Abstract: Disclosed herein are methods for regulating fungal gene expression, and reagents for carrying out those methods.

Abstract: Disclosed herein are methods for regulating fungal gene expression, and reagents for carrying out those methods.

Abstract: The present invention relates to methods of identifying a drug that inhibits the virulence of a fungus comprising contacting the fungus with a drug to be assessed and determining whether an enzyme involved in the glyoxylate pathway of the fungus is inhibited in the presence of the drug, wherein if an enzyme of the glyoxylate pathway of the fungus is inhibited in the presence of the drug, then the drug inhibits or virulence of the fungus. In particular embodiments, the present invention relates to methods of identifying a drug that inhibits the virulence of a yeast; methods of identifying a drug that inhibits the virulence of C. albicans; and methods of treating an individual who is susceptible to fungal infection comprising administering to the individual a drug identified by the methods described herein.

Abstract: Disclosed herein is a chimeric protein comprising a pre-activated transcription factor and a strong transcription activation domain for regulating fungal gene expression, and reagents and methods for constructing and using said protein.

Abstract: The invention relates to genes and proteins, namely, BON1, BON2, BON3, BAP1 and BAL, the expression of which modulate the size of a plant, and transgenic plants comprising those genes and proteins, and method of making and using same.

Abstract: The present invention relates to a transgenic plant which is tolerant to a salt, comprising one or more plant cells transformed with exogenous nucleic acid which alters expression of vacuolar pyrophosphatase in the plant. Also encompassed by the present invention are transgenic progeny and seeds of the transgenic plants described herein. Progeny transgenic plant grown from seed are also described. The present invention also relates to a construct comprising an AVP1 gene operably linked to a chimeric promoter designed to overexpress AVP1 or designed to down regulate endogenous pyrophosphatase. Plant cells (e.g., root cells, stem cell, leaf cells) comprising exogenous nucleic acid which alters expression of vacuolar pyrophosphatase in the plant cell are also the subject of the present invention. Also encompassed by the present invention are methods of making a transgenic plant described herein.

Abstract: Genes regulated by protein kinase A comprising the catalytic subunits encoded by Tpk1, Tpk2 or Tpk3 are described. Methods for altering iron uptake, trehalose breakdown, water homeostasis and respiratory growth as well as methods for altering branched chain amino acid synthesis are described. Further, methods for inhibiting virulence in an organism are described.

Abstract: A method of producing fungal, particularly yeast, biofilms, such as methods of producing fungal biofilms on surfaces (e.g., solid and semi-solid surfaces) and methods of using the resulting biofilms to identify drugs that alter (inhibit or enhance) biofilm formation and/or flo11 function and to identify genes and proteins necessary and/or sufficient for biofilm formation.

Abstract: Disclosed herein are methods for regulating fungal gene expression, and reagents for carrying out those methods.

Abstract: The invention features a method of selectively killing a first microorganism. The method includes: (i) contacting the first microorganism with a second microorganism that has a microcidal compound; and (ii) allowing the first microorganism and the second microorganism to undergo fusion, whereby the microcidal compound is delivered into and kills the microorganism that forms following the fusion.

Abstract: This invention relates to the production of useful protein products by secretion from yeast cells. In particular, it relates to the identification of supersecreting mutant yeast cells, isolation of the wild-type gene which complements the mutation, and construction of non-leaky, non-reverting supersecreting strains.

Abstract: A DNA segment contains a GAL1 promoter of Saccharomyces cerevisae linked to a gene other than the galoctokinase gene, for directing the expression of the gene within a yeast cell.A GAL1 promoter portion of Saccharomyces cerevisae is linked to a foreign DNA segment for use in expressing a desired protein. Yeast cells containing the GAL1 promoter linked to a foreign DNA segment are grown in a medium containing glucose, wherein the yeast cells metabolize the glucose and are permitted to express a polypeptide when galactose is present in the medium.

Abstract: A yeast promoter inducible by the appropriate pheromone and a method of expressing a gene of interest in substantial quantities by placing it under the control of the inducible promoter. DNA encoding a protein of interest is fused or linked to a pheromone - inducible yeast promoter, such as the FUSI or the FUS2 promoter, and the fusion is inserted onto a high copy vector; the resulting product is introduced into wild type yeast cells. Stimulation of these yeast cells by the appropriate pheromone results in induction of transcription of the yeast promoter and expression of the protein of interest in substantial quantities.

Abstract: Yeast cells that express rennin or prorennin are used in disrupted cellular form to clot milk without the need for purification of the rennin or prorennin. Cheese making is simplified and reduced in cost by the avoidance of substantial purification techniques to obtain rennin or prorennin from living cells which produce these materials.

Abstract: A DNA segment containing a GAL1 promoter linked to a gene other than the galactokinase gene for directing the expression of the gene within a yeast cell.