Patents by Inventor Giladi Locker

Giladi Locker has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Stabilized expression vectors containing .lambda.P.sub.L promoter and the gene for the cI.sub.434 repressor, plasmids containing the vectors, hosts containing the plasmids and related methods
    Patent number: 5059529
    Abstract: An improved vector upon introduction into a suitable host containing the thermolabile repressor C.sub.I renders the host capable of effecting expression of a desired gene. The vector is a double-stranded DNA molecule which includes in 5' to 3' order the following: the promoter and operator P.sub.L O.sub.L from lambda bacteriophage; the N utilization site; a first restriction enzyme site permitting replacement of the ribosomal binding site which follows thereafter; a ribosomal binding site; an ATG initiation codon or DNA which is converted into an ATG initiation codon upon insertion of the desired gene into the vector; a second restriction enzyme site for inserting the gene in phase with the ATG codon; a T.sub.1 T.sub.2 rRNA transcription termination sequence; an origin of replication; and a fragment designated cI.sup.434 on which is included the gene for the repressor protein and its associated promoter and operator.
    Type: Grant
    Filed: March 1, 1989
    Date of Patent: October 22, 1991
    Assignee: Bio-Technology General Corp.
    Inventors: Amos B. Oppenheim, Giladi Locker