methyl and R2 is for example a C6 to C14 alkyl group.

Abstract: A fuel composition containing a major amount of a fuel for an internal combustion engine and a minor amount of a fuel-compatible dendrimer containing from 4 to 64 terminal functional groups independently selected from amino, hydroxyl and carboxylate groups is disclosed. The use of such compositions as fuel in an internal combustion engine for controlling combustion chamber deposits is also disclosed.