Abstract: The invention is directed to novel CDRs and immunoglobulin products that bind to A33 antigens and methods for their use. The invention also involves a method for making humanized antibodies, using a rabbit as a host animal, and phage display library methodologies, and the antibodies themselves. The methodology is useful, for example, in generating humanized antibodies against molecules associated with cancer, such as A33, which is associated with colon cancer.

Abstract: This invention relates to isolated proteins and to peptides which are found on the surface of colon cells and colon cancer cells, as well as to nucleic acid molecules encoding said protein and peptides. The protein and peptides bind to tumor associated antibodies, such as mAb 33. The monomeric protein has a molecular weight of about 43 kD as determined by SDS gel electrophoresis under non-reducing conditions. In addition, this invention relates to the use of said nucleic acid molecules, protein, in monomeric or multimeric form, and to antibodies to said peptides in diagnostic, screening and therapeutic methods. This invention further relates to antibodies specific for said protein, in monomeric or multimeric form, and to antibodies to said peptides.

Abstract: The invention involves the therapeutic use of humanized antibodies, fragments, etc., which bind to the antigen A33. These antibody molecules are useful in the treatment of various neoplasias, including colon cancer. Various modes of administration, and doses are described.

Abstract: The invention relates to the production of ganglioside specific antibodies. These antibodies are produced following immunization with lipopolysaccharide antigen of Campylobacter jejuni. The antibodies bind to monosialogangliosides, including GM2 and GM1.

Abstract: The invention describes an improved method for making monosialoganglioside GM3 and its intermediates. Following reaction of a neuraminic acid donor and a lactose acceptor in the presence of an acid catalyst, the .alpha. and .beta. isomers of GM3 are formed. The .alpha. isomer is converted to a lactone, via action of a ring forming basic catalyst, which is then separable from the .beta. isomer. The lactone is then treated with a basic catalyst in the presence of an alcohol, to form GM3 or a GM3 intermediate.

Abstract: The invention relates to the production of ganglioside specific antibodies. These antibodies are produced following immunization with lipopolysaccharide antigen of Campylobacter jejuni. The antibodies bind to monosialogangliosides, including GM2 and GM1.

Abstract: This invention relates to methods of reducing the effects of colon cancer tumors. Various agents are conjugated to monoclonal antibodies which are specific for colon cancer cells. The conjugates are administered to patients having colon cancer such that the effects of the cancer are reduced.

Abstract: This invention relates to methods of producing synthetic GM2s. The methods are simple and efficient, and result in high yields of GM2s.

Abstract: This invention relates to isolated protein and to peptides which are found on the surface of colon cells and colon cancer cells, as well as to nucleic acid molecules encoding said protein and peptides. The protein and peptides bind to tumor-associated antibodies, such as mAb A33. The monomeric protein has a molecular weight of about 43 kD as determined by SDS gel electrophoresis under non-reducing conditions. In addition, this invention relates to the use of said nucleic acid molecules, protein, in monomeric or multimeric form, and to antibodies to said peptides in diagnostic, screening and therapeutic methods. This invention further relates to antibodies specific for said protein, in monomeric or multimeric form, and to antibodies to said peptides.

Abstract: A filter material forming a filter layer consisting substantially exclusively of celluloses and cellulose derivatives. In addition to cellulose acetate fibers, the filter layer contains, in the form of additives, microcrystalline cellulose and microfine cellulose, or else microcrystalline cellulose only. The amount of microcrystalline cellulose can be as high as 70% of the weight of the filter layer and that of microfine cellulose up to 50% of the weight of the filter layer. Cellulose acetate fibers can be excluded if in their place, microcrystalline cellulose having particle sizes less than 10 .mu.m. are used. The manufacturing method provides for cellulose acetate fibers to be first mixed in a wet state with microfine cellulose and microcrystalline cellulose, after which differently prepared celluloses are admixed. The mash formed by mixing is formed into a fleece by means of a known felting method, and the fleece is subsequently dried.

Abstract: A flexible fleece-like filter material is provided wherein in a matrix-like basic framework consisting of synthetic or natural fibres, ultra-finely fibrillated fibres and/or inert porous particles are incorporated as a filter medium, the filter medium forming, anchored in the matrix, chain structures provided with a cationic or anionic charge or possibly charged with other substances which influence the filtering properties or with immobilised cells and/or catalysts. A fleece is formed with a thickness of between 0.5 mm and 5 mm and preferably up to 2 mm, which is flexible and which can even be pleated. This filter material can be produced by a simple method without harming the environment.

Abstract: In a filter bed (11) for the solid/fluid separation of media which contain substances that attack or dissolve cellulose, for example cellulase-containing enzymes, a matrix comprising a fibrous material structure of polyolefin fibrid particles (12) which are matted and anchored in one another and which can additionally contain up to 20% by weight of narrower fibrid polyolefin fibres and/or polyolefin fibres that have a fibre length of up to 5 mm. and have just started to fibrillate, in order to strengthen the sheet formation of the matrix. The actual filter-active particles, in particular kieselguhr particles and/or perlite particles having grain sizes of 5 to 100 um., are embedded in the cellulose-free fiber matrix.