Abstract: The invention relates to a method for preparing a comestible nutrient composition comprising the steps of (i) cultivating proliferating non-human animal cells of interest in vitro until the cell count of said cells has multiplied by at least 2-fold or more; (ii) harvesting the cells to provide cell-derived material; and (iii) preserving the cell-derived material from the cells harvested in step (ii) from spoiling. Furthermore, the invention refers to a comestible nutrient composition obtainable from such method and use thereof for providing a well-defined nutrient composition.

Abstract: The invention provides a method of identifying whether a protein target and a compound of interest bind to one another, by exposing the protein target to the compound of interest for a time sufficient to allow binding of the compound of interest to the protein target, treating with a chemical denaturant and assaying the products of the denaturing mixture to determine the proportion of the protein target in its folded state and/or the proportion of the protein target in its denatured state. Suitably, the method is conducted at a substantially constant temperature. A change in the proportion of the protein target in its folded state as compared to a suitable control indicates that the protein target and compound of interest bind to one another.