Abstract: The present invention relates to a method of ligating a first peptide via its C-terminus to the N-terminus of a second peptide, wherein the reaction is catalyzed by an asparagine/aspartate (Asx) peptide ligase OaAEPI Cys247Ala having the amino acid sequence of SEQ ID NO: 1. Further encompassed are a method of preparing a dimer, oligomer, or multimer of one or more peptides of interest and a method of modifying or tagging the surface of a target cell by one or more peptides of interest. Also encompassed in the invention are the ligated peptides and/or tagged target cells obtainable according to any of the methods, the peptide ligase OaAEPI Cys247Ala having the amino acid sequence of SEQ ID NO: 1, as well as kits comprising said peptide ligase.

Abstract: Factor VIII variants and methods of use thereof are disclosed. In particular embodiments, Factor VIII variants exhibit one or more improvements compared to wild-type Factor VIII proteins, including wild-type Factor VIII proteins with a B-domain deletion (FVIII-BDD). Examples may include enhanced activity or function, secretion at increased levels by cells or are packaged more efficiently into viral vectors.

Abstract: A piston has a piston crown and a top land extending circumferentially around the piston crown, as well as a combustion bowl and at least one valve pocket machined therein. The valve pocket has a side wall and a bottom, and wherein the side wall extends at an angle of between 110° and 120° from the bottom. A method for forming such valve pockets in the piston crown includes machining the valve pockets with a rotating cutter having an inverted frustoconical shape such that an angle between a bottom of the cutter and a side wall of the cutter amounts to between 110° and 120°.

Abstract: Techniques are described herein that are capable of reordering a multi-level layout using a hierarchical tree. A hierarchical tree is a tree that includes multiple hierarchical levels. Elements of a document may be arranged among the hierarchical levels to provide a logical representation of the document. The elements may be rearranged among the hierarchical levels to cause portions of content that are represented by the elements to be rearranged in a multi-level layout (e.g., a web page).

Abstract: The present invention relates to a method of forming a peptide of Formula (I) (P1-Xaa1-Xaa2-P2) by ligating a first peptide of Formula (II) (P1-Xaa1-X—R, wherein X is O or S) to a second peptide of Formula (II I) (Xaa1-Xaa2-P2) by enzymatically cleaving the bond between “Asx” and “X” in the first peptide of Formula (II) and ligating the fragment P1-Asx of the first peptide to the second peptide of Formula (III), wherein the enzymatic cleavage and ligation reaction is catalyzed by butelase 1 (SEQ ID NO: 1) and the peptide of Formula (I) is a depsipeptide, preferably a thiodepsipeptide. Further encompassed are peptides and dendrimeric peptide assemblies prepared using the presently disclosed method, as well as use of the dendrimeric peptide assemblies as a vaccine, medicament, or diagnostic agent, particularly as an antimicrobial agent.

Abstract: A method for controlling a hybrid vehicle including an internal combustion engine and electric motors each coupled to a wheel of a rear axle by a coupling device, and an electronic control unit connected to sensors, to a mechanism selecting a propulsion mode and also to the internal combustion engine and to the electric motors. The method includes: depending on running conditions of the vehicle and depending on the selected propulsion mode, controlling the coupling devices, controlling operation of the internal combustion engine and of the electric motors, and controlling torque of the internal combustion engine and of the electric motors.

Abstract: Technologies related to representing positions of graphical elements in a page through use of hierarchical position identifiers are described. A design canvas represents a page using zones, sections, and subsections, where a zone includes 1-m sections, and a subsection includes 1-p subsections. A position of a graphical element in a subsection is represented as a hierarchical position identifier, in the form of a tuple. The hierarchical position identifier includes an index value for a zone, an index value for a section, and an index value for a subsection. When the page is rendered, the graphical element is positioned in the page based upon its hierarchical position identifier.

Abstract: A piston has a piston crown and a top land extending circumferentially around the piston crown, as well as a combustion bowl and at least one valve pocket machined therein. The valve pocket has a side wall and a bottom, and wherein the side wall extends at an angle of between 110° and 120° from the bottom. A method for forming such valve pockets in the piston crown includes machining the valve pockets with a rotating cutter having an inverted frustoconical shape such that an angle between a bottom of the cutter and a side wall of the cutter amounts to between 110° and 120°.

Abstract: A piston for an internal combustion engine. The piston comprises a piston head and an articulated piston skirt. The piston head includes a crown and pin bosses depending from the crown. The piston skirt includes opposed thrust walls, opposed sidewalls, and a transverse bridge extending diametrically across the piston skirt, between the opposed sidewalls. A circumferential cooling gallery is encompassed within an outer circumferential ring belt section of the crown and is in fluid communication with a central cooling passage that extends between the crown and the piston skirt. The central cooling passage extends diametrically across the piston skirt, beneath at least a portion of the ring belt section and beneath at least a portion of an inner combustion bowl section of the crown.

