Abstract: The substance to be tested is premixed by gentle agitation in a glass cuvette with an excess of firefly essence and with a buffer solution and cutting agent, so that any live organisms present are still alive and intact after the mixing has taken place. This gentle agitation is carried out by means of a vibratory tissue disintegrator 11 with the cuvette placed in a container 14 at one end of a reed 13 vibrated by an electromagnet 22. After this the cuvette is violently agitated by the vibratory tissue disintegrator 11 so that the cells are disrupted of any living organisms in the substance, to cause release of about one hundred ATP molecules per cell. Because of the pre-mixing, all or most of the ATP molecules react with the firefly essence almost immediately upon release from the cell, to produce a burst of light which can be distinguished from individual photons produced by stray ATP molecules and from noise within the apparatus.