Abstract: A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.

Abstract: An enclosure installation assembly comprises a support base and a mounting pad adapted to be secured to the support base. The support base, having a lower portion slightly larger than its upper portion, is placed in a hole in the ground. Dirt is compacted around the support base to secure the support base in place. The mounting pad is then secured to the support base using support braces that internally engage the support base and fasteners that engage the mounting pad. Finally, an enclosure is installed on the mounting pad, with fasteners located inside the enclosure. The resulting enclosure is securely and easily installed, and is tamper resistant.