Patents by Inventor Georges Pignede

Georges Pignede has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11473072
    Abstract: Strains of Saccharomyces cerevisiae yeast that are genetically modified so as to co-express a gene coding a glucoamylase of fungal origin, a gene coding a glucoamylase of Saccharomyces cerevisiae var. diastaticus, and a gene coding a xylanase of fungal origin. The production yield of bioethanol through these strains is greater than that of strains that are otherwise identical but that do not include the gene coding the xylanase of fungal origin. Also, a method for obtaining these yeasts, as well as the use of these yeasts in the production of bioethanol.
    Type: Grant
    Filed: July 2, 2019
    Date of Patent: October 18, 2022
    Assignee: LESAFFRE ET COMPAGNIE
    Inventors: Georges Pignede, Maud Petit, Benoît Thomas, Sébastien Hulin
  • Patent number: 11345936
    Abstract: The invention relates to a genetically modified fungal microorganism for the production of frambinone, the microorganism having the following characteristics: —the capacity to produce frambinone from tyrosine; and —a limited capacity or no capacity to break tyrosine down into tyrosol, p-hydroxyphenylacetaldehyde and/or p-hydroxyphenylacetate; and to the use of same for producing frambinone.
    Type: Grant
    Filed: August 27, 2020
    Date of Patent: May 31, 2022
    Assignee: LESAFFRE et COMPAGNIE
    Inventors: Alexander Matthias Farwick, Thomas Desfougeres, Georges Pignede, Anaïs Roussel, Isabelle Mouly, Brieuc Morvan
  • Patent number: 11186814
    Abstract: The invention relates to a method for selecting a yeast strain having improved capability for fermenting a pentose, advantageously xylose, in the presence of organic acid, advantageously acetic acid, in non-dissociated form, in which at least one yeast strain that is capable of fermenting said pentose is consecutively cultured in the following two media: a first growth medium comprising said pentose as the only carbon source and said organic acid in non-dissociated form; a second growth medium comprising another carbon source as the only carbon source, advantageously glucose, free of said organic acid in non-dissociated form, the consecutive culture in at least said two growth media being repeated at least twice, in the presence of rising concentrations of organic acid in non-dissociated form.
    Type: Grant
    Filed: February 18, 2020
    Date of Patent: November 30, 2021
    Assignee: LESAFFRE et COMPAGNIE
    Inventors: Jean-Michel Bavouzet, Thomas Desfougeres, Georges Pignede, Jennifer Techel
  • Publication number: 20210261934
    Abstract: Strains of Saccharomyces cerevisiae yeast that are genetically modified so as to co-express a gene coding a glucoamylase of fungal origin, a gene coding a glucoamylase of Saccharomyces cerevisiae var. diastaticus, and a gene coding a xylanase of fungal origin. The production yield of bioethanol through these strains is greater than that of strains that are otherwise identical but that do not include the gene coding the xylanase of fungal origin. Also, a method for obtaining these yeasts, as well as the use of these yeasts in the production of bioethanol.
    Type: Application
    Filed: July 2, 2019
    Publication date: August 26, 2021
    Applicant: LESAFFRE ET COMPAGNIE
    Inventors: Georges PIGNEDE, Maud PETIT, Benoît THOMAS, Sébastien HULIN
  • Patent number: 10947519
    Abstract: The present application relates to improved Saccharomyces cerevisiae yeast strains that co-express a gene encoding a glucoamylase of fungal origin and a gene encoding the glucoamylase of Saccharomyces cerevisiae var. diastaticus. The present invention also relates to a method for obtaining these yeast strains involving a) genetically modifying a Saccharomyces cerevisiae yeast to co-express a gene encoding a glucoamylase of fungal origin and a gene encoding the glucoamylase of Saccharomyces cerevisiae var. diastaticus; b) culturing and fermenting the strain obtained in step a) on a dextrin medium; c) selecting the strains having fermentation kinetics at least equal to or greater than those obtained with the strain deposited at the CNCM [French National Collection of Microorganism Cultures] under number I 4999 under the same conditions. The yeast strains according to the invention are of particular interest in producing bioethanol.
    Type: Grant
    Filed: August 23, 2016
    Date of Patent: March 16, 2021
    Assignee: LESAFFRE ET COMPAGNIE
    Inventors: Maud Petit, Georges Pignede, Jean-Michel Bavouzet, Benoît Thomas
  • Patent number: 10920232
    Abstract: The present invention relates to methods for obtaining yeast strains suitable for metabolizing arabinose, and to improved strains with good performance as regards their capacity to ferment arabinose as well as xylose and glucose, including in the presence of inhibitors such as acetic acid.
