Abstract: Described is a method of producing an oligosaccharide comprising a lacto-N-triose (LN3; GlcNAc-beta1,3-Gal-beta1,4-Glc) as a core trisaccharide by cultivation with a genetically modified cell, as well as the genetically modified cell used in the method. The genetically modified cell comprises at least one nucleic acid sequence coding for a galactoside beta-1,3-N-acetylglucosaminyltransferase and a glycosyltransferase involved in the synthesis of an oligosaccharide comprising LN3 as a core trisaccharide and at least one nucleic acid sequence expressing a membrane protein. Furthermore, the present invention provides for a purification of the oligosaccharide comprising LN3 as a core trisaccharide from the cultivation.

Abstract: Described is a method of producing bioproducts by fermentation with a genetically modified cell, as well as to the genetically modified cell used in the method. The cell is genetically modified to produce a bioproduct and is further genetically modified by reducing the expression of at least one endogenous membrane protein encoding gene and/or mutating the expression of the endogenous membrane protein.

Abstract: The disclosure is in the technical field of synthetic biology and metabolic engineering. Described is the use of a new type of galactosyltransferases for the production of a galactosylated di- or oligosaccharide. The disclosure also describes methods for the production of a galactosylated di- or oligosaccharide as well as the purification of the di- or oligosaccharide. Furthermore, the disclosure is in the field of cultivation or fermentation of metabolically engineered cells. The disclosure provides a cell metabolically engineered for production of a galactosylated di- or oligosaccharide.

Abstract: This disclosure is in the technical field of synthetic biology and metabolic engineering. More particularly, this disclosure is in the technical field of fermentation of metabolically engineered microorganisms. This disclosure describes engineered micro-organisms able to synthesize sialylated compounds via an intracellular biosynthesis route. These micro-organisms can dephosphorylate N-acetylglucosamine-6-phosphate to N-acetyl glucosamine and convert the N-acetylglucosamine to N-acetylmannosamine. These micro-organisms also have the ability to convert N-acetylmannosamine to N-acetyl-neuraminate.

Abstract: The disclosure is in the technical field of synthetic biology and metabolic engineering. More particularly, the disclosure is in the technical field of metabolically engineered cells and use of the cells in a cultivation or fermentation. The disclosure describes a metabolically engineered cell and a method by cultivation or fermentation with the cell for production of a sialylated di- and/or oligosaccharide. The metabolically engineered cell comprises a pathway for production of the sialylated di- and/or oligosaccharide and is modified for expression and/or overexpression of multiple coding DNA sequences encoding one or more isoproteins that catalyze the same chemical reaction. Furthermore, the disclosure provides for purification of the sialylated di- and/or oligosaccharide from the cultivation.

Abstract: The disclosure is in the technical field of synthetic biology and metabolic engineering. More particularly, the disclosure is in the technical field of cultivation or fermentation of metabolically engineered cells. The disclosure describes a method for the production of a di- or oligosaccharide with an N-acetylglucosamine at the reducing end by a cell as well as the purification of the di- or oligosaccharide from the cultivation. Furthermore, the disclosure provides a cell metabolically engineered for production of a di- or oligosaccharide with an N-acetylglucosamine at the reducing end.

Abstract: This disclosure is in the technical field of synthetic biology and metabolic engineering. More particularly, this disclosure is in the technical field of cultivation or fermentation of metabolically engineered cells. This disclosure provides a method for the production of a mixture of at least two different oligosaccharides by a cell as well as the purification of at least one of the oligosaccharides from the cultivation. In addition, this disclosure provides a method for the production of a mixture of at least two different oligosaccharides by a metabolically engineered cell as well as the purification of at least one of the oligosaccharides from the cultivation.

Abstract: The disclosure is in the technical field of synthetic biology and metabolic engineering. More particularly, the disclosure is in the technical field of cultivation or fermentation of metabolically engineered cells. The disclosure describes a cell metabolically engineered for production of an alpha-1,3 glycosylated form of fucose-alpha1,2-galactose-R (Fuc-a1,2-Gal-R). Furthermore, the disclosure provides a method for the production of an alpha-1,3 glycosylated form of Fuc-a1,2-Gal-R by a cell as well as the purification of the alpha-1,3 glycosylated form Fuc-a1,2-Gal-R from the cultivation.

Abstract: The disclosure is in the technical field of synthetic biology and metabolic engineering. More particularly, the disclosure is in the technical field of cultivation or fermentation of metabolically engineered cells. The disclosure describes a cell metabolically engineered for production of a mixture of at least four different neutral non-fucosylated oligosaccharides. Furthermore, the disclosure provides a method for the production of a mixture of at least four different neutral non-fucosylated oligosaccharides by a cell as well as the purification of at least one of the oligosaccharides from the cultivation.

Abstract: The present invention is in the technical field of synthetic biology and metabolic engineering. More particularly, the present invention is in the technical field of fermentation of metabolically engineered microorganisms. The present invention describes engineered microorganisms able to synthesize sialylated compounds via an intracellular biosynthesis route. These microorganisms can dephosphorylate N-acetylglucosamine-6-phosphate to N-acetylglucosamine and convert the N-acetylglucosamine to N-acetylmannosamine. These microorganisms also have the ability to convert N-acetylmannosamine to N-acetyl-neuraminate.

