Abstract: Disclosed is a method of artificial intelligence-based robotic pipetting for cell preparation. The method includes training an artificial intelligence/machine learning system (AI/ML system) to classify images of biological specimens. The method includes storing the classified images. The method includes receiving an image object from an imaging system that includes a microscopy system, a camera system configured to receive imaging from the microscopy system, and a lighting system configured to illuminate a biological material. The method includes processing the image object, using the AJ/ML system to determine a presence of a retrievable target cell. The method includes positioning a robotic pipettor at a target physical orientation relative to the retrievable target cell. The method includes confirming the robotic pipettor's location at the target physical orientation, using AI/ML system.

Abstract: A method of artificial-intelligence-based robotic vitrification includes placing at least one oocyte in a buffer or CPA solution. The method includes processing an image object produced by an imaging system, using an AI/ML system, to determine a location of a retrievable target oocyte. The method includes positioning a robotic pipettor at a target physical orientation relative to the retrievable target oocyte. The method includes confirming the robotic pipettor's location at the target physical orientation, using the AI/ML system. The method includes instructing the robotic pipettor to initiate contact with the retrievable target oocyte and apply negative pressure to secure the oocyte to the robotic pipettor. The method includes using a robotic microtool holder to lower a vitrification assembly or cryo-device of any other make into a buffer or CPA solution dish to a position in proximity to the target physical orientation.

Abstract: The yarns of the present invention satisfy the need for a multi-purpose yarn, capable of improved abrasion resistance, seam slippage and dimensional stability while eliminating traditional fabric's backing materials and costs associated with the production thereof. In one aspect, the method of producing yarn and fabrics comprises supplying a first yarn, supplying a low melt yarn, placing the two yarns adjacent to one another, and winding the two adjacent yarns. In another aspect, the method of producing yarns and fabrics further comprises weaving the yarns into a fabric and heating the fabric, wherein heating the fabric heats the low melt yarn, melting at least one filament and adhering the low melt yarn to the first yarn or fabric.