Abstract: A user specified high level design selects a plurality of IP cores for placement in a customized system on a chip. A single integrated service automatically performs each of a design integration phase, specification phase, and verification phase for the user specified high level design to generate an integration file specifying stitching between a plurality of pins of each of the plurality of IP cores, a specification file specifying one or more characteristics of the customized system on a chip based on the user specified high level design, and a verification testbench for verification of the user specified high level design.

Abstract: A user specified high level design selects a plurality of IP cores for placement in a customized system on a chip. A single integrated service automatically performs each of a design integration phase, specification phase, and verification phase for the user specified high level design to generate an integration file specifying stitching between a plurality of pins of each of the plurality of IP cores, a specification file specifying one or more characteristics of the customized system on a chip based on the user specified high level design, and a verification testbench for verification of the user specified high level design.

Abstract: A user specified high level design selects a plurality of IP cores for placement in a customized system on a chip. A single integrated service automatically performs each of a design integration phase, specification phase, and verification phase for the user specified high level design to generate an integration file specifying stitching between a plurality of pins of each of the plurality of IP cores, a specification file specifying one or more characteristics of the customized system on a chip based on the user specified high level design, and a verification testbench for verification of the user specified high level design.

Abstract: A user specified high level design selects a plurality of IP cores for placement in a customized system on a chip. A single integrated service automatically performs each of a design integration phase, specification phase, and verification phase for the user specified high level design to generate an integration file specifying stitching between a plurality of pins of each of the plurality of IP cores, a specification file specifying one or more characteristics of the customized system on a chip based on the user specified high level design, and a verification testbench for verification of the user specified high level design.

Abstract: A method for monitoring/controlling starting of a heat engine of a hybrid vehicle including at least one electrical engine, including: launching a simulated sequence for starting the heat engine by using a start battery; measuring and recording progress over time of at least one parameter of the start battery, including a given current strength and voltage at terminals of the start battery; and detecting whether or not the progress over time of the at least one parameter reaches a predetermined value defining a state of the start battery, with or without a required capacity, for starting the heat engine during a predetermined time interval.

Abstract: A user specified high level design selects a plurality of IP cores for placement in a customized system on a chip. A single integrated service automatically performs each of a design integration phase, specification phase, and verification phase for the user specified high level design to generate an integration file specifying stitching between a plurality of pins of each of the plurality of IP cores, a specification file specifying one or more characteristics of the customized system on a chip based on the user specified high level design, and a verification testbench for verification of the user specified high level design.

Abstract: A method for controlling a hybrid vehicle including an internal combustion engine and electric motors each coupled to a wheel of a rear axle by a coupling device, and an electronic control unit connected to sensors, to a mechanism selecting a propulsion mode and also to the internal combustion engine and to the electric motors. The method includes: depending on running conditions of the vehicle and depending on the selected propulsion mode, controlling the coupling devices, controlling operation of the internal combustion engine and of the electric motors, and controlling torque of the internal combustion engine and of the electric motors.

Abstract: The present invention is directed to enzymes having Asx-specific ligase and cyclase activity and to nucleic acids encoding those as well as methods of the manufacture of said enzymes. Further encompassed are methods and uses of these enzymes.

Abstract: A piston for an internal combustion engine. The piston comprises a piston head and an articulated piston skirt. The piston head includes a crown and pin bosses depending from the crown. The piston skirt includes opposed thrust walls, opposed sidewalls, and a transverse bridge extending diametrically across the piston skirt, between the opposed sidewalls. A circumferential cooling gallery is encompassed within an outer circumferential ring belt section of the crown and is in fluid communication with a central cooling passage that extends between the crown and the piston skirt. The central cooling passage extends diametrically across the piston skirt, beneath at least a portion of the ring belt section and beneath at least a portion of an inner combustion bowl section of the crown.

Abstract: A user specified high level design selects a plurality of IP cores for placement in a customized system on a chip. A single integrated service automatically performs each of a design integration phase, specification phase, and verification phase for the user specified high level design to generate an integration file specifying stitching between a plurality of pins of each of the plurality of IP cores, a specification file specifying one or more characteristics of the customized system on a chip based on the user specified high level design, and a verification testbench for verification of the user specified high level design.

Abstract: A user specified high level design selects a plurality of IP cores for placement in a customized system on a chip. A single integrated service automatically performs each of a design integration phase, specification phase, and verification phase for the user specified high level design to generate an integration file specifying stitching between a plurality of pins of each of the plurality of IP cores, a specification file specifying one or more characteristics of the customized system on a chip based on the user specified high level design, and a verification testbench for verification of the user specified high level design.