Abstract: The presently disclosed subject matter provides a process for starch liquefaction using at least two classes of ?-amylase enzymes, wherein the starch hydrolysis pattern from at least two of these classes is different. At least one class of enzyme is provided to the liquefaction process in the form of transgenic plant material expressing at least one class of ?-amylase enzyme or is provided in the form of a purified or partially-purified ?-amylase enzyme preparation. The second or subsequent class(es) of ?-amylase enzymes may be provided in the form of additional transgenic plant material expressing the second or subsequent class(es), or may be provided in the form of a second or subsequent purified or partially-purified ?-amylase enzyme preparation.

Abstract: The presently disclosed subject matter provides a process for starch liquefaction using at least two classes of ?-amylase enzymes, wherein the starch hydrolysis pattern from at least two of these classes is different. At least one class of enzyme is provided to the liquefaction process in the form of transgenic plant material expressing at least one class of ?-amylase enzyme or is provided in the form of a purified or partially-purified ?-amylase enzyme preparation. The second or subsequent class(es) of ?-amylase enzymes may be provided in the form of additional transgenic plant material expressing the second or subsequent class(es), or may be provided in the form of a second or subsequent purified or partially-purified ?-amylase enzyme preparation.

Abstract: The presently disclosed subject matter provides a process for starch liquefaction using at least two classes of ?-amylase enzymes, wherein the starch hydrolysis pattern from at least two of these classes is different. At least one class of enzyme is provided to the liquefaction process in the form of transgenic plant material expressing at least one class of ?-amylase enzyme or is provided in the form of a purified or partially-purified ?-amylase enzyme preparation. The second or subsequent class(es) of ?-amylase enzymes may be provided in the form of additional transgenic plant material expressing the second or subsequent class(es), or may be provided in the form of a second or subsequent purified or partially-purified ?-amylase enzyme preparation.

Abstract: The presently disclosed subject matter provides a process for starch liquefaction using at least two classes of ?-amylase enzymes, wherein the starch hydrolysis pattern from at least two of these classes is different. At least one class of enzyme is provided to the liquefaction process in the form of transgenic plant material expressing at least one class of ?-amylase enzyme or is provided in the form of a purified or partially-purified ?-amylase enzyme preparation. The second or subsequent class(es) of ?-amylase enzymes may be provided in the form of additional transgenic plant material expressing the second or subsequent class(es), or may be provided in the form of a second or subsequent purified or partially-purified ?-amylase enzyme preparation.

Abstract: The presently disclosed subject matter provides a process for starch liquefaction using at least two classes of ?-amylase enzymes, wherein the starch hydrolysis pattern from at least two of these classes is different. At least one class of enzyme is provided to the liquefaction process in the form of transgenic plant material expressing at least one class of ?-amylase enzyme or is provided in the form of a purified or partially-purified ?-amylase enzyme preparation. The second or subsequent class(es) of ?-amylase enzymes may be provided in the form of additional transgenic plant material expressing the second or subsequent class(es), or may be provided in the form of a second or subsequent purified or partially-purified ?-amylase enzyme preparation.

Abstract: The presently disclosed subject matter provides a process for starch liquefaction using at least two classes of ?-amylase enzymes, wherein the starch hydrolysis pattern from at least two of these classes is different. At least one class of enzyme is provided to the liquefaction process in the form of transgenic plant material expressing at least one class of ?-amylase enzyme or is provided in the form of a purified or partially-purified ?-amylase enzyme preparation. The second or subsequent class(es) of ?-amylase enzymes may be provided in the form of additional transgenic plant material expressing the second or subsequent class(es), or may be provided in the form of a second or subsequent purified or partially-purified ?-amylase enzyme preparation.

Abstract: The presently disclosed subject matter provides a process for starch liquefaction using at least two classes of ?-amylase enzymes, wherein the starch hydrolysis pattern from at least two of these classes is different. At least one class of enzyme is provided to the liquefaction process in the form of transgenic plant material expressing at least one class of ?-amylase enzyme or is provided in the form of a purified or partially-purified ?-amylase enzyme preparation. The second or subsequent class(es) of ?-amylase enzymes may be provided in the form of additional transgenic plant material expressing the second or subsequent class(es), or may be provided in the form of a second or subsequent purified or partially-purified ?-amylase enzyme preparation.

Abstract: The presently disclosed subject matter provides a process for starch liquefaction using at least two classes of ?-amylase enzymes, wherein the starch hydrolysis pattern from at least two of these classes is different. At least one class of enzyme is provided to the liquefaction process in the form of transgenic plant material expressing at least one class of ?-amylase enzyme or is provided in the form of a purified or partially-purified ?-amylase enzyme preparation. The second or subsequent class(es) of ?-amylase enzymes may be provided in the form of additional transgenic plant material expressing the second or subsequent class(es), or may be provided in the form of a second or subsequent purified or partially-purified ?-amylase enzyme preparation.

Abstract: The presently disclosed subject matter provides a process for starch liquefaction using at least two classes of ?-amylase enzymes, wherein the starch hydrolysis pattern from at least two of these classes is different. At least one class of enzyme is provided to the liquefaction process in the form of transgenic plant material expressing at least one class of ?-amylase enzyme or is provided in the form of a purified or partially-purified ?-amylase enzyme preparation. The second or subsequent class(es) of ?-amylase enzymes may be provided in the form of additional transgenic plant material expressing the second or subsequent class(es), or may be provided in the form of a second or subsequent purified or partially-purified ?-amylase enzyme preparation.

Abstract: The presently disclosed subject matter provides improved processes for processing starch from plant sources, including processes for starch liquefaction, for simultaneous liquefaction and saccharification, and for the preparation of ethanol. These processes can be performed without a pH adjustment and at relatively low temperatures. The processes can involve the use of starch-containing plant material derived from plants that express starch-digesting enzymes. The presently disclosed subject matter further relates to improved processes for the preparation of other starch-derived products, including dried distiller grain (dried distiller grain) and dried distiller grain and solubles (dried distiller grain and solubles), and to the starch-derived products, themselves.

Abstract: The presently disclosed subject matter provides a process for starch liquefaction using at least two classes of ?-amylase enzymes, wherein the starch hydrolysis pattern from at least two of these classes is different. At least one class of enzyme is provided to the liquefaction process in the form of transgenic plant material expressing at least one class of a-amylase enzyme or is provided in the form of a purified or partially-purified ?-amylase enzyme preparation. The second or subsequent class(es) of ?-amylase enzymes may be provided in the form of additional transgenic plant material expressing the second or subsequent class(es), or may be provided in the form of a second or subsequent purified or partially-purified ?-amylase enzyme preparation.

Abstract: The presently disclosed subject matter provides improved processes for processing starch from plant sources, including processes for starch liquefaction, for simultaneous liquefaction and saccharification, and for the preparation of ethanol. These processes can be performed without a pH adjustment and at relatively low temperatures. The processes can involve the use of starch-containing plant material derived from plants that express starch-digesting enzymes. The presently disclosed subject matter further relates to improved processes for the preparation of other starch-derived products, including dried distiller grain (dried distiller grain) and dried distiller grain and solubles (dried distiller grain and solubles), and to the starch-derived products, themselves.