Abstract: The present disclosure is in the technical field of synthetic biology and metabolic engineering. The disclosure provides engineered viable bacteria. In particular, the disclosure provides viable bacteria with reduced cell wall biosynthesis additionally modified for production of glycosylated product. The disclosure further provides methods of generating viable bacteria and uses thereof. Furthermore, the disclosure in the technical field of fermentation of metabolically engineered microorganisms producing glycosylated product.

Abstract: The present invention is in the technical field of synthetic biology and metabolic engineering. More particularly, the present invention relates to a method to determine the expression stability of a heterologous gene at a chromosomal location in a cell undergoing burden and to produce mutated cells or organisms transformed with a heterologous gene at a chromosomal location, wherein the expression of said heterologous gene is not influenced by a burden or wherein the expression of said heterologous gene is reduced by a burden. The present invention describes methods to locate interesting chromosomal knock-in locations in a cell. Such engineered cells and organisms are applied for the production of bioproducts, such as but not limited to carbohydrates, lipids, proteins, organic acids, amino acids, alcohols, antibiotics and peptides. Preferably, the invention is applied in the technical field of fermentation of metabolically engineered microorganisms.

Abstract: A running shoe sole has an elastically deformable supporting sole, which has a rear and front foot section connected to each other via a coupling section. An elastically deformable supporting device is arranged on the supporting sole and carries an outsole covering. The supporting device includes a rear foot part which engages around the rear foot section of the supporting sole in a U shape and a front foot part having two limbs which are arranged on mutually opposite lateral edge sections of the front foot section. The rear and front foot part each have a supporting surface, which runs obliquely inward toward the underside of the shoe sole or is curved, preferably convex, and on which the supporting sole rests and is supported in the lateral direction. The rear and front foot parts are made from a highly responsive thermoplastic elastomer of 45-50 Asker Shore C density.

Abstract: The present invention is in the technical field of synthetic biology and metabolic engineering. More particularly, the present invention is in the technical field of fermentation of metabolically engineered microorganisms. The present invention describes engineered microorganisms able to synthesize sialylated compounds via an intracellular biosynthesis route. These microorganisms can dephosphorylate N-acetylglucosamine-6-phosphate to N-acetylglucosamine and convert the N-acetylglucosamine to N-acetylmannosamine. These microorganisms also have the ability to convert N-acetylmannosamine to N-acetyl-neuraminate.

Abstract: A method for controlling the robot of a training system according to any of the previous claims, wherein a biomechanical and/or cardiovascular stress of the user, particularly based on a measured impingement of the actuation surface, is determined and the robot is controlled using a predetermined and the measured biomechanical and/or cardiovascular stress of the user. A computer program product with a program code, which is saved on a medium readable by the computer, for implementing a method according to the previous claim.

Abstract: An orthosis for stabilizing a joint has a joint rail having a first and a second rail portion which can be pivoted relative to each other about a joint axle. The orthosis has a pressure member which can be adjusted by a relative pivoting of the two rail portions about the joint axle in the direction towards and counter to the joint to be stabilized. One of the two rail portions has a cam disc, and the other of the two rail portions has a rotor which is guided on the cam disc, wherein the cam disc or the rotor is axially fixed in position on the correction pad.

Abstract: An orthopedic brace system for bracing a joint with a dimensionally stable, flexible base body, which can be placed around the members adjoining the joint and has closure devices by way of which the base body can be fixed to the members adjoining the joint. A dimensionally stable outer frame extending over the joint is removably attached to the base body by way of adjustable fastening members, wherein the outer frame is braced on the members adjoining the joint.

Abstract: An orthesis for stabilizing a joint has a joint rail having a first and a second rail portion which can be pivoted relative to each other about a joint axle. The orthesis has a pressure member which can be adjusted by a relative pivoting of the two rail portions about the joint axle in the direction towards and counter to the joint to be stabilized. One of the two rail portions has a cam disc, and the other of the two rail portions has a rotor which is guided on the cam disc, wherein the cam disc or the rotor is axially fixed in position on the correction pad.

Abstract: A lower-leg orthosis includes a splint for immobilizing the muscles of the lower leg. The splint comprises a lower-leg cuff and of a foot rest connected to the lower-leg cuff. A foot device is arranged under the foot rest, and the foot rest is supported on the ground via this foot device. The foot device has a ground contact surface and an elastic energy reservoir, which is arranged between the foot rest and the ground contact surface. Torques can be transferred from the foot rest surface to the ground contact surface via the foot device.

Abstract: In a knee-joint orthosis for guiding the patella of a patient during the transition from an extended position to a flexed position of the knee joint and vice versa, with a fastening means (I) for fastening to a thigh and a lower leg, with at least one laterally arranged stabilizing element that takes up a flexion of the knee joint and bears on the thigh and lower leg, and with a dimensionally stable guide element (6) that can be held in position by an arrangement of tensioning straps and is arranged to bear laterally on the patella by way of a lateral base part (7) from which medially directed attachments (8) extend proximally and distally of the patella and are secured to strap sections (10, 12) on the orthosis in the area of the thigh and of the lower leg in such a way that a medial tensile force is exerted on the attachments (8).