    Type: Grant
    Filed: January 24, 2018
    Date of Patent: February 16, 2021
    Assignee: LESAFFRE et COMPAGNIE
    Inventors: Thomas Desfougeres, Emilie Fritsch, Georges Pignede, Christophe Rave, Claire Thorel
  • Publication number: 20200392545
    Abstract: The invention relates to a genetically modified fungal microorganism for the production of frambinone, said microorganism having the following characteristics: —the capacity to produce frambinone from tyrosine; and —a limited capacity or no capacity to break tyrosine down into tyrosol, p-hydroxyphenylacetaldehyde and/or p-hydroxyphenylacetate; and to the use of same for producing frambinone.
    Type: Application
    Filed: August 27, 2020
    Publication date: December 17, 2020
    Inventors: Alexander Matthias Farwick, Thomas Desfougeres, Georges Pignede, Anaïs Roussel, Isabelle Mouly, Brieuc Morvan
  • Patent number: 10793880
    Abstract: The invention relates to a genetically modified fungal microorganism for the production of frambinone, the microorganism having the following characteristics: —the capacity to produce frambinone from tyrosine; and —a limited capacity or no capacity to break tyrosine down into tyrosol, p-hydroxyphenylacetaldehyde and/or p-hydroxyphenylacetate; and to the use of same for producing frambinone.
    Type: Grant
    Filed: June 2, 2017
    Date of Patent: October 6, 2020
    Assignee: LESAFFRE et COMPAGNIE
    Inventors: Alexander Matthias Farwick, Thomas Desfougeres, Georges Pignede, Anaïs Roussel, Isabelle Mouly, Brieuc Morvan
  • Patent number: 10738329
    Abstract: The invention concerns the use of the MCM7 gene to confer resistance to an organic acid, preferably acetic acid, to a yeast strain during glucose fermentation.
    Type: Grant
    Filed: August 31, 2017
    Date of Patent: August 11, 2020
    Assignee: LESAFFRE et COMPAGNIE
    Inventors: Georges Pignede, Thomas Desfougeres
  • Publication number: 20200208098
    Abstract: The invention relates to a method for selecting a yeast strain having improved capability for fermenting a pentose, advantageously xylose, in the presence of organic acid, advantageously acetic acid, in non-dissociated form, in which at least one yeast strain that is capable of fermenting said pentose is consecutively cultured in the following two media: a first growth medium comprising said pentose as the only carbon source and said organic acid in non-dissociated form; a second growth medium comprising another carbon source as the only carbon source, advantageously glucose, free of said organic acid in non-dissociated form, the consecutive culture in at least said two growth media being repeated at least twice, in the presence of rising concentrations of organic acid in non-dissociated form.
    Type: Application
    Filed: February 18, 2020
    Publication date: July 2, 2020
    Inventors: Jean-Michel Bavouzet, Thomas Desfougeres, Georges Pignede, Jennifer Techel
  • Patent number: 10604735
    Abstract: The invention relates to a method for selecting a yeast strain having improved capability for fermenting a pentose, advantageously xylose, in the presence of organic acid, advantageously acetic acid, in non-dissociated form, in which at least one yeast strain that is capable of fermenting said pentose is consecutively cultured in the following two media: a first growth medium comprising said pentose as the only carbon source and said organic acid in non-dissociated form; a second growth medium comprising another carbon source as the only carbon source, advantageously glucose, free of said organic acid in non-dissociated form, the consecutive culture in at least said two growth media being repeated at least twice, in the presence of rising concentrations of organic acid in non-dissociated form.
    Type: Grant
    Filed: April 27, 2016
    Date of Patent: March 31, 2020
    Assignee: LESAFFRE et COMPAGNIE
    Inventors: Jean-Michel Bavouzet, Thomas Desfougeres, Georges Pignede, Jennifer Techel
  • Publication number: 20190367951
    Abstract: The invention concerns the use of the MCM7 gene to confer resistance to an organic acid, preferably acetic acid, to a yeast strain during glucose fermentation.
    Type: Application
    Filed: August 31, 2017
    Publication date: December 5, 2019
    Inventors: Georges Pignede, Thomas Desfougeres
  • Publication number: 20190330645
    Abstract: The present invention relates to methods for obtaining yeast strains suitable for metabolizing arabinose, and to improved strains with good performance as regards their capacity to ferment arabinose as well as xylose and glucose, including in the presence of inhibitors such as acetic acid.
    Type: Application
    Filed: January 24, 2018
    Publication date: October 31, 2019
    Inventors: Thomas Desfougeres, Emilie Fritsch, Georges Pignede, Christophe Rave, Claire Thorel
  • Publication number: 20190309330
    Abstract: The invention relates to a genetically modified fungal microorganism for the production of frambinone, said microorganism having the following characteristics: —the capacity to produce frambinone from tyrosine; and —a limited capacity or no capacity to break tyrosine down into tyrosol, p-hydroxyphenylacetaldehyde and/or p-hydroxyphenylacetate; and to the use of same for producing frambinone.
