Patents by Inventor Gia-Luen Guo
Gia-Luen Guo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20180179568Abstract: Lignocellulose is pretreated using solvent with little water footprint. Both a solvent and water are used for saccharification and separating the components of lignocellulose. By adding a small amount of an inorganic acid, lignin is dissolved and hemicellulose is hydrolyzed under appropriate reaction temperature and pressure. Both are dissolved to form liquid phase with solid cellulose residue left. The relative volatility difference between the solvent and water is used to selectively remove more of the solvent. More particularly, drying with air-suction at a relatively low temperature helps remove and recycle the solvent from the cellulose solid residue. Thus, water consumption and the subsequent wastewater treatment can be significantly reduced with little water footprint while achieving the original desired efficiency of enzymatic hydrolysis.Type: ApplicationFiled: December 27, 2016Publication date: June 28, 2018Inventors: Fong-Yu Yen, Hsin-Hung Chen, Gia-Luen Guo, Ming-Feng Jang, Wen-Hua Chen
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Patent number: 9297001Abstract: A preparation method conducive to enhancing enzymatic activity of cellulase includes the steps of preparing a first inducer, preparing preculture hyphae, producing enzymes by a single strain, feeding a second inducer, and producing enzymes by co-culture strains. Main ingredients of the first inducer and the second inducer are cellulose and lactose, respectively. The enzymatic activity of the cellulase produced is enhanced by induction of cellulose and lactose and co-culture of Trichoderma and Aspergillus.Type: GrantFiled: August 28, 2014Date of Patent: March 29, 2016Assignee: INSTITUTE OF NUCLEAR ENERGY RESEARCH, ATOMIC ENERGY COUNCIL, EXECUTIVE YUANInventors: Chun-An Wang, Sheng-Hsin Chou, Gia-Luen Guo
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Publication number: 20150118717Abstract: A preparation method conducive to enhancing enzymatic activity of cellulase includes the steps of preparing a first inducer, preparing preculture hyphae, producing enzymes by a single strain, feeding a second inducer, and producing enzymes by co-culture strains. Main ingredients of the first inducer and the second inducer are cellulose and lactose, respectively. The enzymatic activity of the cellulase produced is enhanced by induction of cellulose and lactose and co-culture of Trichoderma and Aspergillus.Type: ApplicationFiled: August 28, 2014Publication date: April 30, 2015Inventors: CHUN-AN WANG, SHENG-HSIN CHOU, GIA-LUEN GUO
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Patent number: 8921082Abstract: A method for producing xylitol by fermentation of lignocellulosic hydrolysates without detoxification is provided. By using the originally isolated yeast Candida sp., xylose can be effectively converted into xylitol. The invention also provides the Candida strain having high furfural tolerance, and is capable to produce xylitol from various types of non-detoxified lignocellulosic hydrolysates, in which the overall utilization of xylose in hydrolysate can reach over 95%.Type: GrantFiled: March 1, 2013Date of Patent: December 30, 2014Assignee: Institute of Nuclear Energy Research Atomic Energy Council, Executive YuanInventors: Chiung-Fang Huang, Yi-Feng Jiang, Ting-Hsiang Lin, Gia-Luen Guo, Wen-Song Hwang, Jia-Baau Wang
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Patent number: 8518687Abstract: A cellulose hydrolase and a gene thereof are obtained by screening a cDNA genomic library constructed with Orpinomyces sp. Y102. The gene is 1071 base pairs long and comprises an open reading frame (ORF) for producing the cellulose hydrolase comprising 357 amino acids by translation. A transformed cell and a carrier carrying the gene are introduced. The gene is transferred to E. coli by transformation, such that E. coli can acquire activity of decomposing CMC, beta-glucan, and xylan. The cellulose hydrolase is multifunctional and is capable of decomposing cellubiose and directly decomposing fiber into glucose.Type: GrantFiled: March 20, 2012Date of Patent: August 27, 2013Assignees: National Pingtung University of Science and Technology, Institute of Nuclear Energy Research, Atomic Energy Council, Executive YuanInventors: Yo-Chia Chen, Jeng-Chen Liu, Hau-Yu Wang, Teng-Chieh Hsu, Gia-Luen Guo, Wen-Song Hwang, Jia-Baau Wang
