Abstract: Methods of making synthetic yeast cells by mating together two diploid (or higher ploidy) yeast species or hybrids to generate multi-ploid yeast hybrids are provided herein. The synthetic yeast cells made by this process and kits for performing the process are also provided.

Abstract: A method for inactivating medically important Gram-positive bacteria including Methicillin-resistant Staphylococcus aureus (MRSA), Coagulase-Negative Staphylococcus (CONS), Streptococcus, Enterococcus and Clostridium species, comprising exposure to visible light, and in particular light within the wavelength range 400-500 nm.

Abstract: A method for inactivating medically important Gram-positive bacteria including Methicillin-resistant Staphylococcus aureus (MRSA), Coagulase-Negative Staphylococcus (CONS), Streptococcus, Enterococcus and Clostridium species, comprising exposure to visible light, and in particular light within the wavelength range 400-500 nm.

Abstract: A method for inactivating medically important Gram-positive bacteria including Methicillin-resistant Staphylococcus aureus (MRSA), Coagulase-Negative Staphylococcus (CONS), Streptococcus, Enterococcus and Clostridium species, comprising exposure to visible light, and in particular light within the wavelength range 400-500 nm.

Abstract: Provided herein are constructs for genome editing or genetic engineering its fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.

Abstract: A platform apparatus for a propulsion rotor having an annular array of airfoils carried by a rotor disk. The apparatus includes: at least one forward segment comprising an arcuate forward body having a plurality of spaced-apart axially-extending forward fingers, each of the forward fingers bounded by a curved first lateral edge interconnected to a straight second lateral edge by an aft edge, wherein the forward segment defines a flow path surface and an opposed back surface; at least one aft segment comprising an arcuate aft body having a plurality of spaced-apart actually-extending aft fingers, each of the aft fingers bounded by a curved first lateral edge interconnected to a straight second lateral edge by a forward edge, wherein the aft segment defines a flow path surface and an opposed back surface, and wherein the aft edges have a shape complementary to a shape of the forward edges.

Abstract: A gas turbine including a fan platform positioned between adjacent fan blades of a fan of the gas turbine engine is provided. The fan platform defines an outer surface that at least partially defines an inner flowpath boundary of the fan. Additionally, the fan platform includes at least two components attached to one another. At least one of the components of the fan platform defines an enclosed void. An insert is positioned within the enclosed void formed of a material defining a relatively low Young's modulus. The insert may prevent resin from filling the enclosed void during assembly/manufacture of the fan platform, while also reducing a weight of the fan platform and an amount of stress on the fan platform.

Abstract: A platform apparatus for a propulsion rotor having an annular array of airfoils carried by a rotor disk. The apparatus includes: at least one forward segment comprising an arcuate forward body having a plurality of spaced-apart axially-extending forward fingers, each of the forward fingers bounded by a curved first lateral edge interconnected to a straight second lateral edge by an aft edge, wherein the forward segment defines a flow path surface and an opposed back surface; at least one aft segment comprising an arcuate aft body having a plurality of spaced-apart actually-extending aft fingers, each of the aft fingers bounded by a curved first lateral edge interconnected to a straight second lateral edge by a forward edge, wherein the aft segment defines a flow path surface and an opposed back surface, and wherein the aft edges have a shape complementary to a shape of the forward edges.

Abstract: A method for inactivating medically important Gram-positive bacteria including Methicillin-resistant Staphylococcus aureus (MRSA), Coagulase-Negative Staphylococcus (CONS), Streptococcus, Enterococcus and Clostridium species, comprising exposure to visible light, and in particular light within the wavelength range 400-500 nm.

Abstract: Provided herein are constructs for genome editing or genetic engineering its fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.

Abstract: Provided herein are constructs for genome editing or genetic engineering in fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.

Abstract: A method for inactivating medically important Gram-positive bacteria including Methicillin-resistant Staphylococcus aureus (MRSA), Coagulase-Negative Staphylococcus (CONS), Streptococcus, Enterococcus and Clostridium species, comprising exposure to visible light, and in particular light within the wavelength range 400-500 nm.

Abstract: A gas turbine including a fan platform positioned between adjacent fan blades of a fan of the gas turbine engine is provided. The fan platform defines an outer surface that at least partially defines an inner flowpath boundary of the fan. Additionally, the fan platform includes at least two components attached to one another. At least one of the components of the fan platform defines an enclosed void. An insert is positioned within the enclosed void formed of a material defining a relatively low Young's modulus. The insert may prevent resin from filling the enclosed void during assembly/manufacture of the fan platform, while also reducing a weight of the fan platform and an amount of stress on the fan platform.

Abstract: A gas turbine engine including a component having a first and a second component that are attached or integrally formed with each other. The first and the second component define an enclosed void. An insert is positioned within the enclosed void which is formed of a material having a relatively low Young's modulus. The insert may prevent resin from filling the enclosed void during assembly/manufacture of the component, while also reducing a weight of the component and an amount of stress on the component. The component may be a fan platform positioned between a plurality of fan blades, an outlet guide vane, or another component of the gas turbine engine.

Abstract: Described are a gas turbine engine fan blade platform, related rotor assembly and gas turbine engine, as well as a method of assembling the same. The platform has a forward portion proximal to an axis of rotation, an aft portion, and a transition portion between the forward and aft portions. The forward portion has a forward interface surface facing axially forward, the aft portion has an aft interface surface facing radially outward, and the transition portion has at least one mounting feature. For the method of assembly, an aft support is installed on a fan disk and booster spool assembly. A plurality of fan blades are installed into a fan disk, followed by installing a fan platform between adjacent blades and securing the mounting features to the disk, thereby filling the annulus of the fan disk. Finally, a forward support is installed on the fan disk.

Abstract: Provided herein are constructs for genome editing or genetic engineering in fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.

Abstract: A fan blade for a gas turbine engine includes: a straight axial dovetail, an airfoil, and a transition section disposed between the dovetail and the airfoil, the fan blade having opposed pressure and suction sides, and further including at least one shoulder protruding from a nominal surface of a selected one of the pressure and suction sides, wherein the at least one shoulder includes a boss defining a side face, and an upper section extending radially outward from the boss and tapering inward to join a nominal surface of the selected side.

Abstract: A method for inactivating medically important Gram-positive bacteria including Methicillin-resistant Staphylococcus aureus (MRSA), Coagulase-Negative Staphylococcus (CONS), Streptococcus, Enterococcus and Clostridium species, comprising exposure to visible light, and in particular light within the wavelength range 400-500 nm.

Abstract: A method for inactivating medically important Gram-positive bacteria including Methicillin-resistant Staphylococcus aureus (MRSA), Coagulase-Negative Staphylococcus (CONS), Streptococcus, Enterococcus and Clostridium species, comprising exposure to visible light, and in particular light within the wavelength range 400-500 nm.

Abstract: The acceleration of a mobile asset is sensed and compared to threshold acceleration levels when the speed of the mobile asset is above a threshold speed. When the acceleration exceeds a threshold a report is created allowing aggressive driving behavior to be logged.