Abstract: Isolated truncated and mutated sensor proteins derived from flavoproteins that are 12-20 KDa or less, genetically encoded for detection and imaging of protein complexes having long fluorescent lifetimes that can be 4.0 ns or greater.

Abstract: Provided is a blood analyte collection device that includes a microneedle array configured to provide fluid communication between a cellular interstitial fluid of a subject and a collection device fluid, a device chamber containing the collection device fluid, and a sequestration material in the device chamber configured to bind to a blood analyte from the cellular interstitial fluid. Also provided are methods and kits that use the subject blood analyte collection device. The subject devices, methods and kits find use in a variety of applications, such as detecting a blood analyte, such as glucose, in a subject.

Abstract: Isolated truncated and mutated sensor proteins derived from flavoproteins that are 12-20 KDa or less, genetically encoded for detection and imaging of protein complexes having long fluorescent lifetimes that can be 4.0 ns or greater.

Abstract: Macrolide analogs and methods for identifying macrolide analogs capable of unregulated inhibition of actin filament dynamics are provided. A method according to the invention includes the steps of: (a) contacting F-actin with a candidate compound; and (b) assaying the candidate compound's ability to sever the F-actin and cap resulting F-actin (+)-ends. The candidate compound is identified as a macrolide analog where said candidate compound displays an ability to sever F-actin and, following severing, the resulting F-actin (+)-end is capped by the candidate compound thusly preventing subsequent G-actin incorporation. Severing and capping activities are unregulated; i.e., independent of at least physiologically-meaningful concentrations of phosphatidyl inositol and calcium.

Abstract: The present invention provides photochromic compounds and derivatives thereof as shown in claim 1 and methods of use of these compounds and derivatives. The present invention also provides photochromic optical probes capable of undergoing light directed reversible transition between a first state and a second state. The invention also teaches methods of determining and controlling reversible optical biomolecular interactions, for example binding of calcium in a subject.

Abstract: Macrolide analogs and methods for identifying macrolide analogs capable of unregulated inhibition of actin filament dynamics are provided. A method according to the invention includes the steps of: (a) contacting F-actin with a candidate compound; and (b) assaying the candidate compound's ability to sever the F-actin and cap resulting F-actin (+)-ends. The candidate compound is identified as a macrolide analog where said candidate compound displays an ability to sever F-actin and, following severing, the resulting F-actin (+)-end is capped by the candidate compound thusly preventing subsequent G-actin incorporation. Severing and capping activities are unregulated; i.e., independent of at least physiologically-meaningful concentrations of phosphatidyl inositol and calcium.