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Publication number: 20130102051Abstract: A cellulose hydrolase and a gene thereof are obtained by screening a cDNA genomic library constructed with Orpinomyces sp. Y102. The gene is 1071 base pairs long and comprises an open reading frame (ORF) for producing the cellulose hydrolase comprising 357 amino acids by translation. A transformed cell and a carrier carrying the gene are introduced. The gene is transferred to E. coli by transformation, such that E. coli can acquire activity of decomposing CMC, beta-glucan, and xylan. The cellulose hydrolase is multifunctional and is capable of decomposing cellubiose and directly decomposing fiber into glucose.Type: ApplicationFiled: March 20, 2012Publication date: April 25, 2013Applicants: National Pingtung University of Science and Technology, Institute of Nuclear Energy Research, Atomic Energy Council, Executive YuanInventors: Yo-Chia Chen, Jeng-Chen Liu, Hau-Yu Wang, Teng-Chieh Hsu, Gia-Luen Guo, Wen-Song Hwang, Jia-Baau Wang
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Patent number: 8409835Abstract: A method for producing xylitol by fermentation of lignocellulosic hydrolysates without detoxification is provided. By using the originally isolated yeast Candida sp., xylose can be effectively converted into xylitol. The invention also provides the Candida strain having high furfural tolerance, and is capable to produce xylitol from various types of non-detoxified lignocellulosic hydrolysates, in which the overall utilization of xylose in hydrolysate can reach over 95%.Type: GrantFiled: May 7, 2010Date of Patent: April 2, 2013Assignee: Institute of Nuclear Energy Research Atomic Energy Council, Executive YuanInventors: Chiung-Fang Huang, Yi-Feng Jiang, Ting-Hsiang Lin, Gia-Luen Guo, Wen-Song Hwang, Jia-Baau Wang
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Patent number: 8278078Abstract: A method for culturing the yeast for enhancing pentitol production is provided. The yeast cultured according to the present disclosure is Pichia stipitis. Application of the yeast in pentitol production by fermention of the lignocellulosic hydrolysate or the xylose-to-pentitol production yield can be enhanced 3 to 6 times from the non-detoxified or the overliming-processed lignocellulosic hydrolysate.Type: GrantFiled: May 28, 2010Date of Patent: October 2, 2012Assignee: Atomic Energy Council-Institute of Nuclear Energy ResearchInventors: Chiung-Fang Huang, Wei-Hsi Chen, Ting-Hsiang Lin, Wen-Heng Chen, Gia-Luen Guo, Wen-Song Hwang
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Publication number: 20110177572Abstract: A hydrolysate-adapted yeast, Pichia stipitis INER 1128, is cultivated according to the present invention. The adapted yeast can effectively convert xylose into ethanol in lignocellulosic hydrolysate, which is not even detoxified. Well ethanol yield is obtained while xylose is not wasted and thus cost is reduced.Type: ApplicationFiled: January 19, 2010Publication date: July 21, 2011Applicant: ATOMIC ENERGY COUNCIL-INSTITUTE OF NUCLEAR ENERGY RESEARCHInventors: Jia-Baau Wang, Lee-Chung Men, Wen-Song Hwang, Wen-Hua Chen, Sheng-Shieh Chen, Wen-Heng Chen, Ting-Hsiang Lin, Chiung-Fang Huang, Sheng-Hsin Chou, Teng-Chieh Hsu, Gia-Luen Guo
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Publication number: 20110104775Abstract: The present disclosure is related to a method for increasing the ethanol concentration from the conversion of lignocellulose. The pretreated solid residues are mixed with ethanol-containing broth from the fermentation of xylose hydrolysate by Pichia stipitis and then are performed under the process of simultaneous saccharification and fermentation (SSF) with Sacharomyces cerevisiae and cellulase for converting cellulose to ethanol. The final ethanol concentration in broth as well as the ethanol productivity is increased at least 1.8 times in comparison of conventional process for lignocellulosic ethanol production.Type: ApplicationFiled: August 13, 2010Publication date: May 5, 2011Applicant: ATOMIC ENERGY COUNCIL-INSTITUTE OF NUCLEAR ENERGY RESEARCHInventors: Teng-Chieh Hsu, Ting-Hsiang Lin, Tien-Yang Ma, Gia-Luen Guo, Wen-Song Hwang, Jia-Baau Wang
