Abstract: The present invention relates to a continuous fermentation process using Actinobacillus succinogenes. It was confirmed that in a cell recycled process, a production amount of succinic acid was about 60 g/L and productivity was about 3.873 g/L per hour. It was confirmed that a cell recycled fermentation process was increased in amount of microbial cells by about 2 times or more, increased in production amount of succinic acid by about 5 times, and increased in productivity of succinic acid by about 8 times or more as compared with the typical continuous culture. Such a succinic acid production process with high productivity and high yield rate can reduce production cost and can also produce succinic acid on an industrial level even at a pilot-scale culture unit without scaling up a culture unit. Therefore, if the process of the present invention is applied, it is expected to be more advantageous for industrial application.

Abstract: The present invention relates to a beta-glucan-based scaffold for biological tissue engineering using radiation fusion technology, and to a production method therefor. According to the production method of the present invention for beta-glucan-based scaffold, radiation fusion tissue engineering, a beta-glucan-aqueous solution is cast and is then irradiated in a crosslinking reaction in such a way as to form a gel or solid scaffold, thereby facilitating cell attachment and making it easy to create a biomimetic environment coinductive to the growth and differentiation of stem cells. Consequently, the beta-glucan-based scaffold according to the present invention can be usefully employed as a filler for tissue regeneration, cell culturing and plastic surgery, as a filler for voids in biological tissue, as a scaffold for reconstructive and corrective plastic surgery, and for cell transplantation and drug delivery.

Abstract: The present invention relates to a beta-glucan-based scaffold for biological tissue engineering using radiation fusion technology, and to a production method therefor. According to the production method of the present invention for beta-glucan-based scaffold, radiation fusion tissue engineering, a beta-glucan-aqueous solution is cast and is then irradiated in a crosslinking reaction in such a way as to form a gel or solid scaffold, thereby facilitating cell attachment and making it easy to create a biomimetic environment coinductive to the growth and differentiation of stem cells. Consequently, the beta-glucan-based scaffold according to the present invention can be usefully employed as a filler for tissue regeneration, cell culturing and plastic surgery, as a filler for voids in biological tissue, as a scaffold for reconstructive and corrective plastic surgery, and for cell transplantation and drug delivery.