Patents by Inventor Gijs van Rooijen

Gijs van Rooijen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 7666628
    Abstract: The present invention relates to the use of a class of genes called oil body protein genes that have unique features. The discovery of these features allowed the invention of methods for the production of recombinant proteins wherein a protein of interest can be easily separated from other host cell components. The invention is further exemplified by methods for exploitation of the unique characteristics of the oil body proteins and oil body genes for expression of polypeptides of interest in many organisms, particularly plant seeds. Said polypeptides may include but are not limited to: seed storage proteins, enzymes, bioactive peptides, antibodies and the like. The invention can also be modified to recover recombinant polypeptides fused to oil body proteins from non-plant host cells. Additionally the invention provides a method of using recombinant proteins associated with seed oil bodies released during seed germination for expression of polypeptides that afford protection to seedlings from pathogens.
    Type: Grant
    Filed: January 26, 2004
    Date of Patent: February 23, 2010
    Assignee: SemBioSys Genetics Inc.
    Inventors: Maurice M. Moloney, Gijs Van Rooijen
  • Patent number: 7642346
    Abstract: Novel methods for the expression of non-native genes in flax seeds and the seeds of other plant species are provided. The methods involve the use of seed-specific promoters obtained from flax. Additionally provided are novel flax seed-specific promoters, chimeric nucleic acid constructs comprising novel flax seed-specific promoters, transgenic plant cells, transgenic plants and transgenic plant seeds containing novel flax seed-specific promoters. The promoters and methods are useful, for example, for altering the seed oil and protein composition in flax seed or other plant seeds.
    Type: Grant
    Filed: March 19, 2004
    Date of Patent: January 5, 2010
    Assignees: Sembiosys Genetics Inc., Commonwealth Scientific and Industrial Research Organization (CSIRO)
    Inventors: Sarita Chaudhary, Gijs Van Rooijen, Maurice M. Moloney, Surinder Singh
  • Publication number: 20090282583
    Abstract: Commercial production of human insulin can be effected via transgenic expression in plant seeds. Thus, levels of insulin accumulation exceeding 0.1% of total cellular protein can be achieved recombinantly, through expression of the insulin with a single-chain antibody as a fusion partner. Production in seeds offers flexibility in storage and shipment of insulin as a raw material, and insulin retains its activity upon extraction from stored seed. Further, the amount of biomass subjected to extraction is limited, due to the relatively low water content of plant seeds.
    Type: Application
    Filed: May 12, 2009
    Publication date: November 12, 2009
    Applicant: SEMBIOSYS GENETICS INC.
    Inventors: MAURICE M. MOLONEY, JOSEPH BOOTHE, RICHARD KEON, CORY NYKIFORUK, GIJS VAN ROOIJEN
  • Patent number: 7585645
    Abstract: The present invention provides novel emulsion formulations which comprise oil bodies. The invention also provides a method for preparing the emulsions and the use of the emulsions in a variety of products including food products, personal care products and pharmaceutical products. In a preferred embodiment the emulsions comprise thioredoxin and/or thioredoxin reductase.
    Type: Grant
    Filed: July 5, 2001
    Date of Patent: September 8, 2009
    Assignee: Sembiosys Genetics Inc.
    Inventors: Harm M. Deckers, Gijs van Rooijen, Joseph Boothe, Janis Goll, Maurice M. Moloney, Bipin K. Dalmia
  • Publication number: 20090197337
    Abstract: Improved methods for the production of multimeric-protein-complexes, such as redox proteins and immunoglobins, in association with oil bodies are described. The redox protein is enzymatically active when prepared in association with the oil bodies. Also provided are related nucleic acids, proteins, cells, plants, and compositions.
    Type: Application
    Filed: December 19, 2001
    Publication date: August 6, 2009
    Inventors: Gijs Van Rooijen, Harm Deckers, Steven Briggs, Bipin Kumar Dalmia, Greg Del Val, Steve Zaplachinski, Maurice Moloney, Peter Bernard Heifetz
  • Patent number: 7547821
    Abstract: Commercial production of human insulin can be affected via transgenic expression in plant seeds. Thus, levels of insulin accumulation exceeding 0.1% of total cellular protein can be achieved recombinantly, through expression of the insulin with a single-chain antibody as a fusion partner. Production in seeds offers flexibility in storage and shipment of insulin as a raw material, and insulin retains its activity upon extraction from stored seed. Further, the amount of biomass subjected to extraction is limited, due to the relatively low water content of plant seeds.
    Type: Grant
    Filed: June 17, 2004
    Date of Patent: June 16, 2009
    Assignee: SemBioSys Genetics Inc.
