Abstract: The subject relates to an isolated antibody that specifically binds to O25b antigen of multi drug resistant (MDR) E. coli strains, its medical and diagnostic use, method of producing the antibody, including an isolated nucleotide sequence, plasmids and host cells as used in the production of the antibody; and further an isolated epitope recognized the specific antibody.

Abstract: The subject relates to an isolated antibody that specifically binds to O25b antigen of multi drug resistant (MDR) E. coli strains, its medical and diagnostic use, method of producing the antibody, including an isolated nucleotide sequence, plasmids and host cells as used in the production of the antibody; and further an isolated epitope recognized the specific antibody.

Abstract: The subject relates to an isolated antibody that specifically binds to O25b antigen of multi drug resistant (MDR) E. coli strains, its medical and diagnostic use, method of producing the antibody, including an isolated nucleotide sequence, plasmids and host cells as used in the production of the antibody; and further an isolated epitope recognized the specific antibody.

Abstract: The subject relates to an isolated antibody that specifically binds to O25b antigen of multi drug resistant (MDR) E. coli strains, its medical and diagnostic use, method of producing the antibody, including an isolated nucleotide sequence, plasmids and host cells as used in the production of the antibody; and further an isolated epitope recognized the specific antibody.

Abstract: The subject relates to an isolated antibody that specifically binds to O25b antigen of multi drug resistant (MDR) E. coli strains, its medical and diagnostic use, method of producing the antibody, including an isolated nucleotide sequence, plasmids and host cells as used in the production of the antibody; and further an isolated epitope recognized the specific antibody.

Abstract: The subject relates to an isolated antibody that specifically binds to O25b antigen of multi drug resistant (MDR) E. coli strains, its medical and diagnostic use, method of producing the antibody, including an isolated nucleotide sequence, plasmids and host cells as used in the production of the antibody; and further an isolated epitope recognized the specific antibody.

Abstract: The subject relates to an isolated antibody that specifically binds to O25b antigen of multi drug resistant (MDR) E. coli strains, its medical and diagnostic use, method of producing the antibody, including an isolated nucleotide sequence, plasmids and host cells as used in the production of the antibody; and further an isolated epitope recognized the specific antibody.

Abstract: The subject relates to an isolated antibody that specifically binds to O25b antigen of multi drug resistant (MDR) E. coli strains, its medical and diagnostic use, method of producing the antibody, including an isolated nucleotide sequence, plasmids and host cells as used in the production of the antibody; and further an isolated epitope recognized the specific antibody.

Abstract: The subject relates to an isolated antibody that specifically binds to O25b antigen of multi drug resistant (MDR) E. coli strains, its medical and diagnostic use, method of producing the antibody, including an isolated nucleotide sequence, plasmids and host cells as used in the production of the antibody; and further an isolated epitope recognized the specific antibody.

Abstract: A method of detection of an analyte in which (i) a protein comprising a moiety capable of binding the analyte and a fluorescent label is contacted with a medium suspected of containing the analyte; (ii) the analyte, if present, binds to the moiety; (iii) the protein is subjected to incident radiation to excite the protein and induce intrinsic emission therefrom; whereby the intrinsic emission from the protein is converted through Fluorescence Resonance Energy Transfer (FRET) into emission from the fluorescent label and the amount of said FRET is affected by the binding of the analyte to the moiety; and, (iv) the emission from the fluorescent label is measured; whereby the level of emission from the fluorescent label is indicative of the presence of the analyte, and wherein the protein undergoes no substantial conformational charge during the method. A kit for carrying out the method of the invention and a protein are also provided.

Abstract: The present invention concerns a method of detection of an analyte in which a protein capable of binding the analyte and comprising a fluorescent energy label and an energy acceptor moiety capable of accepting energy emitted by the label or protein by Forster energy transfer (FRET), is exposed to incident electromagnetic energy to excite the protein or label, and the fluorescent emission of the label is measured; characterised in that the protein is encapsulated in a biocompatible, optically transparent matrix which is permeable to the analyte, and in that the protein undergoes no substantial conformational change during the method; further characterised in that the energy acceptor moiety has a more active and less active state, which is determined by the presence of analyte, and the emission from the label is indicative of the presence of analyte. A biocompatible optically transparent matrix in which a protein capable of binding an analyte is also provided.