Abstract: Methods and devices for generating ionized molecules for analysis in a mass spectrometer are provided. A device comprises a solid substrate having one or more edges for spray ionization, the substrate adapted for receiving extraction phase comprising molecules of interest. The solid substrate may comprise one or more indentations for receiving the extraction phase and desorption solvent. The indentation may extend to one of the edges of the substrate to channel the desorption solution to the edge for spray ionization. The solid substrate may comprise a magnetic portion for retaining magnetic extraction phase deposited thereon. The solid substrate itself may be free of any extraction phase prior to an extraction phase containing the molecules of interest being deposited thereon.

Abstract: Methods and systems for delivering a liquid sample to an ion source for the generation of ions and subsequent analysis by mass spectrometry are provided herein. In accordance with various aspects of the present teachings, MS-based systems and methods are provided in which a desorption solvent utilized in a sampling interface to desorb one or more analyte species from an SPME device is fluidly coupled to an ion source for ionizing the one or more analyte species desorbed into the desorption solvent for subsequent mass spectrometric analysis (e.g., without a liquid chromatography (LC) column between the sampling interface and the ion source).

Abstract: The present disclosure describes a device for generating ionized molecules for analysis in a mass spectrometer. The device includes: a mesh substrate coated with an extraction phase, the extraction phase comprising a polymer that absorbs a molecule of interest from a matrix, or a polymer and solid phase microextraction (SPME) particles having pores dimensioned to absorb a molecule of interest from a matrix, where the mesh substrate has a sufficiently open structure to allow fluid to flow through the mesh substrate; and a solid substrate connected to the mesh substrate to provide stability to the coated mesh substrate. Mass spectrometry systems that include such a device are also described. Methods of analyzing an analyte previously extracted from a matrix onto the device are also described.

Abstract: Methods and systems for delivering a liquid sample to an ion source for the generation of ions and subsequent analysis by mass spectrometry are provided herein. In accordance with various aspects of the present teachings, MS-based systems and methods are provided in which a desorption solvent utilized in a sampling interface to desorb one or more analyte species from an SPME device is fluidly coupled to an ion source for ionizing the one or more analyte species desorbed into the desorption solvent for subsequent mass spectrometric analysis (e.g., without a liquid chromatography (LC) column between the sampling interface and the ion source).

Abstract: A device is described for generating ionized molecules for analysis in a mass spectrometer. The device includes: a solid substrate having one or more edges and a coated area that is coated with an extraction phase comprising an extraction polymer. The solid substrate may have at least two edges that meet at an angle from about 8° to about 180°. Mass spectrometry systems that include such a device are also described. Methods of analyzing a molecule previously extracted from a sample onto the device are also described.

Abstract: The invention describes the process by which a standard mixture of organic compounds are spiked and retained onto a composite sorbent matrix for the controlled generation of a standard in fluid above the spiked matrix either in a gaseous or aqueous phase. The novelty of the aforementioned composite matrix stems from the combination of an immobilizing liquid phase such as silicone oil or a polyacrylonitrile solution, and solid, porous particles such as polystyrene-co-divinylbenzene (PS-DVB) or hydrophilic/Lipophilic Balance (HLB) particles to strongly retain the spiked standards. These novel composite mixtures exhibit sorptive capabilities greater than the sum of their individual components. In addition swelling of the particles with the liquid phase facilitates immobilization of the composite sorbent matrix in the vial.

Abstract: The invention describes the process by which a standard mixture of organic compounds are spiked and retained onto a composite sorbent matrix for the controlled generation of a standard in fluid above the spiked matrix either in gas (headspace) or aqueous phase which is contained in portable vials. The novelty of the aforementioned composite matrix stems from the combination of an immobilizing liquid phase such as silicone oil or a polyacrylonitrile solution, and solid, porous particles such as polystyrene-co-divinylbenzene (PS-DVB) or Hydrophilic/Lipophilic Balance (HLB) particles to strongly retain the spiked standards facilitating high capacity which translates into prolonged use as a source of calibrant. These novel composite mixtures exhibit sorptive capabilities greater than the sum of their individual components producing a standard analyte generator far superior to those described in the prior art.

Abstract: A device is described for generating ionized molecules for analysis in a mass spectrometer. The device includes: a solid substrate having one or more edges and a coated area that is coated with an extraction phase comprising an extraction polymer. The solid substrate may have at least two edges that meet at an angle from about 8° to about 180°. Mass spectrometry systems that include such a device are also described. Methods of analyzing a molecule previously extracted from a sample onto the device are also described.

Abstract: The present disclosure describes a device for generating ionized molecules for analysis in a mass spectrometer. The device includes: a mesh substrate coated with an extraction phase, the extraction phase comprising a polymer that absorbs a molecule of interest from a matrix, or a polymer and solid phase microextraction (SPME) particles having pores dimensioned to absorb a molecule of interest from a matrix, where the mesh substrate has a sufficiently open structure to allow fluid to flow through the mesh substrate; and a solid substrate connected to the mesh substrate to provide stability to the coated mesh substrate. Mass spectrometry systems that include such a device are also described. Methods of analyzing an analyte previously extracted from a matrix onto the device are also described.