    Type: Application
    Filed: June 7, 2017
    Publication date: October 10, 2019
    Inventors: Alexander Matthias Farwick, Thomas Desfougeres, Georges Pignede, Anaïs Roussel, Isabelle Mouly, Brieuc Morvan
  • Publication number: 20190292532
    Abstract: The present application relates to improved Saccharomyces cerevisiae yeast strains that co-express a gene encoding a glucoamylase of fungal origin and a gene encoding the glucoamylase of Saccharomyces cerevisiae var. diastaticus. The present invention also relates to a method for obtaining these yeast strains involving a) genetically modifying a Saccharomyces cerevisiae yeast to co-express a gene encoding a glucoamylase of fungal origin and a gene encoding the glucoamylase of Saccharomyces cerevisiae var. diastaticus; b) culturing and fermenting the strain obtained in step a) on a dextrin medium; c) selecting the strains having fermentation kinetics at least equal to or greater than those obtained with the strain deposited at the CNCM [French National Collection of Microorganism Cultures] under number I 4999 under the same conditions. The yeast strains according to the invention are of particular interest in producing bioethanol.
    Type: Application
    Filed: August 23, 2016
    Publication date: September 26, 2019
    Inventors: Maud PETIT, Georges PIGNEDE, Jean-Michel BAVOUZET, Benoît THOMAS
  • Patent number: 10273447
    Abstract: The present invention relates to a method for obtaining a strain capable of efficiently propagating in a low nutritive potential medium, capable of metabolizing pentoses and of resisting fermentation inhibitors, comprising the following steps; a) growth of a strain of recombinant yeast with a strain of wild yeast lacking any impairments, the recombinant yeast strain comprising at least one copy of an exogenous gene of xylose isomerase and at least one additional copy of a gene of D-xylulokinase included in the genome and linked to a single sexual characteristic of the strain, b) at least two cycles of genome shuffling by sporulation and or random hybridization, c) selection of the population obtained in step b) according to a suitability criterion of the strains to metabolize xylose, d) selection of the population obtained in step c) according to a suitability criterion of the strains to grow in a Pref type medium, a medium with low nutritive value.
    Type: Grant
    Filed: February 16, 2015
    Date of Patent: April 30, 2019
    Assignee: LESAFFRE et COMPAGNIE
    Inventors: Thomas Desfougeres, Georges Pignede, Jennifer Techel
  • Publication number: 20180155674
    Abstract: The invention relates to a method for selecting a yeast strain having improved capability for fermenting a pentose, advantageously xylose, in the presence of organic acid, advantageously acetic acid, in non-dissociated form, in which at least one yeast strain that is capable of fermenting said pentose is consecutively cultured in the following two media: a first growth medium comprising said pentose as the only carbon source and said organic acid in non-dissociated form; a second growth medium comprising another carbon source as the only carbon source, advantageously glucose, free of said organic acid in non-dissociated form, the consecutive culture in at least said two growth media being repeated at least twice, in the presence of rising concentrations of organic acid in non-dissociated form.
    Type: Application
    Filed: April 27, 2016
    Publication date: June 7, 2018
    Inventors: Jean-Michel Bavouzet, Thomas Desfougeres, Georges Pignede, Jennifer Techel
  • Patent number: 9872504
    Abstract: The present invention relates to novel broad spectrum strains of baker's yeast, i.e. yeast strains that are effective on both dough known as unsweetened dough and dough known as sweet dough.
    Type: Grant
    Filed: June 20, 2013
    Date of Patent: January 23, 2018
    Assignee: LESAFFRE ET COMPAGNIE
    Inventors: Jean-Charles Bartolucci, Didier Colavizza, Melanie Legros, Georges Pignede
  • Patent number: 9790511
    Abstract: The present invention concerns novel Saccharomyces cerevisiae yeast strains capable of multiplying on a substrate comprising at least one C5 sugar with a speed and rate of multiplication compatible with the industrial production of yeast. It also concerns novel strains which, when cultured, make it possible to obtain yeasts having an application efficiency, i.e. an efficiency that is satisfactory in applications and uses of interest in industries such as breadmaking, biomass production, flavour production, the production of secondary metabolites, protein production, ethanol production, brewing, winemaking or the production of yeast extract.
    Type: Grant
    Filed: October 8, 2013
    Date of Patent: October 17, 2017
    Assignee: LESAFFRE ET COMPAGNIE
    Inventors: Thomas Desfougeres, Georges Pignede
  • Patent number: 9725691
    Abstract: The subject matter of the present invention is novel yeast strains capable of metabolizing xylose and resistant to at least one fermentation inhibitor, and also to the method of obtaining same. The subject of the present invention is also the yeasts obtained by culturing said yeast strains and the use thereof for producing at least one fermentation product, preferably ethanol, in particular in a culture medium comprising xylose and at least one fermentation inhibitor.
    Type: Grant
    Filed: May 24, 2013
    Date of Patent: August 8, 2017
    Assignee: LESAFFRE ET COMPAGNIE
    Inventors: Thomas Desfougeres, Georges Pignede