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Publication number: 20110104772Abstract: A method for culturing the yeast for enhancing pentitol production is provided. The yeast cultured according to the present disclosure is Pichia stipitis.Type: ApplicationFiled: May 28, 2010Publication date: May 5, 2011Applicant: ATOMIC ENERGY COUNCIL-INSTITUTE OF NUCLEAR ENERGY RESEARCHInventors: Chiung-Fang Huang, Wei-Hsi Chen, Ting-Hsiang Lin, Wen-Heng Chen, Gia-Luen Guo, Wen-Song Hwang
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Publication number: 20110097772Abstract: A method for producing xylitol by fermentation of lignocellulosic hydrolysates without detoxification is provided. By using the originally isolated yeast Candida sp., xylose can be effectively converted into xylitol. The invention also provides the Candida strain having high furfural tolerance, and is capable to produce xylitol from various types of non-detoxified lignocellulosic hydrolysates, in which the overall utilization of xylose in hydrolysate can reach over 95%.Type: ApplicationFiled: May 7, 2010Publication date: April 28, 2011Applicant: Institute of Nuclear Energy Research Atomic Energy Council, Executive YuanInventors: Chiung-Fang Huang, Yi-Feng Jiang, Ting-Hsiang Lin, Gia-Luen Guo, Wen-Song Hwang, Jia-Baau Wang
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Publication number: 20110097780Abstract: A method is provided for improving the efficiency of xylose fermentation in lignocellulosic hydrolysate. The disclosed embodiment raises the efficiency of xylose conversion by adding a specific lignocellulosic material during fermentation. In particular, a 10% enhancement in the efficiency of xylose conversion for ethanol production was given, and the ethanol yield is achieved 90% after adding the specific lignocellulosic material.Type: ApplicationFiled: June 24, 2010Publication date: April 28, 2011Applicant: ATOMIC ENERGY COUNCIL-INSTITUTE OF NUCLEAR ENERGY RESEARCHInventors: Ting-Hsiang Lin, Chiung-Fang Huang, Gia-Luen Guo, Wen-Song Hwang
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Patent number: 7527964Abstract: A method for cleavage of the ether-linkage of polyethoxylates is described. A novel pure microbial culture, Pseudomonas nitroreducens TX1 (Depository No.: BCRC910228), is used under aerobic condition to cleave the ether-linkage adjacent to the carboxylated terminus or ethoxyl terminus. This method is useful to cleave sequentially ether-linkage of polyethoxylates in an aqueous buffer solution with an initial concentration of the polyethoxylates between 0.05% and 20%. This method is also effective for the cleavage of the ether-linkages in short-chain polyethoxylates with one to three ethoxyl units.Type: GrantFiled: June 22, 2004Date of Patent: May 5, 2009Assignee: National Central UniversityInventors: Shir-Ly Huang, Gia-Luen Guo
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Publication number: 20050075515Abstract: A method for cleavage of the ether-linkage of polyethoxylates is described. A novel pure microbial culture, Pseudomonas nitroreducens TX1 (Depository No.: BCRC910228), is used under aerobic condition to cleave the ether-linkage adjacent to the carboxylated terminus or ethoxyl terminus. This method is useful to cleave sequentially ether-linkage of polyethoxylates in an aqueous buffer solution with an initial concentration of the polyethoxylates between 0.05% and 20%. This method is also effective for the cleavage of the ether-linkages in short-chain polyethoxylates with one to three ethoxyl units.Type: ApplicationFiled: June 22, 2004Publication date: April 7, 2005Inventors: Shir-Ly Huang, Gia-Luen Guo