    Inventors: Maurice M. Moloney, Joseph Boothe, Richard Keon, Cory Nykiforuk, Gijs Van Rooijen
  • Patent number: 7531325
    Abstract: An improved method for recovering recombinantly produced polypeptides is described. The method involves expressing the recombinant polypeptide as a fusion protein with a pro-peptide. The pro-peptide-polypeptide fusion protein can be cleaved and the recombinant polypeptide released under the appropriate conditions.
    Type: Grant
    Filed: December 19, 2002
    Date of Patent: May 12, 2009
    Assignee: SemBioSys Genetics Inc.
    Inventors: Gijs Van Rooijen, Joenel Alcantara, Maurice M. Moloney
  • Patent number: 7501265
    Abstract: An improved method for recovering recombinantly produced polypeptides is described. The method involves expressing the recombinant polypeptide as a fusion protein with a pro-peptide. The pro-peptide-polypeptide fusion protein can be cleaved and the recombinant polypeptide released under the appropriate conditions.
    Type: Grant
    Filed: April 23, 1998
    Date of Patent: March 10, 2009
    Assignee: SemBioSys Genetics Inc.
    Inventors: Maurice Moloney, Joenel Alcantara, Gijs Van Rooijen
  • Publication number: 20080184394
    Abstract: A method for the commercial production of chymosin which involves the recombinant expression of chymosin in plant seeds, that enables chymosin to accumulate to at least 0.5% (w/w) of total seed protein is described. An improved method for the isolation of chymosin from the seed is also described.
    Type: Application
    Filed: April 3, 2008
    Publication date: July 31, 2008
    Inventors: Gijs van Rooijen, Keon Richard Glenn, Yin Shen, Joseph Boothe
  • Patent number: 7390936
    Abstract: A method for the commercial production of chymosin which involves the recombinant expression of chymosin in plant seeds, that enables chymosin to accumulate to at least 0.5% (w/w) of total seed protein is described. An improved method for the isolation of chymosin from the seed is also described.
    Type: Grant
    Filed: August 23, 2000
    Date of Patent: June 24, 2008
    Assignee: SemBioSys Genetics Inc.
    Inventors: Gijs Van Rooijen, Keon Richard Glenn, Yin Shen, Joseph Boothe
  • Patent number: 7332587
    Abstract: A method for the separation of a target molecule from a mixture is described. The method employs oil bodies and their associated proteins as affinity matrices for the selective, non-covalent binding of desired target molecules. The oil body proteins may be genetically fused to a ligand having specificity for the desired target molecule. Native oil body proteins can also be used in conjunction with an oil body protein specific ligand such as an antibody or an oil body binding protein. The method allows the separation and recovery of the desired target molecules due to the difference in densities between oil bodies and aqueous solutions.
    Type: Grant
    Filed: October 1, 2002
    Date of Patent: February 19, 2008
    Assignee: SemBioSys Genetics Inc.
    Inventors: Maurice Moloney, Joseph Boothe, Gijs Van Rooijen
  • Patent number: 7098383
    Abstract: Improved methods for the production of multimeric-protein-complexes, such as redox proteins and immunoglobulins, in association with oil bodies are described. The redox protein is enzymatically active when prepared in association with the oil bodies. Also provided are related nucleic acids, proteins, cells, plants, and compositions.
    Type: Grant
    Filed: June 21, 2002
    Date of Patent: August 29, 2006
    Assignee: SemBioSys Genetics Inc.
    Inventors: Steven Szarka, Gijs Van Rooijen, Maurice Moloney
  • Patent number: 7091401
    Abstract: The present invention provides a method of preparing epidermal growth factors in plants and transgenic plant seeds containing epidermal growth factors. The method provides an economical way to produce epidermal growth factors.
    Type: Grant
    Filed: December 20, 2002
    Date of Patent: August 15, 2006
    Assignee: Sembiosys Genetics Inc.
    Inventors: Maurice M. Moloney, Gijs Van Rooijen
  • Patent number: 6924363
    Abstract: A method for the separation of a target molecule from a mixture is described. The method employs oil bodies and their associated proteins as affinity matrices for the selective, non-covalent binding of desired target molecules. The oil body proteins may be genetically fused to a ligand having specificity for the desired target molecule. Native oil body proteins can also be used in conjunction with an oil body protein specific ligand such as an antibody or an oil body binding protein. The method allows the separation and recovery of the desired target molecules due to the difference in densities between oil bodies and aqueous solutions.
    Type: Grant
    Filed: November 7, 2000
    Date of Patent: August 2, 2005
    Assignee: SemBioSys Genetics Inc.
    Inventors: Maurice Moloney, Joseph Boothe, Gijs van Rooijen
  • Publication number: 20050039235
    Abstract: Methods for the production of insulin in plants are described. In one embodiment, the present invention provides a method for the expression of insulin in plants comprising: (a) providing a chimeric nucleic acid construct comprising in the 5? to 3? direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant seed cells; and (ii) a nucleic acid sequence encoding an insulin polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and (c) growing the plant cell into a mature plant capable of setting seed wherein the seed expresses insulin.
    Type: Application
    Filed: June 17, 2004
    Publication date: February 17, 2005
    Inventors: Maurice Moloney, Joseph Boothe, Richard Keon, Cory Nykiforuk, Gijs Van Rooijen
  • Publication number: 20040255350
    Abstract: Novel methods for the expression of non-native genes in flax seeds and the seeds of other plant species are provided. The methods involve the use of seed-specific promoters obtained from flax. Additionally provided are novel flax seed-specific promoters, chimeric nucleic acid constructs comprising novel flax seed-specific promoters, transgenic plant cells, transgenic plants and transgenic plant seeds containing novel flax seed-specific promoters.
    Type: Application
    Filed: March 19, 2004
    Publication date: December 16, 2004
    Applicant: SemBioSys Genetic Inc.
    Inventors: Sarita Chaudhary, Gijs van Rooijen, Maurice M. Moloney, Surinder Singh
  • Publication number: 20040205844
    Abstract: The present invention relates to the use of a class of genes called oil body protein genes that have unique features. The discovery of these features allowed the invention of methods for the production of recombinant proteins wherein a protein of interest can be easily separated from other host cell components. The invention is further exemplified by methods for exploitation of the unique characteristics of the oil body proteins and oil body genes for expression of polypeptides of interest in many organisms, particularly plant seeds. Said polypeptides may include but are not limited to: seed storage proteins, enzymes, bioactive peptides, antibodies and the like. The invention can also be modified to recover recombinant polypeptides fused to oil body proteins from non-plant host cells. Additionally the invention provides a method of using recombinant proteins associated with seed oil bodies released during seed germination for expression of polypeptides that afford protection to seedlings from pathogens.
    Type: Application
    Filed: January 26, 2004
    Publication date: October 14, 2004
    Inventors: Maurice M. Moloney, Gijs van Rooijen
  • Patent number: 6777591
    Abstract: Novel methods for the expression of non-native genes in flax seeds and the seeds of other plant species are provided. The methods involve the use of seed-specific promoters obtained from flax. Additionally provided are novel flax seed-specific promoters, chimeric nucleic acid constructs comprising novel flax seed-specific promoters, transgenic plant cells, transgenic plants and transgenic plant seeds containing novel flax seed-specific promoters. The promoters and methods are useful, for example, for altering the seed oil and protein composition in flax seed or other plant seeds.
    Type: Grant
    Filed: August 25, 2000
    Date of Patent: August 17, 2004
    Assignees: SemBioSys Genetics Inc., Commonwealth Scientific and Industrial Research Organisation (CSIRO)
    Inventors: Sarita Chaudhary, Gijs van Rooijen, Maurico Moloney, Surindor Singh
  • Patent number: 6761914
    Abstract: The present invention provides novel adjuvants which comprise oil bodies. The invention also provides vaccine formulations comprising oil bodies and an antigen and methods for preparing the vaccines and the use of the vaccines to elicit an immune response.
    Type: Grant
    Filed: June 15, 2001
    Date of Patent: July 13, 2004
    Assignee: SemBioSys Genetics Inc.
    Inventors: Harm M. Deckers, Gijs van Rooijen, Joseph Boothe, Janis Goll, Maurice M. Moloney, Anthony B. Schryvers, Joenel Alcantara, Wendy A. Hutchins
  • Patent number: 6753167
    Abstract: The present invention relates to the use of a class of genes called oil body protein genes that have unique features. The discovery of these features allowed the invention of methods for the production of recombinant proteins wherein a protein of interest can be easily separated from other host cell components. The invention is further exemplified by methods for exploitation of the unique characteristics of the oil body proteins and oil body genes for expression of polypeptides of interest in many organisms, particularly plant seeds. Said polypeptides may include but are not limited to: seed storage proteins, enzymes, bioactive peptides, antibodies and the like. The invention can also be modified to recover recombinant polypeptides fused to oil body proteins from non-plant host cells. Additionally the invention provides a method of using recombinant proteins associated with seed oil bodies released during seed germination for expression of polypeptides that afford protection to seedlings from pathogens.
    Type: Grant
    Filed: June 29, 2001
    Date of Patent: June 22, 2004
    Assignee: SemBioSys Genetics Inc.
    Inventors: Maurice M. Moloney, Gijs van Rooijen